G. Sriram¹, T. R. Ganesh Babu², R. Praveena^2,*, J. V. Anand³

Molecular & Cellular Biomechanics, Vol.19, No.1, pp. 29-40, 2022, DOI:10.32604/mcb.2022.016966

Abstract Leukemoid reaction like leukemia indicates noticeable increased count of WBCs (White Blood Cells) but the cause of it is due to severe inflammation or infections in other body regions. In automatic diagnosis in classifying leukemia and leukemoid reactions, ALL IDB2 (Acute Lymphoblastic Leukemia-Image Data Base) dataset has been used which comprises 110 training images of blast cells and healthy cells. This paper aimed at an automatic process to distinguish leukemia and leukemoid reactions from blood smear images using Machine Learning. Initially, automatic detection and counting of WBC is done to identify leukocytosis and then an automatic detection of WBC blasts… More >

JIRUI WEN^1,#, WENCHAO WU^2,#, MIN TANG¹, MINGYUE BAO¹, XUELING HE³, XINGHONG YAO¹, LIANG LI^1,*

BIOCELL, Vol.46, No.3, pp. 655-665, 2022, DOI:10.32604/biocell.2022.016915

Abstract To dissect which subset of bone marrow monocyte is the major precursor of osteoclast, 3-month-old rat bone marrow was obtained for single-cell RNA sequencing. A total of 6091 cells were acquired for detailed analysis, with a median number of 1206 genes detected per cell and 17,959 genes detected in total. A total of 19 cell clusters were recognized, with the main lineages identified as B cells, Granulocytes, Monocytes, T cells, Erythrocytes and Macrophages. Monocytes were further divided into classical monocytes and non-classical monocytes. Compared with non-classical monocytes, classical monocytes highly expressed osteoclast differentiation related genes Mitf, Spi1, Fos and Csf1r.… More >

XIANLIANG HOU^1,2,3,#, DONGE TANG^1,#, FENGPING ZHENG^1,#, MINGLIN OU³, YONG XU¹, HUIXUAN XU¹, XIAOPING HONG⁴, XINZHOU ZHANG¹, WEIER DAI⁵, DONGZHOU LIU^4,*, YONG DAI^1,*

BIOCELL, Vol.44, No.4, pp. 559-582, 2020, DOI:10.32604/biocell.2020.011022

Abstract Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by abnormal cellular and humoral immune responses and excessive autoantibody production. The precise pathologic mechanism of SLE remains elusive. The advent of single-cell RNA sequencing (scRNA-seq) enables unbiased analysis of the molecular differences of cell populations at the single-cell level. We used scRNA-seq to profile the transcriptomes of peripheral blood mononuclear cells from an SLE patient compared with a healthy control (HC). A total of 16,021 cells were analyzed and partitioned into 12 distinct clusters. The marker genes of each cluster and the four major immune cell types (B cells,… More >

CHANG SEOK LEE¹, YONG JAE SHIN¹, CHEOLHEE WON¹, YUN-SONG LEE², CHUNG-GYU PARK³, SANG-KYU YE^1*, MYUNG-HEE CHUNG¹

BIOCELL, Vol.33, No.2, pp. 107-114, 2009, DOI:10.32604/biocell.2009.33.107

Abstract Cyclooxygenase-2 (COX-2) is a key inflammatory response molecule, and associated with many immune functions of monocytes/macrophages. Particularly, interferon gamma (IFNγ)-induced COX-2 expression appears in inflammatory conditions such as viral infection and autoimmune diseases. Recently, statins have been reported to show variable effects on COX-2 expression, and on their cell and species type dependences. Based on the above description, we compared the effect of simvastatin on IFNγ-induced COX2 expression in human monocytes versus murine macrophages. In a result, we found that simvastatin suppresses IFNγ-induced COX-2 expression in human THP-1 monocytes, but rather, potentiates IFNγ-induced COX-2 expression in murine RAW264.7 macrophages. However,… More >