Riaz Ahmad¹, Muhammad Akbar Anjum^1,*, Rashad Mukhtar Balal²

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 183-204, 2020, DOI:10.32604/phyton.2020.08537 - 22 April 2020

Abstract Molecular markers, genome sequencing and genome editing are considered as efficient tools to accomplish demands of plant breeders for crop improvement programs. Morphological and biochemical markers have not been extensively used as these are greatly influenced by environmental factors. Different molecular markers and sequencing techniques are routinely used in evaluation of genetic diversity and evolutionary relationship, accurate classification or taxonomy, characterization of germplasm, identification of hybrids and phylogenetic studies. Desired and undesired traits controlled by genes can be identified through different molecular markers technology all over the globe. These molecular markers are well established and… More >

Ning Xuan¹, Hua Zhang¹, Xu Liu¹, Qiang Guo¹, Jian Liu², Fangyin Yao^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 45-55, 2020, DOI:10.32604/phyton.2020.08548 - 01 March 2020

Abstract Rice blast disease is one of the most devastating diseases of rice (Oryza sativa L.) caused by the fungus Magnaporthe oryzae (M. oryzae), and neck blast is the most destructive phase of this illness. The underlying molecular mechanisms of rice blast resistance are not well known. Thus, we collected 150 rice varieties from different ecotypes in China and assessed the rice blast resistances under the natural conditions that favoured disease development in Jining, Shandong Province, China in 2017. Results showed that 92 (61.3%) and 58 (38.7%) rice varieties were resistant and susceptible to M. oryzae, respectively. Among the 150… More >

García-Zambrano EA¹, MC Ojeda-Zacarías¹, JG Gutiérrez-Soto¹, SR Sinagawa-García¹, A Gutiérrez-Díez¹, J Martínez-de la Cerda¹, JA Pedroza-Flores¹, C Gallegos-Vázquez², JI García-Zambrano³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that… More >

Ruíz-Salazar R¹, JS Muruaga-Martínez², MLP Vargas-Vázquez², G Alejandre-Iturbide⁴, G Castañón-Nájera³, S Hernández-Delgado¹, N Almaraz-Abarca⁴, N Mayek-Pérez^1,5

Phyton-International Journal of Experimental Botany, Vol.85, pp. 184-193, 2016, DOI:10.32604/phyton.2016.85.184

Abstract In order to identify genes of resistance to four diseases that affect Phaseolus sp. [i.e, Common Bacterial Blight (CBB), Bean Common Mosaic Virus (BCMV), Bean Golden Mosaic Virus (BGMV) and angular leaf spot (ALS)], we amplified nine SCAR (Sequence Characterized Amplified Regions) markers: four linked to resistance to common blight, two for BCMV, two for BGMV and one for ALS. The genetic material included 16 ayocote bean (P. coccineus) populations collected from different locations at Veracruz and Puebla, Mexico. Populations from Veracruz showed greater number of SCARs for BGMV and ALS compared with populations from Puebla. More >

Hernández-Ibáñez L, J Sahagún-Castellanos, JE Rodríguez-Pérez, MG Peña-Ortega, LM Rodríguez-Martínez

Phyton-International Journal of Experimental Botany, Vol.83, pp. 311-318, 2014, DOI:10.32604/phyton.2014.83.311

Abstract In many countries, tomato (Solanum lycopersicum L.) is the most important horticultural species, but good variety seeds are expensive. In hybrid breeding, the number of possible single crosses is too large with only a few lines. With 60 lines, for example, 1770 single-cross hybrids can be formed. This makes it expensive and even impossible to conduct an adequate experimental evaluation. These cases require the availability of methods for predicting hybrid performance. This study was designed to evaluate a method to predict fruit yield in tomato hybrids based on genomic fingerprints, the theory of mixed models, and… More >

Martínez ME¹, JM Lesher G², G Castañón N², E de la Cruz L¹, C Zapata H²

Phyton-International Journal of Experimental Botany, Vol.82, pp. 209-214, 2013, DOI:10.32604/phyton.2013.82.209

Abstract Nanche (Byrsonima crassifolia (L.) H.B.K.) grows in the southeastern region of the Mexican republic under both wild and semi-cultivated forms. Despite the importance of the fruit of this tree, only one paper at the molecular level has been published in Mexico, and possibly in America, to date. For this reason, the purpose of this research was to study the genetic variability of nanche trees that grow under both wild and semi-cultivated conditions in the state of Tabasco, Mexico. Molecular determinations were carried out in the Laboratorio de Genómica of the División Académica de Ciencias Biológicas (DACBiológicas),… More >

Escalante-González JG¹, EA García-Zambrano², A Gutiérrez-Diez², RE Vázquez-Alvarado², JA Torres-Castillo², SR Sinagawa-García², F Zavala-García², J Martínez-de la Cerda², C Gallegos-Vázquez³, JI García-Zambrano⁴

Phyton-International Journal of Experimental Botany, Vol.81, pp. 143-148, 2012, DOI:10.32604/phyton.2012.81.143

Abstract The objective of this research was to increase the genetic diversity of the Nopal Germplasm Bank of the FAUANL using RAPD molecular markers to estimate the genetic diversity of 15 accessions of cactus pear from molecular markers recorded in the Bank. DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit. Performance measurement of DNA was carried out through Picoflour. It was observed that the DNA concentrations ranged from 74.91 ng/µL to 12.32 ng/uL. Twelve primers were used to generate from 1 to 5 bands per primer with a molecular weight ranging from More >

Zappacosta D¹, M Meier¹, A Carrera¹, G Pacheco², S Cardone³, JP Selva¹, V Echenique¹

Phyton-International Journal of Experimental Botany, Vol.80, pp. 211-220, 2011, DOI:10.32604/phyton.2011.80.211

Abstract Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials… More >

Alejandre Rosas¹ JA, ME Galindo Tovar², HE Lee Espinosa², OG Alvarado Gómez³

Phyton-International Journal of Experimental Botany, Vol.79, pp. 87-94, 2010, DOI:10.32604/phyton.2010.79.087

Abstract Development of new sugarcane varieties allows to increase sucrose production and faces challenges as new pests and diseases, and soil fertility losses. The objective of this study was to contribute to expand molecular tools in selecting useful progenitors for hybridization programs. With this purpose, 14 RAPDs primers were pre-screened, and four of them which produced clearly resolved, reproducible and polymorphic fragment patterns were selected for genetic analysis of 22 sugarcane variety hybrids. These were used in the Estación de Hibridación at Tapachula, Chiapas, as progenitors in genetic improvement programs. Genetic distance analysis showed three main More >

García-Zambrano² EA, F Zavala-García², A Gutiérrez-Diez, MC Ojeda-Zacarías, I Cerda-Hurtado³

Phyton-International Journal of Experimental Botany, Vol.78, pp. 117-120, 2009, DOI:10.32604/phyton.2009.78.117

Abstract The objective of this study was to apply the AFLP-type molecular marker technique to estimate genetic diversity in cactus pear within the Germplasm Bank of FAUANL. Twelve accessions have been reported as possible duplicates through type RAPD- molecular markers in such Bank. DNA was extracted using the cellular ruptor technique, and then digested and bound. A preamplification and subsequent selective amplification were conducted. Amplified fragments were then separated and analyzed. It was concluded that none of the accessions was duplicated. This was because 8 groups were formed in the dendogram obtained after applying the UPGMA More >