ELHAM AMJAD¹, RAFFAELE PEZZANI^2,3,*, BABAK SOKOUTI^1,*

Oncology Research, Vol.32, No.3, pp. 439-461, 2024, DOI:10.32604/or.2023.044473

Abstract Noncoding RNAs instruct the Cas9 nuclease to site-specifically cleave DNA in the CRISPR/Cas9 system. Despite the high incidence of hepatocellular carcinoma (HCC), the patient’s outcome is poor. As a result of the emergence of therapeutic resistance in HCC patients, clinicians have faced difficulties in treating such tumor. In addition, CRISPR/Cas9 screens were used to identify genes that improve the clinical response of HCC patients. It is the objective of this article to summarize the current understanding of the use of the CRISPR/Cas9 system for the treatment of cancer, with a particular emphasis on HCC as part of the current state… More >

Hangxing Wang^1,#, Jingyun Fang^1,#, Yujiao Wang^2,#, Shuo Li², Zirui Wang², Wei He², Nan Wang¹, Shuang Luo¹, Huimei Zou^3,*, Fan Zhang^4,5,*

Oncologie, Vol.24, No.1, pp. 65-83, 2022, DOI:10.32604/oncologie.2022.021863

Abstract Lung cancer is the most common malignant tumor with the highest morbidity and mortality in the world, and non-small cell lung cancer (NSCLC) accounts for the vast majority of cases. At present, its main treatment methods are still traditional surgery, radiotherapy and chemotherapy, with disadvantages such as a high recurrence rate and limited effectiveness. Therefore, a new, better treatment method is urgently needed. Gene editing technology, as a new genetic engineering approach, has shown great potential in gene research, gene therapy and genetic improvement. It has also emerged as a promising treatment for lung cancer. This paper reviews the current… More >

BEEMNET MENGESHA KASSAHUN^1,#, BEUM-CHANG KANG^2,#, SU-JI BAE², YE JIN NAM¹, GRETEL FONSECA MUNDO¹, GA-HUI KANG¹, KYOUNGOOK KIM³, JEUNG-SUL HAN^1,4,*

BIOCELL, Vol.45, No.1, pp. 199-215, 2021, DOI:10.32604/biocell.2021.012353

Abstract Solanum lycopersicum ‘Heinz 1706’ is a pioneer model cultivar for tomato research, whose whole genome sequence valuable for genomics studies is available. Nevertheless, a genetic transformation procedure for this cultivar has not yet been reported. Meanwhile, various genome editing technologies such as transfection of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprotein complexes into cells are in the limelight. Utilizing the Cas9-expressing genotype possessing a reference genome can simplify the verification of an off-target effect, resolve the economic cost of Cas9 endonuclease preparation, and avoid the complex assembly process together with single-guide RNA (sgRNA) in the transfection approach. Thus,… More >