Chuncheng Liu¹, Xiaohan Liu¹, Ziqi Li¹, Yanruoxue Wei¹, Bangdong Liu², Peng Zhu², Yukun Liu^1,2,*, Ran Zhao^1,2,*

Oncology Research, Vol.33, No.8, pp. 2085-2105, 2025, DOI:10.32604/or.2025.065739 - 18 July 2025

Abstract Background: VPS37A (VPS37A subunit of ESCRT-I), a component of the ESCRT-I (endosomal sorting complex required for transport I) complex, mediates vesicular trafficking through sorting endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Although accumulating evidence indicates that VPS37A deficiency occurs in numerous malignancies and exerts tumor-suppressive effects during cancer progression, its functional significance in colorectal cancer (CRC) pathogenesis remains poorly characterized. Therefore, this study aims to further investigate the functional and molecular mechanisms by which VPS37A downregulation contributes to malignant biological phenotypes in CRC, with a specific focus on how its dysregulation affects cell death pathways.… More > Graphic Abstract

YU ZHANG^1,2, QINGKUN WANG², YUE HAN², JUNJIE PIAO^2,*, XIUYING JIN^1,2,*

Oncology Research, Vol.33, No.8, pp. 1933-1945, 2025, DOI:10.32604/or.2025.063395 - 18 July 2025

Abstract Background: Colorectal adenocarcinoma (COAD) is one of the most common gastrointestinal malignancies. There is a pressing need to recognize reliable biomarkers that can improve diagnostic accuracy, predict prognosis, and serve as effective molecular targets. Glutathione peroxidase 4 (GPX4) is an important antioxidant protein. Evidence demonstrates that abnormal expression of GPX4 is related to cancer initiation and progression. However, the role of GPX4 in COAD remains unclear. Methods: We employed bioinformatics analysis and conducted subsequent validation of biological processes, including cell counting kit-8 assay (CCK-8), colony formation assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), 5-ethynyl-2′-deoxyuridine assay… More >

Fang Xu^1,2,#, Wan Li^1,#, Xiang-Jin Zheng^1,2, Yue Hao^1,2, Yi-Hui Yang^1,2, Hong Yang^1,2, Sen Zhang^1,2, Wan-Xin Cao^1,2, Xiao-Xue Li^1,2, Xu Zhang^1,2, Guan-Hua Du^1,2, Teng-Fei Ji^1,*, Jin-Hua Wang^1,2,*

Oncology Research, Vol.33, No.8, pp. 1969-1989, 2025, DOI:10.32604/or.2025.062386 - 18 July 2025

Abstract Objectives: Colorectal cancer (CRC) is one of the most common cancers all over the world. The progression of CRC is associated with inflammation and disruptions in intestinal flora. 3-O-Acetyl-11-keto-β-boswellic acid (AKBA) has been noted for its potent anti-inflammatory properties. However, the effect of AKBA on colon cancer caused by inflammation and its mechanism are not unclear. The study is to explore the effect of AKBA on CRC and its mechanism. Materials and Methods: Cell proliferation, (5-ethynyl-2^′-deoxyuridine, EdU)-DNA synthesis assay and colony formation were used to assess the effect of AKBA on the proliferation of CRC cells.… More >

Guo-Zhi Xu^1,2, Han-Yang Guan¹, Yan-Guan Guo¹, Yi-Ran Zhang¹, Jing-Hua Pan¹, Simin Luo³, Hui Ding¹, Yunlong Pan^1,*, Qi Yao^4,*

Oncology Research, Vol.33, No.8, pp. 1947-1967, 2025, DOI:10.32604/or.2025.060440 - 18 July 2025

Abstract Background: Tumor angiogenesis is related to solid tumor occurrence. Ubiquitin-specific peptidase 13 (USP13) is a deubiquitinating enzyme with a pivotal effect on tumor proliferation, metastasis, and tumorigenesis. Nonetheless, its effect on colorectal cancer (CRC) angiogenesis remains poorly understood. Methods: Human umbilical vein endothelial cells (HUVECs) and CRC cells were cultivated, followed by USP13 knockdown/overexpression using shRNA lentiviral vectors or plasmids. Conditioned media (CM) from treated CRC cells were collected to assess HUVEC migration, invasion, and tube formation. Phosphatase and tensin homolog (PTEN) overexpression and recombinant vascular endothelial growth factor A (VEGFA) rescue experiments were performed.… More >

RAQUEL GÓMEZ-MOLINA^1,*, RAQUEL MARTÍNEZ^2,3,4, MIGUEL SUÁREZ^2,3,4,*, ANA PEÑA-CABIA¹, MARíA CONCEPCIóN CALDERÓN¹, JORGE MATEO^3,4

Oncology Research, Vol.33, No.7, pp. 1531-1545, 2025, DOI:10.32604/or.2025.063951 - 26 June 2025

