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Cloning of the Soybean sHSP26 Gene and Analysis of Its Drought Resistance

Siyan Liu1,*, Jinfeng Liu1, Yuzhe Zhang1, Yushi Jiang1, Shaowang Hu1, Andi Shi1, Qiyao Cong1, Shuyan Guan2,*, Jing Qu2, Yao Dan1

1 College of Life Sciences, Jilin Agricultural University, Changchun, 130118, China
2 College of Agronomy, Jilin Agricultural University, Changchun, 130118, China

* Corresponding Authors: Siyan Liu. Email: email; Shuyan Guan. Email: email

Phyton-International Journal of Experimental Botany 2022, 91(7), 1465-1482. https://doi.org/10.32604/phyton.2022.018836

Abstract

Exploring the molecular mechanism of soybean response to drought stress, providing a basis for genetic improvement and breeding of heat-resistant varieties, relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18) and Jinong 18 mutant (JB18) soybeans, using reverse transcription, one gene in the sHSP family was cloned using PCR (RT-PCR) and it was named sHSP26. In this experiment, the soybean sHSP26 gene was successfully cloned by RT-PCR, the protein encoded by the sHSP26 gene was analyzed by bioinformatics, and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were constructed. The positive plants were derived from Agrobacterium-mediated transformation of soybean cotyledon nodes, and T2 plants were identified through conventional PCR, QT-PCR, and Southern blot hybridization. Finally, through the determination of drought-related physiological and biochemical indicators and the analysis of agronomic traits, further research on gene function was conducted. The results indicated that the overexpression vector plant GmsHSP26 gene expression increased. After stress, the SOD and POD activities, and the PRO content of the transgenic overexpression plants increased, while the MDA content decreased. The reverse was true for soybean plants with genetically modified editing vectors. A survey of agronomic traits indicated that the four-pod ratio and yield per plant of the transgenic overexpression plants were higher than those of the control and transgenic editing vector soybean plants. It indicates that the expression of the sHSP26 gene can enhance drought resistance and soybean yield. The soybean sHSP26 gene cloning and its functional verification have not yet been reported. This is the first report where PCR amplification of soybean sHSP26 genes and gene expression vector were applied. It lays the foundation for creating new drought-resistant transgenic soybean lines through genetic engineering technology and is essential for improving soybean yield and quality.

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APA Style
Liu, S., Liu, J., Zhang, Y., Jiang, Y., Hu, S. et al. (2022). Cloning of the soybean <i>shsp26</i> gene and analysis of its drought resistance. Phyton-International Journal of Experimental Botany, 91(7), 1465-1482. https://doi.org/10.32604/phyton.2022.018836
Vancouver Style
Liu S, Liu J, Zhang Y, Jiang Y, Hu S, Shi A, et al. Cloning of the soybean <i>shsp26</i> gene and analysis of its drought resistance. Phyton-Int J Exp Bot. 2022;91(7):1465-1482 https://doi.org/10.32604/phyton.2022.018836
IEEE Style
S. Liu et al., "Cloning of the Soybean <i>sHSP26</i> Gene and Analysis of Its Drought Resistance," Phyton-Int. J. Exp. Bot., vol. 91, no. 7, pp. 1465-1482. 2022. https://doi.org/10.32604/phyton.2022.018836



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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