Zhichao Lou^1,2, Tiancheng Yuan¹, Qiuyi Wang¹, Xinwu Wu¹, Shouheng Hu¹, Xiaomeng Hao¹, Xianmiao Liu^3,*, Yanjun Li^1,*

Journal of Renewable Materials, Vol.9, No.5, pp. 959-977, 2021, DOI:10.32604/jrm.2021.014285

Abstract This work aimed to help the bamboo industry develop methodology for producing imperfection-free bamboo boards that can serve either decorative or structural benefit to consumers seeking to engage with the bioeconomy. Specifi- cally, softened and slotted bamboo tubes were handled by a roller device with nails to render crack-free flattened bamboo board. Softening temperature and time were optimized herein according to findings regarding chemical composition and board mechanical properties. The optimal softening parameters for saturated steam heat treatment is proved to be 160°C for 8 minutes. The flattened bamboo board possesses an increased bending strength of 101.5 MPa and a… More >

MARLENE BENCHIMOL^1,3,*, ABIGAIL MIRANDA-MAGALHÃES², ANTONIO PEREIRA-NEVES², WANDERLEY DE SOUZA³

BIOCELL, Vol.45, No.2, pp. 259-266, 2021, DOI:10.32604/biocell.2021.014599

Abstract Helium ion scanning microscopy (HIM) is a novel high-resolution scanning microscopy technique that uses helium ions instead of electrons to form images of the highest quality and resolution, providing a sub-nanometer resolution sputter uncoated biological cell. Here, we took advantage of HIM to explore the cell surface of Tritrichomonas foetus, a protist parasite of cattle that provokes hard infection and abortion in cows. We describe thin protrusions, like nanotubes described in other cells, with different sizes (27 nm to 81 nm in thickness) and various lengths (from 73 nm to 2 µm), as well bulbous structures either budding from the… More >

BIOCELL, Vol.27, Suppl.S, 2003

Abstract This article has no abstract. More >

RANDI ZUKAS¹, ALEX J. CHANG¹, MARIAN RICE², AMY L. SPRINGER^1*

BIOCELL, Vol.36, No.3, pp. 133-142, 2012, DOI:10.32604/biocell.2012.36.133

Abstract Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the α heavy chain, and IC138, an intermediate chain,… More >

MIROSLAV VAŘECHA^1*, DANIELA PÁCLOVÁ², JIŘINA PROCHÁZKOVÁ², PAVEL MATULA¹, DUŠAN CMARKO³, AND MICHAL KOZUBEK¹

BIOCELL, Vol.36, No.3, pp. 121-126, 2012, DOI:10.32604/biocell.2012.36.121

Abstract Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell… More >

ORLANDO J. CASTEJÓN^*

BIOCELL, Vol.36, No.1, pp. 1-29, 2012, DOI:10.32604/biocell.2012.36.001

Abstract The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts… More >

GISELE ORLANDI INTROÍNI¹, FERNANDA MARTINS MAESTER¹, FOSCA Pedini PEREIRA LEITE² AND SHIRLEI MARIA RECCO-PIMENTEL¹

BIOCELL, Vol.34, No.3, pp. 103-112, 2010, DOI:10.32604/biocell.2010.34.103

Abstract Ultrastructural analyses of bivalve spermatozoa are relevant in studies that aim to identify taxonomic traits for the purposes of discriminating species and conducting phylogenetic studies. In the present work, spermatozoa of mussel specimens of the genus Mytella, collected from two populations living in distinct habitats, were examined by electron microscopy. The objective was to identify sperm ultrastructural taxonomic traits that could be used to differentiate Mytella species. The specimens were from populations that live in intertidal zones on the southeast coast of Brazil, either buried in muddy-sand sediment or anchored to rocky substrates. The acrosomal vesicle was conical and long,… More >

SILVIA VILA, ANA GONZALEZ, HEBE REY AND LUIS MROGINSKI*

BIOCELL, Vol.34, No.1, pp. 7-14, 2010, DOI:10.32604/biocell.2010.34.007

Abstract Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical… More >

VIRGINIA VILLALOBOS¹, ERNESTO BONILLA^2,4,*, ALAN CASTELLANO³, ERNESTO NOVO¹, RALPH CASPERSEN⁴, DEBORA GIRALDOTH¹, SHIRLEY MEDINA-LEENDERTZ⁴

BIOCELL, Vol.33, No.3, pp. 187-197, 2009, DOI:10.32604/biocell.2009.33.187

Abstract The effect of manganese toxicity on the ultrastructure of the olfactory bulb was evaluated. Male albino mice were injected intraperitoneally with MnCl₂ (5 mg/Kg/day) five days per week during nine weeks. The control group received NaCl (0.9%). The olfactory bulbs of five mice from each group were processed for transmission electron microscopy after 2, 4, 6 and 9 weeks of manganese treatment. On week 2, some disorganization of the myelin sheaths was observed. After 4 weeks, degenerated neurons with dilated cisternae of rough endoplasmic reticulum and swollen mitochondria appeared. A certain degree of gliosis with a predominance of astrocytes with… More >

ORLANDO J. CASTEJÓN^1*, MICHAEL E. DAILEY²

BIOCELL, Vol.33, No.2, pp. 71-80, 2009, DOI:10.32604/biocell.2009.33.071

Abstract The localization of GluR1 subunits of ionotropic glutamate receptors in the glial cells and inhibitory neurons of cerebellar cortex and their association with the climbing and parallel fibers, and basket cell axons were studied. Samples of P14 and P21 rat cerebellar cortex were exposed to a specific antibody against GluR1 subunit(s) of AMPA receptors and were examined with confocal laser scanning microscopy. GluR1 strong immunoreactivity was confined to Purkinje cell and the molecular layer. Weak GluR1 immunoreactivity was observed surrounding some Golgi cells in the granule cell layer. Intense GluR1 immunoreactivity was localized around Purkinje, basket, and stellate cells. Purkinje… More >