Lanjun Li¹, Degang Zhao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3043-3061, 2023, DOI:10.32604/phyton.2023.031175

Abstract Various strains of powdery mildew (PM), a notorious plant fungal disease, are prevalent and pose a significant threat to plant health. To control PM, transgenic technology can be used to cultivate more resistant plant varieties. In the present study, we utilized the rapid amplification of cDNA ends (RACE) technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance functions. Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase. The EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant, with the most abundant… More >

Jing Yang, Tianxiunan Pu, Ke Wan, Linqi Wang, Yuanshuai Shi, Xu Luo, Jie Tan, Dongmei Wang, Yang Liu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2555-2576, 2023, DOI:10.32604/phyton.2023.030301

Abstract Drought has severely affected the yield and quality of commercial crops. The BRI1 family plays an important role in plant response to drought stress, and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana. In this study, NtBRL3 was constructed as a vector and genetically transformed to obtain ‘N. Tobacco K326’ overexpression of NtBRL3. The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed. The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions, and 85 significantly differentially metabolites and 106… More >

Zijian Cao^#, Guoxia Xue^#, Lingfeng Hu, Haoxian Qu, Shuang Liang, Jisen Shi, Jinhui Chen^*, Zhaodong Hao^*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2383-2397, 2023, DOI:10.32604/phyton.2023.029845

Abstract The auxin polar transporter, PIN-FORMED 2 (PIN2) plays an important role in root development. However, it remains unclear whether PIN2 genes form two Liriodendron species, L. chinense (LcPIN2) and L. tulipifera (LtPIN2), are both involved in root development and whether and to what extent these two genes diverge in function. Here, we cloned and overexpressed LcPIN2 and LtPIN2 in Arabidopsis thaliana wild-type (WT) and Atpin2 mutant. Phylogenetic and sequence analysis showed a small degree of differentiation between these two Liriodendron PIN2 genes. Tissue-specific gene expression analysis indicated that both Liriodendron PIN2 genes were highly expressed in roots, implying a potential… More >

Chen Liu^1,2 , Xiaowei Tang^1,2, Xiaohui Qu^1,2 , Fadi Chen¹, Jiafu Jiang¹ and Sumei Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1391-1404, 2023, DOI:10.32604/phyton.2023.027231

Abstract Chrysanthemum morifolium Ramat is one of the four major cut flowers in the world. Pht1 family is focus on the uptake and transport of phosphate in plants. In our previous studies, CmPht1;2 overexpression line (Oe1) had higher phosphate contents both in roots and shoots, and its root development was significantly enhanced than wild type (WT) at low phosphorus conditions in chrysanthemum. Metabolomics analysis showed that several metabolites had a change in pyruvate metabolism and tricarboxylic acid (TCA) cycle pathway. To explore the gene difference expression and the change of metabolic pathway between CmPht1;2-Oe1 and WT, we conducted the transcriptome analysis.… More >

JINXIAN LIU¹, JINGFANG FENG², CHANG ZHANG², YONGJUAN REN², WEIHUA SU², GUANGHENG WU¹, XIANYU FU¹, NING HUANG³, YOUXIONG QUE², HUI LING^3,*, JUN LUO^2,*

BIOCELL, Vol.47, No.1, pp. 215-225, 2023, DOI:10.32604/biocell.2022.022477

Abstract Calmodulin (CaM) proteins play a key role in signal transduction under various stresses. In the present study, the effects of a sugarcane ScCaM gene (NCBI accession number: GQ246454) on drought and salt stress tolerance in transgenic Arabidopsis thaliana and Escherichia coli cells were evaluated. The results demonstrated a significant negative role of ScCaM in the drought and salt stress tolerance of transgenic lines of A. thaliana, as indicated by the phenotypes. In addition, the expression of AtP5CS and AtRD29A, two genes tightly related to stress resistance, was significantly lower in the overexpression lines than in the wild type. The growth… More >

Junsheng Sun^#, Haozhen Zhang^#, Mingxia Zhang, Sishen Li, Yanrong An^*

Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 79-90, 2023, DOI:10.32604/phyton.2022.022225

Abstract EARLY FLOWERING 3 (ELF3), a light zeitnehmer (time-taker) gene, regulates circadian rhythm and photoperiodic flowering in Arabidopsis, rice, and barley. The three orthologs of ELF3 (TaELF3-1AL, TaELF3-1BL, and TaELF3-1DL) have been identified in wheat too, and one gene, TaELF3-1DL, has been associated with heading date. However, the basic characteristics of these three genes and the roles of the other two genes, TaELF3-1BL and, TaELF3-1AL, remain unknown. Therefore, the present study obtained the coding sequences of the three orthologs (TaELF3-1AL, TaELF3-1BL, and TaELF3-1DL) of ELF3 from bread wheat and characterized them and investigated the role of TaELF3-1BL in Arabidopsis. Protein sequence… More >

YI DONG¹, LIANBO ZHANG², XIN GUAN³, TAO LIU¹, LIMIN ZHOU^1,*

Oncology Research, Vol.29, No.4, pp. 291-303, 2021, DOI:10.32604/or.2022.025172

Abstract Increasing numbers of long noncoding RNAs (lncRNAs) are implicated in breast cancer oncogenicity. However, the contribution of LINC02568 toward breast cancer progression remains unclear and requires further investigation. Herein, we evaluated LINC02568 expression in breast cancer and clarified its effect on disease malignancy. We also investigated the mechanisms underlying the pro-oncogenic role of LINC02568. Consequently, LINC02568 was upregulated in breast cancer samples, with a notable association with worse overall survival. Functionally, depleted LINC02568 suppressed cell proliferation, colony formation, and metastasis, whereas LINC02568 overexpression exerted the opposite effects. Our mechanistic investigations suggested that LINC02568 was physically bound to and sequestered microRNA-874-3p… More >