MERVIN BURNETT¹, VITO RODOLICO², FAN SHEN¹, ROGER LENG¹, MINGYONG ZHANG³, DAVID D. EISENSTAT^4,5, CONSOLATO SERGI^1,3,6,*

BIOCELL, Vol.45, No.1, pp. 17-26, 2021, DOI:10.32604/biocell.2021.013973

Abstract MicroRNAs (miRNAs) are small single-stranded, non-coding RNA molecules involved in the pathogenesis and progression of cancer, including osteosarcoma. We aimed to clarify the pathways involving miRNAs using new bioinformatics tools. We applied WikiPathways and PathVisio, two open-source platforms, to analyze miRNAs in osteosarcoma using miRTar and ONCO.IO as integration tools. We found 1298 records of osteosarcoma papers associated with the word “miRNA”. In osteosarcoma patients with good response to chemotherapy, miR-92a, miR- 99b, miR-193a-5p, and miR-422a expression is increased, while miR-132 is decreased. All identified miRNAs seem to be centered on the TP53 network. This is the first application of… More >

HELIN FENG^1,2, XIAOCHONG ZHANG³, QIANQIAN ZHANG⁴, ZE LI⁵, LILI ZHAO^1,6,*

BIOCELL, Vol.44, No.3, pp. 443-450, 2020, DOI:10.32604/biocell.2020.010705

Abstract Osteosarcoma (OS) is a primary high-grade malignant bone neoplasm, and the prognosis of OS remains poor due to early metastasis. Leptin plays an essential role in tumorigenesis, but the role of leptin in the development of OS is still not fully understood. In this study, we used a human osteosarcoma MG-63 cell line as an experimental model. MG-63 cells were treated with leptin, and cell proliferation, apoptosis, adhesion, invasion, and gene expression, were evaluated. The results showed that leptin promoted proliferation, decreased adhesion, suppressed apoptosis, and promoted invasion, of MG-63 cells. Moreover, the expression of SIRT1 was upregulated in MG-63… More >

Hao Dang^*

Oncologie, Vol.22, No.1, pp. 35-42, 2020, DOI:10.32604/oncologie.2020.012485

Abstract This study aimed to explore the expression and diagnostic performance of miR-586 and miR-493 in patients with osteosarcoma. We enrolled 63 patients with osteosarcoma treated between May 2016 and May 2019 as the experimental group and 55 healthy volunteers who underwent physical examination during the same period as the control group. We detected miR-586 and miR-493 expression in serum, normal bone tissue, and tumor samples. We also evaluated the relationship between clinical indicators and the miR-586 and miR-493 expression levels. The correlation between the serum and osteosarcoma tissue expression of miR-586 and miR-493 was examined by Pearson analysis. Furthermore, the… More >

Hui Zhang, Zhan Wang, Lin Liu, Fuqiang Zhang, Yuxin Song, Yaowen Qian^*

Oncologie, Vol.22, No.1, pp. 23-33, 2020, DOI:10.32604/oncologie.2020.012493

Abstract The expression changes of miR-625-3p and miR-449a in osteosarcoma patients after surgery is the critical study in this paper. Analysis of their prognosis and risk factors can be helpful in understanding the prognostic value for clinical purposes. Fifty-eight patients with osteosarcoma diagnosed in our hospital were considered as the research group (RG), and 52 health subjects at the same time were collected as the control group (CG). Fluorescence quantitative PCR (RTPCR) was employed to test the expression levels of miR-625-3p and miR-449a in the serum of subjects in both groups before surgery and patients in the RG after surgery. The… More >