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  • Open Access

    ARTICLE

    Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves

    Olha Mykhailenko1,*, Ivan Bezruk1, Victoriia Volochai2, Volodymyr Mishchenko3, Liudas Ivanauskas4, Victoriya Georgiyants1

    Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 207-221, 2022, DOI:10.32604/phyton.2022.016458 - 16 August 2021

    Abstract The numerous studies indicate leaves of plants are a rich source of bioactive compounds that can be a valuable source of compounds used in the pharmaceutical and cosmetic industries. Aim of this study was to investigate the chemical composition and the antioxidant property of Crocus speciosus leaves. Primary phytochemical screening of C. speciosus leaves revealed the presence of some following compound categories such as phenolic compounds, aminoacids, saponins, proteins, tannins, triterpenoids, glycosides, polysaccharides. The total flavonoids and phenolic compounds content were determined spectrophotometrically and by HPLC-DAD and HPLC-MS. Antiradical activity was determined by ABTS radical-cation scavenging method,… More >

  • Open Access

    ARTICLE

    Chemical Constituents of Pedicularis longiflora var. tubiformis (Orobanchaceae), a Common Hemiparasitic Medicinal Herb from the Qinghai Lake Basin, China

    Feng Liu1,2,3, Zilan Ma1,2,3, Marcos A. Caraballo-Ortiz4, Hui Zhang5, Xu Su1,2,3, Yuping Liu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1083-1090, 2020, DOI:10.32604/phyton.2020.011239 - 09 November 2020

    Abstract Pedicularis longiflora var. tubiformis (Orobanchaceae) is an abundant parasitic herb mainly found in the Xiaopohu wetland of the Qinghai Lake Basin in Northwestern China. The species has an important local medicinal value, and in this study, we evaluated the chemical profile of its stems, leaves and seeds using mass spectrometry. Dried samples of stems, leaves and seeds were grinded, weighted, and used for a series of extractions with an ultrasonic device at room temperature. The chemical profiles for each tissue were determined using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid ChromatographyMass Spectrometry (LC-MS). Twenty-seven amino acids and organic… More >

  • Open Access

    ARTICLE

    Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

    S. J. Reyes-Zambrano1,†, M. L. Ramírez-Merchant1,†, C. Arias-Castro2, M. A. Rodríguez-Mendiola2, C. A. Lecona-Guzmán1, V. M. Ruíz-Valdiviezo1, D. González-Mendoza3, F. A. Gutiérrez-Miceli1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 277-284, 2019, DOI:10.32604/phyton.2019.06504

    Abstract A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets More >

  • Open Access

    ARTICLE

    Total Phenols, Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

    M. Y. Ovando-Domínguez1, M. C. Luján-Hidalgo1, D. González-Mendoza2, A. A. Vargas-Díaz3, N. Ruiz-Lau1,4, F. A. Gutiérrez-Miceli1, C. A. Lecona-Guzmán1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 139-147, 2019, DOI:10.32604/phyton.2019.06546

    Abstract Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content… More >

  • Open Access

    ARTICLE

    Colour, antioxidant capacity, phenolic and flavonoid content of honey from the Humid Chaco Region, Argentina

    Cabrera M1, M Perez2, L Gallez3,4, A Andrada3, G Balbarrey3

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 124-130, 2017, DOI:10.32604/phyton.2017.86.124

    Abstract Our objective was to correlate colour with total phenolic compounds, total flavonoids and antioxidant capacity of honey from the Humid Chaco region. With this purpose, 19 representative samples were selected from the 2009-2012 year period. Pollen analysis showed a predominance of native species of different landscapes such as forests and wetlands. Of the total number of samples, eleven were mixed or multifloral and eight samples were dominated by different native nectariferous woody species. The colour of honey, according to the Pfund scale, ranged from extra light amber to dark amber. Total phenolic content ranged from… More >

  • Open Access

    ARTICLE

    Synergism of flavonoids with bacteriostatic action against Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922

    MARÍA DE LOS A. ALVAREZ, NORA B. DEBATTISTA, NORA B. PAPPANO

    BIOCELL, Vol.30, No.1, pp. 39-42, 2006, DOI:10.32604/biocell.2006.30.039

    Abstract In our previous studies, bacteriostatic action of flavonoids against Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922 was demostrated. In the present work synergism of their combinations in order to improve the bacteriostatic action against the same microorganisms was determined. The experiences were made in nutritive broth, maintaining constant one drug concentration (20 µg/ml) and increasing the other one. A turbidimetric kinetic method was used and by means of a mechanism previously proposed, the minimal inhibitory concentrations (MIC´s) of each flavonoid combination were determined. The MIC´s for assayed combinations against S. aureus were: variable morin More >

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