BIN LI^1,2,3,#, YUCHENG SHENG^4,#, XIAOYING XU⁴, SHENGCUN WANG³, HONGYAN SONG³, JINGYUAN LI³, HAONAN JI¹, QINGHUA WANG³, XIAODI ZHOU^1,*, LONGJU QI^2,*

BIOCELL, Vol.48, No.4, pp. 601-611, 2024, DOI:10.32604/biocell.2024.047404

Abstract Objective: Chronic kidney disease (CKD) is a progressive disorder characterized by intricate structural and functional alterations in the kidneys, attributable to diverse causative factors. Notably, the therapeutic promise of miR-145-5p in addressing renal pathologies has been discerned. This investigation seeks to elucidate the functional role of miR-145-5p in injured kidneys by subjecting human glomerular mesangial cells (HGMCs) to stimulation with Angiotensin II (AngII). Materials and Methods: Cellular viability and the levels of inflammatory mediators were evaluated utilizing Cell Counting Kit-8 (CCK-8), quantitative real-time polymerase chain reaction (qRT-PCR), and western blot methodologies, both in the presence of AngII incubation and in… More >

Linpei Wang^1,#, Xiaoqiu Ma^2,#, Youqi Chen¹, Jiahui Zhang¹, Jiawei Zhang¹, Wei Wang^1,*, Shaojian Chen^3,*

Oncologie, Vol.24, No.4, pp. 897-912, 2022, DOI:10.32604/oncologie.2022.025693

Abstract Background: MicroRNA-145-5p (miR-145-5p) reportedly inhibits hepatocellular carcinoma (HCC) by targeting ARF6, SPATS2, CDCA3, KLF5, and NRAS, indicating that miR-145-5p plays an important role in the occurrence and development of HCC by regulating the expression of various genes. In this study, we aimed to explore novel downstream targets of miR-145-5p and elucidate the potential mechanism of miR-145-5p in HCC. Materials and Methods: A bioinformatics analysis was performed to determine the clinical significance of miR-145-5p and alpha/beta hydrolase domain-containing protein 17C (ABHD17C) in patients with HCC. The ability of Hep3B cells to proliferate, migrate, and invade was examined after overexpression of miR-145-5p… More >