SIMA GODOSEVICIUS KATZ

BIOCELL, Vol.29, No.3, pp. 261-270, 2005, DOI:10.32604/biocell.2005.29.261

Abstract The interaction of antimesometrial decidual cells and collagen fibrils was studied by light microscopy and ultrastructural cytochemistry in fed and acutely fasted mice on days 9-11 of pregnancy.
Fibrillar elements in the extracellular space consisted of collagen fibrils and filamentous aggregates (disintegrating collagen fibrils). Intracellular vacuoles exhibited typical collagen immersed in electron-translucent material (clear vacuoles) and faint cross-banded collagen immersed in electron-opaque material (dark vacuoles).
Fibrillar elements showed extracellular acid phosphatase activity which was stronger in the region of mature decidua than in predecidual cells region in all animals; it was conspicuous in mature decidua of fasted animals.… More >

JOEL ARNEODO*, SOLEDAD DE BREUIL*, SERGIO LENARDON**, LUIS CONCI**

BIOCELL, Vol.29, No.2, pp. 205-207, 2005, DOI:10.32604/biocell.2005.29.205

Abstract Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina. More >

MARÍA B. ESPINOSA² , NICOLÁS A. FRAUNHOFFER¹ , NOELIA P. LEOPARDO¹ , ALFREDO D. VITULLO² AND MIGUEL A. WILLIS¹

BIOCELL, Vol.35, No.2, pp. 37-42, 2011, DOI:10.32604/biocell.2011.35.037

Abstract Lagostomus maximus is a notable mammalian model for reproductive studies. Females have an extremely high ovulation rate, which is due to down-regulation of the follicular apoptosis pathway, which ensures a large pool of developing follicles. This large pool is supported by the convoluted anatomy of the mature ovary, whose germinal tissue is found in irregularly curved ridges throughout the cortex. Medullary tissue is restricted to a minimum. Lyso Tracker Red reconstruction under confocal laser scanning microscopy was used to recognize and measure all follicular stages from primordial to antral. Unlike most mammals in which early primordial follicles are just found… More >

Hajiboland R^1,2, C Poschenrieder³

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 86-100, 2015, DOI:10.32604/phyton.2015.84.086

Abstract Under acid soil conditions, solubility of aluminum (Al) increases leading to toxicity for plants. Al accumulator species such as tea, however, accumulate high levels of Al in tissues without toxicity symptoms. In this work, Al localization and compartmentation were studied in tea [Camellia sinensis (L.) O. Kuntze] grown hydroponically at 0 or 100 µM Al for eight weeks. Plant dry matter production was significantly higher in the presence of Al and accumulated up to 1.21 and 6.18 mg Al/g DW in the leaves and roots, respectively. About 40-50% of Al was partitioned into cell wall (CW)-bound fraction without any difference… More >

Orlando J. CASTEJÓN

BIOCELL, Vol.37, No.2, pp. 29-36, 2013, DOI:10.32604/biocell.2013.37.029

Abstract The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fi bers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells have functional ionotropic glutamate receptors… More >

Lidia M. Lopez*, Marta Dubin**, Patricia H. Carrizo**, Mario H. Burgos***, Amanda Pellegrino De Iraldi*, Andrés O. M. Stoppani**

BIOCELL, Vol.27, No.2, pp. 213-224, 2003, DOI:10.32604/biocell.2003.27.213

Abstract CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran- 5,6-dione; CG-NQ), a β-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen… More >

Dong Chen¹, Xiang Wang^1,*, Fuzhou Tang², Yajin Zhao¹

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 150-150, 2019, DOI:10.32604/mcb.2019.07071

Abstract Deformability is a fundamental property of the cells and tissues of living organisms, which is commonly detected to indicate the state of the cells. And the cell deformability usually depends on the methods that we used, which is easy to be confused. The present research is designed to explore the energy characteristic of red blood cell deformability, based on a quantitative analysis of extending-retracting curves acquired from atomic force microscopy. ATP-depleted red blood cells are prepared by treatment with free-glucose Ringer solution. Our results clearly show that the Youngs’ modulus of erythrocyte is closely depended on the concentration of intracellular… More >

Xueyi Yang¹, Yue Xu¹, Chun Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 101-101, 2019, DOI:10.32604/mcb.2019.07117

Abstract Cell-matrix interactions guide various cell behaviors, including proliferation, differentiation, migration, etc. Integrins, as a known transmembrane mechanosensor, undergo conformational changes in response to mechanical stimuli, and manipulate cell-matrix chemical-mechanical coupled signaling transduction [1]. The integrin-ligand bond kinetics has gain increasing attention among researchers. Independent studies showed that the integrin-ligand bond has been reported to be reinforced by the applied force f, while the loading rate df/dt had little effect on the bond lifetime [2].
We previously observed a dramatic increase in bond lifetime beyond a loading rate threshold for the integrin α2β1-DGEA bond, by introducing AFM (Atomic Force Microscopy)… More >

Dawei Song^1,*, Nicholas Hugenberg², Assad A Oberai¹

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 45-45, 2019, DOI:10.32604/mcb.2019.07138

Abstract Tractions exerted by cells on the extracellular matrix (ECM) are critical in many important physiological and pathological processes such as embryonic morphogenesis, cell migration, wound healing, and cancer metastasis. Traction Force Microscopy (TFM) is a robust tool to quantify cellular tractions during cell-matrix interactions. It works by measuring the motion of fiducial markers inside the ECM in response to cellular tractions and using this information to infer the traction field. Most applications of this technique have heretofore assumed that the ECM is homogeneous and isotropic [1], although the native ECM is typically composed of fibrous networks, and thus heterogeneous and… More >

Gloria B. Kim^1,†, Qiong Wei^2,†, Virginia Aragon-Sanabria¹, Sulin Zhang², Jian Yang¹, Cheng Dong^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 15-15, 2019, DOI:10.32604/mcb.2019.07137

Abstract Most cells survive and grow by attaching and spreading on a substrate. They generate internal tension that contracts the cell body and thus exert tractions on the underlying substrate through focal adhesions. Traction force also plays a critical role in many biological processes, such as inflammation, metastasis, and angiogenesis. Thus, measuring the cell traction force provides valuable information on understanding the underlying mechanism of these biological processes. Here, a traction force microscopy (TFM) method using super thin hydrogels composed of immobilized fluorescent beads was utilized to quantify the mechanical forces generated during the transmigration of Jurkat cells (a human T… More >