Abstract Lynch syndrome (LS), also known as hereditary non-polyposis colorectal cancer (HNPCC), is an inherited condition associated with a higher risk of colorectal cancer (CRC) and other cancers. It is caused by germline mutations in DNA mismatch repair (MMR) genes, including MLH1, MSH2, MSH6 and PMS2. These mutations lead to microsatellite instability (MSI) and defective DNA repair mechanisms, resulting in increased cancer risk. Early detection of LS is crucial for effective management and cancer prevention. Endoscopic surveillance, particularly regular colonoscopy, is recommended for individuals with LS to detect CRC at early stages. Additionally, universal screening of CRC for More > Graphic Abstract

Yuhang Li¹, Li Sun¹, Tao Wang¹, Bingjun Yu², Zhihong Gao³, Xiaochun Shu¹, Tengyue Yan¹, Weibing Zhuang^1,2,*, Zhong Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1211-1223, 2025, DOI:10.32604/phyton.2025.063647 - 30 April 2025

Abstract bHLH transcription factors, widely exist in various plants, and are vital for the growth and development of these plants. Among them, many have been implicated in anthocyanin biosynthesis across various plants. In the present study, a PdbHLH57 gene, belonging to the bHLH IIIf group, was characterized, which was isolated and cloned from the colored-leaf poplar ‘Zhongshancaiyun’ (ZSCY). The cDNA sequence of PdbHLH57 was 1887 base pairs, and the protein encoded by PdbHLH57 had 628 amino acids, the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa, respectively. Through bioinformatics analysis, PdbHLH57 has been classified… More >

XI ZHU^1,2,#, KAI HUANG^3,#, XIAOMING KAO², ZHAOHUI TANG³, WENJIE GUO³, TIANCONG WU^4,*, QIURONG LI^1,2,*

Oncology Research, Vol.33, No.5, pp. 1149-1159, 2025, DOI:10.32604/or.2024.054930 - 18 April 2025

Abstract Background: Colorectal cancer (CRC) holds the third position in global cancer prevalence mortality. Although chemotherapy is a conventional treatment, recent investigations have shed light on the therapeutic potential of the cGAS cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway in CRC management. Despite the primary role of the death domain-associated protein (Daxx) in cellular apoptosis, its influence on the regulation of cGAS-STING activation remains elusive. Methods: The Daxx degradation and speck formation were conducted using immunofluorescence and Western blotting. The Daxx knock-down and over-expression in CRC cells were performed to detect in vivo and in vitro… More >

Xiaojian Chen^1,2,#, Zhujiang Dai^1,#, Qiang Wang³, Wei Chen¹, Yun Liu^1,*, Zhongchuan Wang^1,*

BIOCELL, Vol.49, No.3, pp. 503-518, 2025, DOI:10.32604/biocell.2025.059286 - 31 March 2025

Abstract Background: Nudix Hydrolase 21 (NUDT21) is crucial for the regulation of alternative polyadenylation, with its reduced expression frequently resulting in a shortened mRNA 3^′ untranslated region (UTR), thereby enhancing the protein levels of downstream genes. Although NUDT21 is widely recognized for its tumor-suppressive function in various cancers, its involvement in colorectal cancer (CRC) remains poorly understood. Methods: The expression of NUDT21 in CRC and adjacent normal tissues was analyzed through qPCR, Western blot, and immunohistochemistry (IHC). Additionally, we investigated the correlation between NUDT21 expression and patient prognosis. With Cell Counting Kit-8 assay and Transwell assay, we… More >

Stellar Choi¹, HeeAe Ko², KyungRok Bae³, HyunSook Lee², HaeJong Joo⁴, Woong Choi^5,*

CMC-Computers, Materials & Continua, Vol.83, No.1, pp. 335-355, 2025, DOI:10.32604/cmc.2025.061243 - 26 March 2025

Abstract Previous research utilizing Cartoon Generative Adversarial Network (CartoonGAN) has encountered limitations in managing intricate outlines and accurately representing lighting effects, particularly in complex scenes requiring detailed shading and contrast. This paper presents a novel Enhanced Pixel Integration (EPI) technique designed to improve the visual quality of images generated by CartoonGAN. Rather than modifying the core model, the EPI approach employs post-processing adjustments that enhance images without significant computational overhead. In this method, images produced by CartoonGAN are converted from Red-Green-Blue (RGB) to Hue-Saturation-Value (HSV) format, allowing for precise adjustments in hue, saturation, and brightness, thereby… More >

JI-SU HAN¹, HYE-JIN BOO¹, JIN WON HYUN¹, HEESANG SONG², IN-YOUB CHANG³, SANG-PIL YOON^1,4,*

Oncology Research, Vol.33, No.4, pp. 873-884, 2025, DOI:10.32604/or.2024.052003 - 19 March 2025

Abstract Background: Chitosan oligosaccharide (COS) is the major degradation product of chitosan by enzymatic processes. COS, with complete water solubility, exerts significant biological effects, including anti-cancer activity. We investigated the anti-tumor effects of COS on colorectal cancer as effective therapeutic methods with low side effects are lacking. Methods: COS was obtained from low molecular weight chitosan by an enzymatic method and the anti-cancer effects were measured by cell viability assay, flow cytometry analysis, Western blotting, and xenograft. Results: COS suppressed the proliferation of SNU-C5 cells compared to other colorectal cancer cells, but higher concentrations were required in… More > Graphic Abstract