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  • Open Access

    ARTICLE

    Quantitative measures of leaf epidermal cells as a taxonomic and phylogenetic tool for the identification of Stanhopea species (Orchidaceae)

    Foroughbakhch R, RJ Ferry Sr, JL Hernández-Piñero, MA Alvarado-Vázquez, A Rocha-Estrada

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 113-127, 2008, DOI:10.32604/phyton.2008.77.113

    Abstract Orchids of the genus Stanhopea are currently identified only by their floral structure characteristics. A statistical analysis of a significant number of species of this genus disclosed that measurements of adaxial and abaxial epidermal cell surface areas can be correlated with specific recognized species by a leaf printing method. This allows an objectively either positive or nearly positive confirmation of the identity of a species in the absence of flowers and without damage to plants. When ordering the mean values obtained for these surface areas in each species in a decreasing order, a correlation was observed More >

  • Open Access

    ARTICLE

    Genetic and resistance stability to Black Sigatoka disease during micropopagation of Musa CIEN BTA-03 somaclonal variant

    Giménez1 C, E de García2, O Haddad3

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

    Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from More >

  • Open Access

    ARTICLE

    Variability in leaf morphology and anotomy in Lippia turbinata (Verbenaceae) in the Province of San Luis (Argentina)

    Andersen A, Lucchini F, J Moriconi, EA Fernández

    Phyton-International Journal of Experimental Botany, Vol.75, pp. 137-143, 2006, DOI:10.32604/phyton.2006.75.137

    Abstract Medicinal plants with mesomorphic and xeromorphic characteristics are a significant component of the indigenous flora of San Luis province. In popular medicine, Lippia turbinata “poleo” has emmenagogic, diuretic and stomach properties.
    The morphological and anatomical aspects of foliar leaves were studied according to their environmental conditions, in order to contribute to the identification of samples of commercial drugs. From the studied sections, differences in the following aspects were detected, namely the form and type of epidermic cells, the presence of idioblasts in adaxial epidermic position, thickness of cuticle, density of nonglandular trichomes, the structure of the More >

  • Open Access

    ARTICLE

    Influence of genotype and explant source on indirect organogenesis by in vitro culture of leaves of Melia azedarach L.

    S.K. VILA*, H.Y. REY, L.A. MROGINSKI

    BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

    Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l-1 benzylaminopurine (BAP) + 0.1mg.l-1 kinetin (KIN) + 3 mg.l-1 adenine sulphate (ADS).
    The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the More >

  • Open Access

    ARTICLE

    Water relations and leaf growth rate of three Agropyron genotypes under water stress

    MARÍA G. GARCÍA, CARLOS A. BUSSO, PABLO POLCI, NORBERTO L. GARCÍA GIROU, VIVIANA ECHENIQUE

    BIOCELL, Vol.26, No.3, pp. 309-317, 2002, DOI:10.32604/biocell.2002.26.309

    Abstract The effects of water stress on leaf water relations and growth are reported for three perennial tussock grass genotypes under glasshouse conditions. Studies were performed in genotypes El Palmar INTA and Selección Anguil of Agropyron scabrifolium (Döell) Parodi, and El Vizcachero of A. elongatum (Host) Beauv. Agropyron scabrifolium El Palmar INTA is native to a region with warm-temperate and humid climate without a dry season, and an average annual precipitation of 900 mm. Agropyron scabrifolium Selección Anguil comes from a region with a sub-humid, dry to semiarid climate and a mean annual precipitation of 600 mm. Agropyron elongatum is a… More >

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