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Cloning and Functional Validation of Mung Bean VrPR Gene

Xiaokui Huang1, Yingbin Xue1, Aaqil Khan1, Hanqiao Hu1, Naijie Feng1,2,*, Dianfeng Zheng1,2,*

1 College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China
2 Innovation Research Center for Saline-Alkali Tolerant Rice, Shenzhen Research Institute of Guangdong Ocean University, Shenzhen, 518120, China

* Corresponding Authors: Naijie Feng. Email: email; Dianfeng Zheng. Email: email

Phyton-International Journal of Experimental Botany 2023, 92(8), 2369-2382. https://doi.org/10.32604/phyton.2023.027457

Abstract

For the purpose of functional validation, the mung bean (Vigna radiata) VrPR gene was cloned and overexpressed in Arabidopsis thaliana. The findings revealed that the ORF of VrPR contained 1200 bp, in which 399 amino acids were encoded. Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts. The VrPR secondary structure comprised of random coil, α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure. Moreover, analysis of the phylogenetic tree indicated that the soybean PR (Glyma.12G222200) and VrPR were closely related. Furthermore, chlorophyll content in leaves is markedly increased in Arabidopsis when VrPR is overexpressed. Our findings will serve as a reference for more functional studies on the PR genes in mung bean.

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APA Style
Huang, X., Xue, Y., Khan, A., Hu, H., Feng, N. et al. (2023). Cloning and functional validation of mung bean <i>vrpr </i>gene. Phyton-International Journal of Experimental Botany, 92(8), 2369-2382. https://doi.org/10.32604/phyton.2023.027457
Vancouver Style
Huang X, Xue Y, Khan A, Hu H, Feng N, Zheng D. Cloning and functional validation of mung bean <i>vrpr </i>gene. Phyton-Int J Exp Bot. 2023;92(8):2369-2382 https://doi.org/10.32604/phyton.2023.027457
IEEE Style
X. Huang, Y. Xue, A. Khan, H. Hu, N. Feng, and D. Zheng "Cloning and Functional Validation of Mung Bean <i>VrPR </i>Gene," Phyton-Int. J. Exp. Bot., vol. 92, no. 8, pp. 2369-2382. 2023. https://doi.org/10.32604/phyton.2023.027457



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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