Syed Ali Zafar^1,*, Muhammad Aslam², Haroon Zaman Khan³, Sehrish Sarwar¹, Rao Saad Rehman⁴, Mariam Hassan¹, Ramala Masood Ahmad², Rafaqat A. Gill⁵, Basharat Ali⁶, Ibrahim Al-Ashkar⁷, Abdullah Ibrahim⁷, Md Atikur Rahman⁸, Ayman El Sabagh^9,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1887-1906, 2023, DOI:10.32604/phyton.2023.027679

Abstract The availability of favorable genetic diversity is a thriving vitality for the success of a breeding program. It provides a firm basis of selecting superior breeding lines for the development of high yielding crop genotypes. In this context, present investigation aimed to generate information on genetic divergence and character association in a diversity panel of 123 local and exotic soybean genotypes. Analysis of variance revealed significant response of the evaluated genotypes based on studied attributes. It depicted the probability of selecting desirable soybean genotypes by focusing on character association studies and genetic diversity analysis. Correlation analysis revealed that seed yield… More >

Jameel M. Al-Khayri^1,*, Salha M. Alshamrani², Adel A. Rezk¹, Wael F. Shehata¹, Mustafa I. Almaghasla³, Tarek A. Shalaby^3,4, Ahmed M. Saad⁵, Fatmah A. Safhi⁶, Muhammad N. Sattar⁷, Arafat A. Abdel Latef⁸, Mahmoud Z. Sitohy⁵, Abdallah A. Hassanin^9,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1493-1512, 2023, DOI:10.32604/phyton.2023.027375

Abstract Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program. This study characterized fruit yield, quality attributes, and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions. Peto 86, Castle Rock, and Red Star cultivars showed the highest fruit yield (kg/plant), total phenolic compounds (TPC), and sap acidity. Principal component analysis categorized the evaluated fruit yield into three groups based on their quality attributes. A robust positive correlation appeared among traits inside each group. A positive correlation was likewise noticed between the first and the second groups. However, a negative correlation was… More >

Luli Li^1,2, Nigel G. Halford³, Huihui Wang⁴, Yingjie Zong¹, Zhenzhu Guo¹, Ruiju Lu¹, Chenghong Liu¹, Zhiwei Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1275-1287, 2023, DOI:10.32604/phyton.2023.026946

Abstract Barley (Hordeum vulgare L.) is an important economic crop for food, feed and industrial raw materials. In the present research, 112 barley landraces from the Shanghai region were genotyped using genotyping-by-sequencing (GBS), and the genetic diversity and population structure were analyzed. The results showed that 210,268 Single Nucleotide Polymorphisms (SNPs) were present in total, and the average poly-morphism information content (PIC) was 0.1642. Genetic diversity and population structure analyses suggested that these barley landraces were differentiated and could be divided into three sub-groups, with morphological traits of row-type and adherence of the hulls the main distinguishing factors between groups. Genotypes… More >

KHANDAKAR ABU MD MOSTAFIZAR RAHMAN^1,2, ABDUL SHUKOR JURAIMI^2,*, MD. REZWAN MOLLA³, MUHAMMAD ASYRAF MD HATTA⁴, ZULKEFLY BIN SULAIMAN⁵, SHAMIMA SULTANA⁶, AHMED GABER⁷, BENUKAR BISWAS⁸, AKBAR HOSSAIN^9,*

BIOCELL, Vol.47, No.1, pp. 227-237, 2023, DOI:10.32604/biocell.2023.025135

Abstract Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity. Therefore, molecular characterization of germplasms is important to obtain target cultivars with desirable traits. Sweet potato [Ipomoea batatas (L.) Lam] is widely considered the world’s most important crop, with great diversity in morphological and phenotypic traits. The genetic diversity of 20 sweet potato germplasms originating from Bangladesh, CIP, Philippines, Taiwan, and Malaysia were compared, which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization. This information was effective in differentiating… More >

XIAOJUN ZHOU^*, XIAOYU LU, XUBO WANG

BIOCELL, Vol.46, No.11, pp. 2489-2495, 2022, DOI:10.32604/biocell.2022.020311

Abstract Euptelea pleiosperma is a characteristic species of East Asian flora with both ornamental and scientific values. Based on the reduced-representation sequencing (RRS) technology of RAD-Seq, this study conducted high-throughput Illumina paired-end sequencing to find SSR marker information in the genome of E. pleiosperma, and to screen and verify polymorphism of SSR markers. We obtained 5.5G of high-quality data using RAD-Seq. The total number of contigs of the RAD tags was 299,376, with the maximum contig length of 2,062 bp and the average length of 445 bp. From these sequences, we identified 20,718 SSR loci, with a distribution density of one… More >

