Xiaona Li^1,*, Sijia Wang¹, Jie Song¹, Yixian Qin², Jizhong Yang³, Rui He^4,*, Weiyi Chen¹

Molecular & Cellular Biomechanics, Vol.18, No.1, pp. 11-19, 2021, DOI:10.32604/mcb.2021.014071

Abstract The cornea is a load-bearing tissue. Lower biomechanical properties in the local tissue of keratoconic cornea evoke mechanical stress increase. Inflammatory cytokines have been shown to be over-expressed in patients with keratoconus. However, how mechanical stimuli are involved in the production of inflammatory cytokines in keratoconus remains unclear. The objective of the study is to determine the role of mechanical stretch in the regulation of inflammatory cytokines and the underlying mechanisms in keratoconus. Human keratoconic fibroblasts (hKCFs) were subjected to 12% cyclic mechanical stretch at 0.1 Hz or in static conditions as controls. N-acetyl cysteine (NAC) and pyrrolidine dithiocarbamate and… More >

Guanbin Song^∗,†,‡, Qing Luo^*, Baiyao Xu^*, Yang Ju^†

Molecular & Cellular Biomechanics, Vol.7, No.3, pp. 165-174, 2010, DOI:10.3970/mcb.2010.007.165

Abstract It has been demonstrated that mechanical stimulation plays a vital role in regulating the proliferation and differentiation of stem cells. However, little is known about the effects of mechanical stress on tendon/ligament development from mesenchymal stem cells (MSCs). Here, using a custom-made cell-stretching device, we studied the effects of mechanical stretching on the cell morphology and mRNA expression of several key genes modulating tendon/ligament genesis. We demonstrate that bone-marrow-derived rat MSCs (rMSCs), when subjected to cyclic uniaxial stretching, express obvious detectable mRNAs for tenascin C and scleraxis, a unique maker of tendon/ligament formation, and significantly increased levels of type I… More >

Guanbin Song^∗,†,‡, Yang Ju^∗,†,§, Hitoshi Soyama^*, Toshiro Ohashi^¶, Masaaki Sato^¶

Molecular & Cellular Biomechanics, Vol.4, No.4, pp. 201-210, 2007, DOI:10.3970/mcb.2007.004.201

Abstract Mechanical stimulation is critical to both physiological and pathological states of living cells. Although a great deal of research has been done on biological and biochemical regulation of the behavior of bone marrow mesenchymal stem cells (MSCs), the influence of biomechanical factors on their behavior is still not fully documented. In this study, we investigated the modulation of mechanical stretch magnitude, frequency, and duration on the human marrow mesenchymal stem cells (hMSCs) proliferation by an in vitro model system using a mechanical stretch loading apparatus, and optimized the stretch regime for the proliferation of hMSCs. We applied 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrasodium… More >

V.B.Shim ¹, K. Mithraratne ¹

CMES-Computer Modeling in Engineering & Sciences, Vol.98, No.3, pp. 279-294, 2014, DOI:10.32604/cmes.2014.098.279

Abstract The activation of NF-kB is an important precursor in developing melanoma. However the role of mechanical stimulation in the NF-kB activation has not been studied. We used a multiscale computational modeling approach to investigate the role of mechanical stimulation and the skin tissue internal structures in the activation of NF-kB. Our model is made up of three levels – 1) the macro level where a FE model of the Zygomaticus major muscle was developed; 2) the meso level where a micro FE model of the skin block using a sample from human cadaver was developed; 3) the cell level where… More >