Mutong Li^1,#, Xiaolian Yang^1,#, Zhishun Yu¹, Tingting Yuan¹, Wenjie Wang¹, Suqin Zhang^1,2,*, Guangdong Geng^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3175-3188, 2025, DOI:10.32604/phyton.2025.070731 - 29 October 2025

Abstract Wheat yield mainly depends on leaf photosynthesis and grain carbohydrate accumulation. The aux/indole-3-acetic acid 13-like (Aux/IAA13L) gene was successfully cloned from Tritipyrum ‘Y1805’ and transformed into common wheat. A bioinformatics analysis showed that the TtAux/IAA13L protein, encoding 232 amino acids, was hydrophilic and unstable. TtAux/IAA13L and Tel5E01G609500 were grouped together in a phylogenetic tree. The TtAux/IAA13L expression levels in the overexpression lines were higher than in the wild-type (WT) plants at five developmental stages: tillering, elongation, heading, flowering, and grain-filling. The expression levels in the overexpression lines first increased, peaked at the flowering stage, and then decreased.… More >

Farhat Abbas¹, Fahim Ullah Khan^1,2,*, Salem Al-Naemi³, Awni Al-Otoom¹, Ahmed T. Moustafa⁴, Khaled Shami¹

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2911-2925, 2025, DOI:10.32604/phyton.2025.064239 - 30 September 2025

Abstract This study aimed to explore the relationship between Soil-Plant Analysis Development (SPAD) values and key environmental factors in cucumber (Cucumis sativus L.) cultivation in a greenhouse. SPAD values, indicative of chlorophyll content, reflect plant health and productivity. The analysis revealed strong positive correlations between SPAD values and both indoor light intensity (ILI, r = 0.59, p < 0.001) and outdoor light intensity (OLI, r = 0.62, p < 0.001), suggesting that higher light intensities were associated with enhanced SPAD values. In contrast, significant negative correlations were found between SPAD values and soil temperature at 15–30 cm depth… More >

Jinge Li^1,2, Chuyao Wang^1,2, Fangbo Cao^1,2, Min Huang^1,2,*, Jiana Chen^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2155-2165, 2025, DOI:10.32604/phyton.2025.066940 - 31 July 2025

Abstract Grain filling is a critical determinant of yield and quality in rice. This study aims to clarify the association between grain photosynthesis and the filling rate of rice varieties with different grain weights, providing a theoretical foundation for optimizing grain-filling processes. Two rice varieties with similar growth duration but different grain weights were selected: a large-grain variety, Lingliangyou 268 (L268), and a small-grain variety, Ruiliangyou 1053 (R1053). Differences in grain filling, grain photosynthetic rate, and grain chlorophyll content were systematically examined during the filling stage. Results showed significant differences in grain-filling, grain photosynthetic rate, and… More >

Xianfeng Zhou^1,2,*, Ruiju Sun¹, Zhaojie Zhang¹, Yuanyuan Song¹, Lijiao Jin¹, Lin Yuan³

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 503-519, 2025, DOI:10.32604/phyton.2025.060827 - 06 March 2025

Abstract An accurate and robust estimation of leaf chlorophyll content (LCC) is very important to better know the process of material and energy exchange between plants and the environment. Compared with traditional remote sensing methods, abundant research has made progress in agronomic parameter retrieval using different CNN frameworks. Nevertheless, limited reports have paid attention to the problems, i.e., limited measured data, hyperspectral redundancy, and model convergence issues, when concerning CNN models for parameter estimation. Therefore, the present study tried to analyze the effects of synthetic data size expansion employing a Gaussian process regression (GPR) model for… More >

Yuanfen Gao, Tinglan Jiang, Yin Xiang, Xuewu He, Zhen Zhang, Shengjuan Wen, Junjie Zhang^*

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1465-1476, 2021, DOI:10.32604/phyton.2021.014811 - 27 May 2021

Abstract Brassinosteroids participate in many physiological processes in plants; however, their regulatory roles on the activities of the enzymes involved in dark phase of photosynthesis remains elusive. In this study, detached leaves and protoplasts of maize seedlings were treated with epi-brassinolide (EBR) and brassinazole followed by the determination of the contents of chlorophyll (a+b) and soluble sugars, and the activity of dark reaction enzymes and the expression of the relevant genes. The results showed that chlorophyll (a+b) content increased by 7.4% under 0.1 μM EBR treatment for 48 h; furthermore, chlorophyll (a+b) content increased by 34%… More >

Lijuan Kong^1,2, Haiye Yu^1,2, Zhaojia Piao^1,2, Meichen Chen^1,2, Jingmin Dang¹, Lei Zhang^1,2, Yuanyuan Sui^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1015-1030, 2021, DOI:10.32604/phyton.2021.014190 - 30 March 2021

Abstract Haze is mainly caused by the suspended particulate matters in the air, of which the particulate matters pollution harms leaf vegetables. In this paper, oilseed rapes at four different growing periods were investigated in a simulated particulate pollution environment. In combination of hyper-spectral technology and micro examination, the response of hyper-spectral characteristics of the leaf to particulate matters was investigated in-depth. The hyperspectral, chlorophyll content, net photosynthetic rate and stomatal conductance of leaf were obtained. The deposition and adsorption of particulate matters on the leaf were observed by Environmental Scanning Electron Microscope (ESEM). Normalized difference… More >

Abdulkhaliq Alshoaibi^*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 731-745, 2021, DOI:10.32604/phyton.2021.015476 - 30 March 2021

Abstract Seeds of three Saudi pearl millet cultivars (Pennisetum spicatum) from three regions (Madinah, Khulais and Jaizan) were inoculated with arbuscular mycorrhizal fungus Glomus mosseae obtained from the Agriculture Research Center of Giza, Egypt to enhance their salt tolerance. Five different NaCl concentrations (0, 30, 60, 90, and 120 mM) were used for treating cultivars with and without mycorrhiza. Growth rates, chlorophyll content, chlorophyll fluorescence (Fv/Fm), proline content and gas exchange were measured to determine the effect of salinity on these cultivars. The results indicated that compared to cultivars without mycorrhiza, all cultivars with mycorrhiza had enhanced growth and… More >

A. Sattar^1,*, A. Sher^1,*, M. Ijaz¹, M. Irfan², M. Butt¹, T. Abbas¹, S. Hussain², A. Abbas¹, M. S. Ullah³, M. A. Cheema⁴

Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 379-388, 2019, DOI:10.32604/phyton.2019.04784

Abstract Application of biochar to agricultural soils is mostly used to improve soil fertility. Experimental treatments were comprised of two factors: i) drought at two level, i.e., 80% and 40% water holding capacity (WHC) which was maintained on gravimetric basis ii) three levels of biochar i.e., control, 2 t ha^-1 and 4 t ha^-1 added to soil. Experimentation was done to examine potential of biochar application to enhance the growth attributes, water relations, photosynthetic pigments and antioxidants activities in maize (Zea mays L.) seedlings. Results of study revealed that biochar application increased the growth qualities (total seedlings biomass,… More >