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  • Open Access

    ARTICLE

    Evaluation of Beta-Lactam Antibiotics on the Regeneration of Peanut Plants and Their Inhibitory Effect on Agrobacterium Growth

    Abraham Lamboro1,3,*, Songnan Yang1, Xueying Li1, Dan Yao2, Jun Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2489-2501, 2023, DOI:10.32604/phyton.2023.029492

    Abstract The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was tested using two peanut cultivars. The culture media contained 4 mg/L 6-benzylaminopurine (BAP) as the main growth regulator. Various concentrations (100–600 mg/L) of cefotaxime, carbenicillin, and timentin were applied in the culture media. In all the tested media, there were no significant differences in the shoot induction as compared to the control. However, little phytotoxic effect was observed at higher concentrations of these antibiotics in the shoot elongation media. Under shoot elongation medium, shoots turned brownish and partly died at higher concentrations where shooting rates… More >

  • Open Access

    ARTICLE

    Quantitative Trait Loci (QTL) Mapping and Marker Analysis of Fatty Acids in Peanut

    Xiao Han, Songnan Yang, Xueying Li, Qiulin Wu, Yongyi Xing, Jun Zhang*, Fenglou Ling*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2577-2589, 2023, DOI:10.32604/phyton.2023.029440

    Abstract Peanut, with high oil content, has been a major oil and food crop globally. The compositions of the fatty acids are the common factors in determining the oil quality. In the present study, an F2 segregated population with 140 individuals derived from the cross of Weihua8 (a cultivar) and 12L49 (a line with high oleic acid concentration) was used to construct a genetic map and conduct QTL mapping analysis. A total of 103 polymorphic SSR primers were utilized for genotyping the RILs and finally generating the SSR loci. Within the 103 SSR loci, a genetic linkage map, covering a total… More >

  • Open Access

    ARTICLE

    Preparation of Peanut Shell Cellulose Double-Network Hydrogel and Its Adsorption Capacity for Methylene Blue

    Yalin Li1,*, Lei Liu1, Wenbao Huang1, Junpu Xie2, Zhaoxia Song1, Shuna Guo1, Enci Wang1

    Journal of Renewable Materials, Vol.11, No.7, pp. 3001-3023, 2023, DOI:10.32604/jrm.2023.026604

    Abstract To achieve optimal recovery and value-added utilisation of cellulose in peanut shells, the cellulose in peanut shells was first extracted using the sodium hydroxide-sodium chlorite method. Then, cellulose hydrogel was prepared by graft copolymerisation using N, N’-methylenebisacrylamide as the cross-linking agent, sodium persulfate as the initiator, and acrylic acid as the monomer. Orthogonal optimisation experiments were designed to obtain optimal process parameters for hydrogel preparation with the cellulose dosage of 0.40 g, initiator dosage of 0.20 g, polymerisation temperature of 70°C, cross-linking agent of 0.25 g, and monomer dosage of 3.0 mL. The effect of initiator dosage on hydrogel synthesis was… More > Graphic Abstract

    Preparation of Peanut Shell Cellulose Double-Network Hydrogel and Its Adsorption Capacity for Methylene Blue

  • Open Access

    ARTICLE

    Effects of Tsukamurella tyrosinosolvens P9 on growth, physiology and antioxdant enzyme of peanut under drought stress and after re-watering

    CHANGMEI LONG, TINGTING YANG, YUJIE HAN, LIZHEN HAN*

    BIOCELL, Vol.47, No.6, pp. 1417-1430, 2023, DOI:10.32604/biocell.2023.027485

    Abstract Background: The plant-growth-promoting rhizobacterium Tsukamurella tyrosinosolvens is a rare strain of actinomycete, in order to recognize and expand the ecological functions of rare actinomycetes. Methods: In this experiment, we studied the effect of Tsukamurella tyrosinosolvens P9 on the drought resistance of peanut by inoculating peanut seedlings in pots and measuring the growth and physiological indicators of peanut under drought stress and re-watering conditions. Results: The results showed that during drought stress, the relative water content of the soil and leaves, chlorophyll content, and stomatal length, width, and aperture were significantly decreased while the levels of malondialdehyde (MDA), H2O2 and stomatal… More >

  • Open Access

    ARTICLE

    Construction of EMS-Induced Peanut Mutant Libraries and Identification of Pod-Related Traits Mutant Lines

    Hao Chen1, Faqian Xiong2,*, Rilong Xu1, Xiangyu Chen1, Haifeng Zhong1, Yumei Zhang1, Xinlong Lan1, Hong Zhang1, Yuhua Chen1, Runfang Hu1, Guoqiang Lin1,*, Zhaoxiu Tang1

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 537-557, 2023, DOI:10.32604/phyton.2022.023912

