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  • Open Access

    ARTICLE

    B Class Floral Homeotic Genes are Involved in the Petal Identity and Flower Meristem Determinations in Chrysanthemum morifolium

    Jiayou Liu, Lian Ding, Xue Zhang, Song Li, Yunxiao Guan, Diwen Jia, Aiping Song, Jiafu Jiang, Fadi Chen*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 311-331, 2023, DOI:10.32604/phyton.2022.023896

    Abstract Chrysanthemum morifolium, an ornamental crop with diverse forms of inflorescence, is a good model for studying flower development in Asteraceae. However, the genetic background is complex and the mechanisms of regulating flower development are still unclear. Here, we identified two natural mutant lines of chrysanthemum and named them M1 and M2 according to the severity of the phenotype. Both lines showed defects in petal identity, and the petals of the M1 line had a mild phenotype: partially loss of petal identity and conversion of petals into green, leaf-like organs. The M2 line had severe phenotypes: in addition to severe petal… More >

  • Open Access

    ARTICLE

    Exploration and Validation of the Potential Downstream Genes Underlying ipa1-2D Locus for Rice Panicle Branching

    Lin Zhang1,2,*, Dong Xie1, Zhong Bian1, Yiting Zou1, Han Zhou1, Wenlu Cai1, Hadi Yeilaghi1, Xiaolei Fan1,2, Changquan Zhang1,2, Qiaoquan Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 773-787, 2021, DOI:10.32604/phyton.2021.015229

    Abstract In recent years, some super hybrid rice varieties were bred with strong culms and large panicles, which are mainly contributed by the ipa1-2D locus. A gain-of-function allele of OsSPL14 is the ipa1-2D and it can greatly increase the panicle primary branch number. However, the key downstream genes mediating this trait variation are not fully explored. In this study, we developed high-quality near-isogenic lines (NILs) with a difference of only 30 kb chromosomal segment covering the ipa1-2D locus. Using the NILs, we explored the impact of ipa1-2D on five sequential stages of early inflorescence development, and found that the locus can… More >

  • Open Access

    ARTICLE

    Chromium Differentially Affects Hydrogen Peroxide Distribution in Primary and Adventitious Roots of Arabidopsis thaliana L.

    Aarón Giovanni Munguía-Rodríguez1,2, José López-Bucio1, Gerardo Rangel Sánchez1,2, León Francisco Ruiz-Herrera1, Yazmín Carreón-Abud2, Miguel Martínez-Trujillo2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 35-43, 2020, DOI:10.32604/phyton.2020.07835

    Abstract The post-embryonic growth of the Arabidopsis thaliana root system can be modified by different types of stress, such as sublethal concentrations of metals, which may induce the production of reactive oxygen species (ROS). In this study, the effects of different concentrations of potassium chromate (KCrO4) on the distribution and relative quantity of hydrogen peroxide (H2O2) were determined in primary and adventitious roots in A. thaliana HyPer line seedlings. This line has a biosensor that specifically reports H2O2 levels within tissues as fluorescence. Primary root growth was inhibited at 100 μM Cr (VI); in contrast, adventitious root formation was induced over… More >

  • Open Access

    ARTICLE

    Cytogenetical and ultrastructural effects of copper on root meristem cells of Allium sativum L.

    DONGHUA LIU1*, WUSHENG JIANG2, QINGMIN MENG1, JIN ZOU1, JIEGANG GU1, MUAI ZENG1

    BIOCELL, Vol.33, No.1, pp. 25-32, 2009, DOI:10.32604/biocell.2009.33.025

    Abstract Different copper concentrations, as well as different exposure times, were applied to investigate both cytogenetical and ultrastructural alterations in garlic (Allium sativum L.) meristem cells. Results showed that the mitotic index decreased progressively when either copper concentration or exposure time increased. C-mitosis, anaphase bridges, chromosome stickiness and broken nuclei were observed in the copper treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. Also,… More >

  • Open Access

    ARTICLE

    An efficient protocol for culturing meristems of sorghum hybrids

    Sadia1,3 B, PC Josekutty2, SD Potlakayala2, P Patel2, S Goldman3, SV Rudrabhatla2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 177-181, 2010, DOI:10.32604/phyton.2010.79.177

    Abstract A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype… More >

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