Sumei Li, Qing Wang, Peng Wang, Shuan Wang, Rutong Yang, Ya Li^*

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 617-632, 2022, DOI:10.32604/phyton.2022.017741

Abstract Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China. Understanding of genetic diversity plays an important role in C. oblongifolia survival and sustainable development. The wild C. oblongifolia population was artificially divided into four subpopulations according to the microhabitats, and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds. Then, the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms (SNPs) using specific-locus amplified fragment sequencing (SLAF-seq). Based on these SNPs, we aimed to… More >

DEBI RANI DATTA¹, MOHD RAFII YUSOP^1,2,*, AZIZAH MISRAN², MASHITAH JUSOH², YUSUFF OLADOSU¹, FATAI AROLU¹, AZADUL HAQUE¹, NADZIRAH M. SULAIMAN¹

BIOCELL, Vol.45, No.5, pp. 1393-1401, 2021, DOI:10.32604/biocell.2021.015321

Abstract Indo-Burmese region was the primary center of eggplant diversity from where the crop extended to several secondary origins of diversity. In this study, the genetic diversity among fifty-six eggplant accessions collected from three countries was assessed using sixteen polymorphic SSR markers to determine suitable parents for heterotic hybridization. The estimation of genetic diversity among the population of three countries (Bangladesh, Malaysia, and Thailand) varied from 0.57 to 0.74, with Shannon’s index value of 0.65. The mean value of expected heterozygosity and Nei’s index was 0.49, with an average PIC value of 0.83. A dendrogram was constructed based on UPGMA (unweighted… More >

HANNA AMOANIMAA-DEDE, JIACHENG ZHANG, CHUNTAO SU, HONGBO ZHU^*

BIOCELL, Vol.45, No.4, pp. 1095-1105, 2021, DOI:10.32604/biocell.2021.015053

Abstract Sweet potato is a multifunctional root crop with many essential nutrients and bioactive compounds. Due to its genetic complexity and lack of genomic resources, efficient genetic studies and cultivar development lag far behind other major crops. Simple sequence repeats (SSRs) offer an effective molecular marker technology for molecular-based breeding and for locating important loci in crop plants, but only a few have previously been developed in sweet potato. To further explore new SSR markers and accelerate their use in sweet potato genetic studies, genome-wide characterization and development of SSR markers were performed using the recently published genome of sweet potato… More >

MADE DIOUF¹, SARA DIALLO¹, FRANÇOIS ABAYE BADIANE^1,2, OUMAR DIACK¹, DIAGA DIOUF^1,*

BIOCELL, Vol.45, No.2, pp. 345-362, 2021, DOI: 10.32604/biocell.2021.013706

Abstract Cowpea is one of the most important legume grains in the sub-Saharan region of Africa used for human consumption and animal feed, but its production is hampered by biotic and abiotic constraints raising the need to broaden its genetic basis. For this purpose, seeds of two cowpea varieties Melakh and Yacine were irradiated with 300 and 340 Gy of gamma-ray, respectively. The developed mutant populations were agromorphologically characterized from M5 to M7, while the genetic diversity of the latter was evaluated using 13 ISSR markers. Based on the agromorphological characterization, variation of flower color, pod length, seed coat color, and… More >

ALI RAZA^1,7,#,*, ABU BAKR UMER FAROOQ^2,#, WAQAR AHMAD KHAN³, AHSAN IQBAL⁴, SADETTIN ÇELİK⁵, MAHWISH ALI⁶, RAO SOHAIL AHMAD KHAN^1,*

BIOCELL, Vol.44, No.4, pp. 769-776, 2020, DOI:10.32604/biocell.2020.010207

Abstract Randomly amplified polymorphic DNA (RAPD) is a tremendously convenient approach used to discriminate between Brassica species owing to its accuracy and speed. RAPD primers generate adequate genetic information that can be used in the primer-marker system. In this work, twenty RAPD-PCR based markers were executed to generate polymorphic data, like polymorphic information content (PIC), mean resolving power (MRP), resolving power (RP), effective multiplex ratio (EMR), and marker index (MI) for the first time and genetic distance among and between six Brassica species were calculated. Our results indicated that 20 primers produced a total of 231 scored band and generated 87%… More >