Hernández-León R¹, M Martínez-Trujillo², E Valencia-Cantero¹, G Santoyo¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 133-137, 2012, DOI:10.32604/phyton.2012.81.133

Abstract Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase,… More >

Rojas-Herrera R¹, M Zamudio-Maya¹, L Arena-Ortiz², RC Pless³, A O’Connor-Sánchez⁴

Phyton-International Journal of Experimental Botany, Vol.80, pp. 231-240, 2011, DOI:10.32604/phyton.2011.80.231

Abstract Mexico counts among the five countries with the highest biodiversity in the world. In the Yucatán Peninsula, there are aquatic ecosystems with a very special microbial diversity. These ecosystems are essential for the ecological equilibrium of the region, and are seriously threatened by human activities. Access and knowledge of the microbial resources of these environments have an enormous scientific interest, and could potentially result in biotechnological products which could lead to more efficient and environmentally friendly processes; it could also offer a full arsenal of microorganisms and/or novel molecules to the local and world industry to face the current needs.… More >

Hernández-León R, I Velázquez-Sepúlveda, MC Orozco-Mosqueda, G Santoyo

Phyton-International Journal of Experimental Botany, Vol.79, pp. 133-139, 2010, DOI:10.32604/phyton.2010.79.133

Abstract Soil is a complex system that includes a great number and diversity of microorganisms. Until recently, only a small percentage of the bioma was known and could be studied. Currently, it is possible to have a deeper knowledge of all that unknown genomic material with the development of new tools, like metagenomics. New molecules have been discovered with various biotechnological applications, and knowledge of the diverse microbiological interactions in several environments, some of them with extreme life conditions, is much higher. We analyze the most recent literature in the field of metagenomics in this study, especially that related with the… More >

Xie LB¹, X Wang¹, M Peng², Y Zhou¹, LX Chen¹, LX Liu³, YL Gao¹, YH Guo¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 236-245, 2017, DOI:10.32604/phyton.2017.86.236

Abstract Hot pepper (Capsicum annuum L.) is an important crop all over the world. To explore and identify differentially expressed proteins of hot pepper after space flight, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their control lines (W1 and W2), were analyzed using comparative proteome analysis. In this study, leaf morphological characteristics of five kinds of hot pepper variations were evaluated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results showed that obvious changes of cellular structure were seen in space induced mutants. Thirty nine out of the 255 differentially… More >

Annette Doyle^*, Steven R. Crosby^†, David R. Burton^*, Francis Lilley^*, Gary Johnston^*, Winder B. Perez^‡, Terri G. Kinzy^‡, Mark F. Murphy^*,†,§

Molecular & Cellular Biomechanics, Vol.10, No.4, pp. 275-288, 2013, DOI:10.3970/mcb.2013.010.275

Abstract Many diseases including cancer are associated with a disorganised cytoskeleton. The process of characterising how cytoskeletal disorganisation affects the mechanical properties of cells offers the potential to develop new drugs and treatment regimes that may exploit mechanical weakness in cells and tissues. This work investigated the role of actin associated proteins, namely tropomyosin 1 (tpm1p) and mitochondrial distribution and morphology protein 20 (mdm20p), on the mechanical and morphological properties of yeast cells. For the first time it was shown that deletion of both the TPM1 and MDM20 genes resulted in a decrease in Young’s modulus when compared to the wild-type… More >

Xinfang Wang¹, Lianqing Hong², Xi Wu³, Jia He³, Ting Wang^3,4,*, Hongbo Li⁵, Shaoling Liu⁶

CMC-Computers, Materials & Continua, Vol.61, No.1, pp. 141-154, 2019, DOI:10.32604/cmc.2019.06030

Abstract An ultrasonic nomogram was developed for preoperative prediction of Castleman disease (CD) pathological type (hyaline vascular (HV) or plasma cell (PC) variant) to improve the understanding and diagnostic accuracy of ultrasound for this disease. Fifty cases of CD confirmed by pathology were gathered from January 2012 to October 2018 from three hospitals. A grayscale ultrasound image of each patient was collected and processed. First, the region of interest of each gray ultrasound image was manually segmented using a process that was guided and calibrated by radiologists who have been engaged in imaging diagnosis for more than 5 years. In addition,… More >