    Abstract Peanut (Arachis hypogaea L.) is an oil and economic crop of vital importance, and peanut pod is the key organ influencing the yield and processing quality. Hence, the Pod-related traits (PRTs) are considered as important agronomic traits in peanut breeding. To broaden the variability of PRTs in current peanut germplasms, three elite peanut cultivars were used to construct Ethyl methane sulfonate (EMS)-induced mutant libraries in this study. The optimal EMS treatment conditions for the three peanut varieties were determined. It was found that the median lethal dose (LD50) of EMS treatment varied greatly among different genotypes. Finally, the EMS-induced peanut… More >

  • Open Access

    ARTICLE

    Full-Length Transcriptome Analysis of Cultivated and Wild Tetraploid Peanut

    Danlei Song#, Xiaona Yu#, Yaoyao Li, Xianheng Wang, Xinyuan Cui, Tong Si, Xiaoxia Zou, Yuefu Wang, Minglun Wang, Xiaojun Zhang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 439-453, 2023, DOI:10.32604/phyton.2022.023165

    Abstract The high-quality genomes and large-scale full-length cDNA sequences of allotetraploid peanuts have been sequenced and released, which has accelerated the functional genomics and molecular breeding research of peanut. In order to understand the difference in the transcriptional levels of wild and cultivated peanuts. In this study, we integrated of second- and third-generation sequencing technologies to sequence full-length transcriptomes in peanut cv. Pingdu9616 and its putative ancestor Arachis monticola. The RNA extracted from six different tissues (i.e., roots, stems, leaves, flowers, needles and pods) were sampled at 20 days after flowering. A total of 31,764 and 33,981 high-quality transcripts were obtained… More >

  • Open Access

    ARTICLE

    Effects of Allelochemicals on Root Growth and Pod Yield in Response to Continuous Cropping Obstacle of Peanut

    Zhaohui Tang1,2,3, Feng Guo1,2,3, Li Cui1,2,3, Qingkai Li4, Jialei Zhang1,2,3, Jianguo Wang1,2,3, Sha Yang1,2,3, Jingjing Meng1,2,3, Xinguo Li1,2,3, Ping Liu2,3,5,*, Shubo Wan1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 17-34, 2023, DOI:10.32604/phyton.2022.022405

    Abstract

    Continuous cropping (CC) obstacle is a major threat in legume crops production; however, the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood. The current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals, p-hydroxybenzoic acid (H), cinnamic acid (C), phthalic acid (P), and their mixtures (M) on peanut root growth and productivity in response to CC obstacle. Treatment with H, C, P, and M significantly decreased the plant height, dry weight of the leaves and stems, number of branches, and length of the lateral stem compared with control. Exogenous… More >

  • Open Access

    ARTICLE

    Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature Peanut (Arachis hypogaea L.) Cultivars

    Abraham Lamboro1,*, Xiao Han1, Songnan Yang1, Xueying Li1, Dan Yao2, Baixing Song1, Jun Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2619-2631, 2022, DOI:10.32604/phyton.2022.021404

    Abstract Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied to evaluate shoot regeneration capacity whereas α-naphthalene acetic acid (NAA) combination with 6-benzylaminopurine (BAP), and 2, 4-dichlorophenoxyacetic acid (2, 4-D) with 6-benzylaminopurine were tested to optimize root induction for two peanut cultivars. The result showed combination (BAP with TDZ) was found to be effective in promoting shoot. The highest shoot regeneration frequency (93%) was obtained on a medium supplemented with 4 mg/L BAP and 0.5 mg/L TDZ while an average… More >

  • Open Access

    ARTICLE

    Steam Exploded Peanut Shell Fiber as the Filler in the Rigid Polyurethane Foams

    Zehui Ju1, Qian He1, Tianyi Zhan1, Haiyang Zhang1,*, Lin Sun1, Lu Hong1, Xinyi Shi2, Xiaoning Lu1,*

    Journal of Renewable Materials, Vol.7, No.11, pp. 1077-1091, 2019, DOI:10.32604/jrm.2019.07525

    Abstract In this study, steam exploded peanut shell fibers (SE-PSFs) were utilized to fabricate with rigid polyurethane foam (RPUF) in order to improve sound absorption performance and hydrothermal weather resistance. Optimized method of SE treatment, RPUF preparation and flame retardant treatment were selected to prepare SE-PSF/RPUF composites in this experiment. Physical and mechanical properties including density, water absorption capacity, thickness swelling rate, compressive strength, thermal conductivity and average sound absorption coefficient of SE-PSF/RPUF were investigated and compared with the control (PRUF). The results showed that the density, water absorption capacity, thickness swelling rate and thermal conductivity showed an increasing trend with… More >

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