Yanhua Li^1,#, Hualei Huang^1,#, Youming Shi¹, Shuqin Huang¹, Tao Liu¹, Changming Xiao¹, Xiaoqing Tian², Ping Zhao¹, Xiaoyan Dai³, Taocui Huang^1,*, Yan Zhou^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 815-835, 2023, DOI:10.32604/phyton.2023.023928

Abstract Gibberellin 2-oxidases (GA2ox) are important enzymes that maintain the balance of bioactive GAs in plants. GA2ox genes have been identified and characterized in many plants, but these genes were not investigated in Brassica napus. Here, we identified 31 GA2ox genes in B. napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes. Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm, and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons. Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups, including… More >

XIN LI¹, JIANMIN PAN¹, FAISAL ISLAM², JUANJUAN LI¹, ZHUONI HOU¹, ZONGQI YANG¹, LING XU^1,*

BIOCELL, Vol.46, No.8, pp. 1947-1958, 2022, DOI:10.32604/biocell.2022.019806

Abstract NAC (NAM, ATAF, CUC) is a class of transcription factors involved in plant growth regulation, abiotic stress responses, morphogenesis and metabolism. Salvia miltiorrhiza is an important Chinese medicinal herb, but the characterization of NAC genes in this species is limited. In this study, based on the Salvia miltiorrhiza genomic databases, 82 NAC transcription factors were identified, which were divided into 14 groups. Meanwhile, phylogenetic analysis, gene structure, chromosomal localization and potential role of SmNACs in abiotic stress conditions were also studied. The results revealed that some SmNACs had different structures than others, which advised that these genes may have multiple/distinct… More >

JIANMIN PAN¹, XIN LI¹, JUANJUAN LI¹, HUI LIU², ZHUONI HOU¹, ZONGQI YANG¹, LING XU^1,*

BIOCELL, Vol.46, No.7, pp. 1751-1762, 2022, DOI:10.32604/biocell.2022.019455

Abstract Plant U-box (PUB) E3 ubiquitin ligases play important roles in hormone signaling pathways and in response to different abiotic stresses, but little is known about U-box genes in Danshen (root of Salvia miltiorrhiza Bunge). Here, we identified and characterized 70 SmPUB genes based on its genome sequence. Phylogenetic analysis of U-box genes from S. miltiorrhiza and Arabidopsis suggested that they can be clustered into seven subgroups (I–VII). Typical U-box domains were found in all identified SmPUB genes through the analysis of conserved motifs. Moreover, qRT-PCR was applied to analyze the relative expression levels of U-box genes in S. miltiorrhiza roots… More >

HUI-YING JIN^1,2, MAO-SONG PEI^1,2, DA-LONG GUO^1,2,*

BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718

Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, respectively. The… More >

Zhifeng Xiao, Yukun Jin, Qi Zhang, Abraham Lamboro, Baozhu Dong, Zhuoyu Yang, Piwu Wang^*

Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 349-361, 2022, DOI:10.32604/phyton.2022.017451

Abstract Soybean oleic acid content is one of the important indexes to evaluate the quality of soybean oil. In the synthesis pathway of soybean fatty acids, the FAD2 gene family is the key gene that regulates the production of linoleic acid from soybean oleic acid. In this study, CRISPR/Cas9 gene editing technology was used to regulate FAD2 gene expression. Firstly, the CRISPR/Cas9 single knockout vectors GmFAD2-1B and GmFAD2-2C and double knockout vectors GmFAD2-2A-3 were constructed. Then, the three vectors were transferred into the recipient soybean variety Jinong 38 by Agrobacterium-mediated cotyledon node transformation, and the mutant plants were obtained. Functional analysis… More >

Huifeng Li¹, Qiang Zhao², Hai Wang¹, Qinglong Dong^3,*, Yi Xu^4,*

Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 315-331, 2022, DOI:10.32604/phyton.2022.016951

Abstract BEL1-like homeodomain (BLH) family proteins are homeodomain transcription factors, which are found ubiquitously in plants and play important roles in regulating meristem and flower development. Although BLH proteins have been reported in some plant species, there is very little information available for plants in the Malus genus (e.g., apple tree:Malus domestica). In the present study, we identified 19 apple MdBLH genes. Phylogenetic analysis revealed that the MdBLH genes could be divided into five groups. Analysis of gene structure showed that MdBLH gene has four exons, and the third exon was 61 bp in length. Chromosomal location analysis suggested that the MdBLH… More >

Zunaira Sher¹, Muhammad Umair Majid¹, Sameera Hassan¹, Fatima Batool¹, Beenish Aftab^1,2, Bushra Rashid^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1613-1632, 2021, DOI:10.32604/phyton.2021.016154

Abstract Pakistan is facing the threat of Cotton Leaf Curl Virus (CLCuV) which is transmitted through whitefly to cotton crop. Molecular mechanism of leaf epicuticular wax protects the plants from different pathogens including insect attack and disease transmission. Objective of current study is the isolation and characterization of a wax related gene GaCyPI from Gossypium arboreum under CLCuV infection. A fragment of 475 bp was isolated from the total RNA and 3’ and 5’ RACE-PCR products were arranged by overlapping the extended sequences at both the ends. Deduced protein sequence of GaCyPI showed homology with Cyclophilin cis-trans isomerase gene of Gossypium… More >

Yanfei Bi¹, Bin Wei¹, Ying Meng², Zhongzhao Li², Zhenghui Tang¹, Feng Yin³, Chuntao Qian^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1161-1175, 2021, DOI:10.32604/phyton.2021.014396

Abstract Melon (Cucumis melo), belonging to the Cucurbitaceae family, is a globally important economic crop. GRAS (GAI, RGA, SCR) genes, which are a type of transcription factor, play a critical role in plant growth and development, including processes such as radial root patterning, light signalling, abiotic/biotic stress, axillary shoot meristem formation, and phytohormone (gibberellin) signal transduction. In this study, the GRAS family in melon was analysed comprehensively with respect to chromosomal location, motif prediction, gene structure, and expression pattern. A total of 37 GRAS genes were first identified in melon, after which a phylogenetic tree was built with the GRAS genes… More >

QICHAO WANG¹, WUJING ZENG¹, BASHARAT ALI², XUEMIN ZHANG³, LING XU^1,*, ZONGSUO LIANG^1,*

BIOCELL, Vol.45, No.4, pp. 1107-1119, 2021, DOI:10.32604/biocell.2021.015282

Abstract Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A. paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From… More >

LIYAN SU^1,2,#, HONG YU^3,#, JIUDONG ZHANG¹, XIAOHONG ZHAO¹, XUAN LIU¹, QIANG LI^1,4,*

BIOCELL, Vol.45, No.4, pp. 1153-1166, 2021, DOI:10.32604/biocell.2021.014499

Abstract Auxin signaling and its components (Auxin/Indole-3-Acetic Acid (Aux/IAA)) are critical for plant growth and development. Here, we performed a genome-wide annotation and identified twenty-one Aux/IAA genes in strawberry (Fragaria vesca). Most FveIAAs were located on chromosomes 1, 2, 4, 5, and 6, while no FveIAAs were found in chromosomes 3 and 7. Phylogenetic analysis divided these genes into nine subfamilies. Most FveIAAs contained the DNA-binding and Aux/IAA domains, as well as motifs I–IV. There were 2–6 exons in the FveIAA genes based on the gene structure analysis. Also, we found that four pairs of FveIAA genes underwent segment duplications. Moreover,… More >