Kejun Wang^*1, Lin Yan^*1, Fen Lu^†

Oncology Research, Vol.27, No.2, pp. 157-163, 2019, DOI:10.3727/096504018X15190861873459

Abstract miR-363-3p has been shown to suppress tumor growth and metastasis in various human cancers. However, the function of miR-363-3p in osteosarcoma (OS) has not been determined. In our study, we found that the expression of miR-363-3p was significantly downregulated in OS tissues compared with adjacent normal tissues. miR-363-3p expression was associated with the poor overall survival rate of OS patients. Moreover, we found that overexpression of miR-363-3p markedly inhibited the proliferation, migration, and invasion of U2OS and MG63 cells. Moreover, we found that SOX4 was a direct target of miR-363-3p in OS cells. Overexpression of miR-363-3p significantly inhibited the expression… More >

Jian-Wei Wang, Xiao-Feng Wu, Xiao-Juan Gu, Xing-Hua Jiang

Oncology Research, Vol.27, No.9, pp. 979-986, 2019, DOI:10.3727/096504018X15336368805108

Abstract Cancer-associated fibroblasts (CAFs) play a predominant role in regulating tumor progression. Understanding how CAFs communicate with osteosarcoma is crucial for developing novel approaches for osteosarcoma therapy. Exosomes are able to transmit messages between cells. In this study, we demonstrated that CAFs transfer exosomes to osteosarcoma cells, which promotes osteosarcoma cell migration and invasion. Using a miRNA microarray analysis, we identified 13 miRNAs that are significantly increased in exosomes derived from cancer-associated fibroblasts (CAFs) and corresponding paracancer fibroblasts (PAFs). In vitro studies further validated that the levels of microRNA-1228 (miR-1228) were increased in CAFs, its secreted exosomes, and in recipient osteosarcoma… More >

Wenwei Jiang^*, Xinyu Cai^*, Tianyang Xu^*, Kaiyuan Liu^*, Dong Yang^*, Lin Fan^*, Guodong Li^*, Xiao Yu^†

Oncology Research, Vol.28, No.4, pp. 409-421, 2020, DOI:10.3727/096504020X15868639303417

Abstract Osteosarcoma (OS), the most common bone cancer, causes high morbidity in children and young adults. TRIM46 is a member of the family of tripartite motif (TRIM)-containing proteins that serve as important regulators of tumorigenesis. Here we investigate the possible role of TRIM46 in OS and the underlying molecular mechanism. We report an increase in the expression of TRIM46 in OS and its association with tumor size, Enneking’s stage, and patient prognosis. TRIM46 knockdown inhibits OS cell viability and cell cycle progression and induces apoptosis, while TRIM46 overexpression exerts inverse effects, which are inhibited by peroxisome proliferator-activated receptor alpha (PPAR )… More >

Hai-Feng Zeng^*, Hai-Yan Qiu^†, Fa-Bo Feng^*

Oncology Research, Vol.29, No.1, pp. 75-75, 2021, DOI:10.3727/096504022X16420719767471

Abstract This article has no abstract. More >

KANIZ FATEMA, ZACHARY LARSON, JARED BARROTT^*

BIOCELL, Vol.46, No.10, pp. 2177-2193, 2022, DOI:10.32604/biocell.2022.020141

Abstract Osteosarcoma is one of the most genomically complex cancers and as result, it has been difficult to assign genomic aberrations that contribute to disease progression and patient outcome consistently across samples. One potential source for correlating osteosarcoma and genomic biomarkers is within the non-coding regions of RNA that are differentially expressed. However, it is unsurprising that a cancer classification that is fraught with genomic instability is likely to have numerous studies correlating non-coding RNA expression and function have been published on the subject. This review undertakes the formidable task of evaluating the published literature of non-coding RNAs in osteosarcoma. This… More >

HONG FU¹, HAOYU ZHAO¹, YALI YANG¹, SIYU WANG¹, KE DUAN^2,*, TAILIN GUO^1,*

BIOCELL, Vol.46, No.4, pp. 1067-1078, 2022, DOI:10.32604/biocell.2022.017435

Abstract CDK5 belongs to the cyclin-dependent kinase family. CDK5 is multifunctional and plays an important role in neural differentiation. However, the role of CDK5 in osteoblastic differentiation remains unclear. The present study investigated functions and molecular mechanism of CDK5 in osteoblastic differentiation. It was found that, CDK5 inhibition promoted the expression of Runx2, ALP, OCN and OPN of MSCs and the mineralization of MC-3T3E1 cells and MSCs. CDK5 inhibition enhanced the development of F-actin, nuclear localization of β-catenin and YAP, as well as the expression of RMRP RNA. When F-actin was suppressed by Blebbistatin, the nuclear localization of YAP and β-catenin,… More >

