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  • Open Access


    Cloning and Functional Validation of Mung Bean VrPR Gene

    Xiaokui Huang1, Yingbin Xue1, Aaqil Khan1, Hanqiao Hu1, Naijie Feng1,2,*, Dianfeng Zheng1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2369-2382, 2023, DOI:10.32604/phyton.2023.027457

    Abstract For the purpose of functional validation, the mung bean (Vigna radiata) VrPR gene was cloned and overexpressed in Arabidopsis thaliana. The findings revealed that the ORF of VrPR contained 1200 bp, in which 399 amino acids were encoded. Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts. The VrPR secondary structure comprised of random coil, α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure.… More >

  • Open Access


    Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation

    Yinglong Song1,#, Wenqian Shang1,#, Zheng Wang1,*, Songlin He1,2,*, Xinya Meng3, Liyun Shi1, Yuxiao Shen1, Dan He1, Xueyuan Lou1, Yuke Sun1

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2791-2812, 2022, DOI:10.32604/phyton.2022.023572

    Abstract Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony. In this study, on the basis of transcriptome sequencing, differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation. A total of 31.63 Gb raw data were generated and 120,188 unigenes (mean length of 911.98 bp) were annotated according to six databases (NR, NT, GO, KEGG, COG, and Swiss-Prot). Analyses of the ungerminated root primordium period, induced root primordium period, and root formation period detected 8,232, 6,907, and 10,687 differentially expressed genes related… More >

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