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MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line SAOS-2 via Targeting Caspase 8

Bin Xu, Hehuan Xia, Junming Cao, Zhihong Wang, Yipeng Yang, Yongsheng Lin

Department of Orthopedics, The Third Hospital of Hebei Medical University, Shijiazhuang, P.R. China

Oncology Research 2017, 25(7), 1161-1168. https://doi.org/10.3727/096504017X14841698396829

Abstract

Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line SAOS-2 was transfected with either miR-21 mimic or miR-21 inhibitor, and then cell viability, survival, and apoptosis were measured by MTT, colony formation assay, and flow cytometry. A target of miR-21 was predicted by the microRNA.org database and verified in vitro by using luciferase reporter, qPCR, and Western blot analyses. Finally, cells were cotransfected with siRNA against caspase 8 and miR-21 inhibitor, and the apoptotic cell rate was determined again. Results showed that the mRNA level of miR-21 was highly expressed in osteosarcoma tissues compared with adjacent tissues. Overexpression of miR-21 improved cell viability and survival but suppressed apoptosis. Caspase 8 was a direct target of miR-21, and it was negatively regulated by miR-21. Moreover, miR-21 suppression attenuated caspase 8 silencing and induced the decrease in apoptosis. In conclusion, overexpression of miR-21 suppressed SAOS-2 cell apoptosis via directly targeting caspase 8.

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APA Style
Xu, B., Xia, H., Cao, J., Wang, Z., Yang, Y. et al. (2017). Microrna-21 inhibits the apoptosis of osteosarcoma cell line SAOS-2 via targeting caspase 8. Oncology Research, 25(7), 1161-1168. https://doi.org/10.3727/096504017X14841698396829
Vancouver Style
Xu B, Xia H, Cao J, Wang Z, Yang Y, Lin Y. Microrna-21 inhibits the apoptosis of osteosarcoma cell line SAOS-2 via targeting caspase 8. Oncol Res. 2017;25(7):1161-1168 https://doi.org/10.3727/096504017X14841698396829
IEEE Style
B. Xu, H. Xia, J. Cao, Z. Wang, Y. Yang, and Y. Lin "MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line SAOS-2 via Targeting Caspase 8," Oncol. Res., vol. 25, no. 7, pp. 1161-1168. 2017. https://doi.org/10.3727/096504017X14841698396829



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