FEI ZHANG^1,2,#, JIANZHONG LAI^3,#, RONGHAN HE^1,#, YI SHI⁴, KUN XU⁵, SHIHAI JIANG¹, TANGZHAO LIANG¹, WANYU ZHAO⁵, WEIDA REN⁵, LEI ZHU⁶, SONG JIN^2,*, KUN WANG^1,*

BIOCELL, Vol.46, No.3, pp. 699-709, 2022, DOI:10.32604/biocell.2022.015726

Abstract Circular RNAs (circRNAs) are a novel class of non-coding RNA that have recently shown to have huge capabilities in the regulation of gene expression at the posttranscriptional level. Growing evidence has indicated that circRNAs could serve as competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs) and suppress functions of targeted miRNAs. Osteosarcoma (OS) is the most common malignant primary bone cancer. Hsa_circ_0002137 is upregulated in OS. However, the role of hsa_circ_0002137 in OS remains unclear. Using miRNA pull-down assay, we showed that cir_0002137 sponged hsa-miR-1246, and BCL2 apoptosis regulator (BCL2) mRNA was a potential target of hsa-miR-1246 in human osteosarcoma… More >

Imran Ahmed^1,*, Humaira Sardar¹, Hanan Aljuaid², Fakhri Alam Khan¹, Muhammad Nawaz¹, Adnan Awais¹

CMC-Computers, Materials & Continua, Vol.69, No.3, pp. 3365-3381, 2021, DOI:10.32604/cmc.2021.018486

Abstract Osteosarcoma is one of the most widespread causes of bone cancer globally and has a high mortality rate. Early diagnosis may increase the chances of treatment and survival however the process is time-consuming (reliability and complexity involved to extract the hand-crafted features) and largely depends on pathologists’ experience. Convolutional Neural Network (CNN—an end-to-end model) is known to be an alternative to overcome the aforesaid problems. Therefore, this work proposes a compact CNN architecture that has been rigorously explored on a Small Osteosarcoma histology Image Dataaseet (a high-class imbalanced dataset). Though, during training, class-imbalanced data can negatively affect the performance of… More >

Lu Cao^1,*, Dianmei Yang², Bin Bai³

Oncologie, Vol.23, No.1, pp. 149-158, 2021, DOI:10.32604/oncologie.2021.014151

Abstract Objective: This study aimed to explore the miR-1247-mediated promotion of osteosarcoma (OS) cell proliferation by SOX9. Methods: We recruited 97 OS patients admitted to our hospital (the observation group, OG) and 82 healthy people undergoing physical examinations (the control group, CG) over the same period into this study. The expression of miR-1247 and SOX9 in human OS cells in vitro was tested to determine the effect of miR-1247 and SOX9 on OS and to analyze the relationship between miR-1247 and SOX9. Results: We detected lowly expressed miR-1247 and highly expressed SOX9 in the peripheral blood of OS patients (P <… More >

MERVIN BURNETT¹, VITO RODOLICO², FAN SHEN¹, ROGER LENG¹, MINGYONG ZHANG³, DAVID D. EISENSTAT^4,5, CONSOLATO SERGI^1,3,6,*

BIOCELL, Vol.45, No.1, pp. 17-26, 2021, DOI:10.32604/biocell.2021.013973

Abstract MicroRNAs (miRNAs) are small single-stranded, non-coding RNA molecules involved in the pathogenesis and progression of cancer, including osteosarcoma. We aimed to clarify the pathways involving miRNAs using new bioinformatics tools. We applied WikiPathways and PathVisio, two open-source platforms, to analyze miRNAs in osteosarcoma using miRTar and ONCO.IO as integration tools. We found 1298 records of osteosarcoma papers associated with the word “miRNA”. In osteosarcoma patients with good response to chemotherapy, miR-92a, miR- 99b, miR-193a-5p, and miR-422a expression is increased, while miR-132 is decreased. All identified miRNAs seem to be centered on the TP53 network. This is the first application of… More >