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  • Open Access


    Morphological and Phylogenetic Resolution of Diplodia neojuniperi Emerging Diplodia Top Dieback of Pinus thunbergii Parl. in China

    Xiaolei Ding, Ruiwen Zhao, Sixi Lin, Jianren Ye*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2813-2825, 2022, DOI:10.32604/phyton.2022.024609

    Abstract In Bazhong City, Sichuan Province, China, top dieback symptoms were found on many pine trees (Pinus thunbergii Parl). The tips of old needles first turned grayish-green and then developed into brown bands in the field. Phylogenetic analysis of concatenated ITS and EF1-α indicated the pathogen of this dieback disease as Diplodia neojuniperi. Additionally, effects of temperature, pH and medium on the mycelial growth were also characterized. The most favorable temperature and pH level for mycelial growth are 25°C and 8, respectively. The optimal medium for mycelial growth is PDA medium. To our knowledge, this is the first report of D.… More >

  • Open Access


    Phylogenetic analysis and target gene prediction of miR477 gene family in grape


    BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718

    Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, respectively. The… More >

  • Open Access


    Complete genome sequence of two strawberry vein banding virus isolates from China


    BIOCELL, Vol.45, No.6, pp. 1695-1702, 2021, DOI:10.32604/biocell.2021.015250

    Abstract It was rarely reported about strawberry vein banding virus (SVBV) genome sequence in China and most countries worldwide. In this work, we determined the complete genome sequences of two SVBV isolates in China, designated SVBV-AH and SVBV-BJ, that were obtained from naturally infected strawberry samples from Anhui province and Beijing city of China, respectively. The complete genomes of SVBV-AH and SVBV-BJ were 7,862 nucleotides (nts) and 7,863 nts long, respectively, and both constituted with seven genes typical of the caulimoviruses. Alignment of complete nucleotide sequences showed that SVBV-AH and SVBV-BJ shared a significant nucleotide sequence identity of 97.7% of each… More >

  • Open Access


    Phylogenetic analysis of microRNA biomarkers for amyotrophic lateral sclerosis


    BIOCELL, Vol.45, No.3, pp. 547-561, 2021, DOI:10.32604/biocell.2021.014343

    Abstract Amyotrophic lateral sclerosis (ALS), also called Lou Gehrig’s disease, is an irreversible disease that is caused by the degeneration and death of motor neurons. Approximately 5–10% of cases are familial ALS (fALS), and the other cases are sporadic ALS (sALS). Gene mutations have been identified both in fALS and sALS patients. In this study, we discuss the four ALS-related genes, C9orf72, SOD1, FUS, and TARDBP, and review the microRNAs (miRNAs) that are associated with ALS and other neurological disorders from the literature. A phylogenetic analysis is used to explore potential miRNAs that can be taken into account when studying the… More >

  • Open Access


    Comparative Analyses and Phylogenetic Relationships between Cryptomeria fortunei and Related Species Based on Complete Chloroplast Genomes

    Yingting Zhang1,2,3, Junjie Yang1,2,3, Zhenhao Guo1,2,3, Jiaxing Mo1,2,3, Jiebing Cui1,2,3, Hailiang Hu1,2,3, Jin Xu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 957-986, 2020, DOI:10.32604/phyton.2020.011211

    Abstract Cryptomeria fortunei (Chinese cedar) is a highly adaptable woody species and one of the main forest plantation trees in subtropical high-altitude areas in China. However, there are few studies on its chloroplast (cp) genome. In this study, the complete cp genome of C. fortunei was sequenced and evaluated via comparative analyses with those of related species (formerly the Taxodiaceae) in Cupressaceae. The C. fortunei cp genome was 131,580 bp in length, and the GC content of the whole genome was 35.38%. It lost one relevant large inverted repeat and contained 114 unique genes, including 82 protein-coding genes, 28 tRNAs and… More >

  • Open Access


    The Chloroplast Genome of Cerasus Campanulata Diverges from Other Prunoideae Genomes

    Yuhao Weng1, Liming Zhu2, Yan Ma3, Hao Su1, Lu Lu1, Pengkai Wang1, Jinhui Chen1, Asif Ali1, Renhua Zheng4,*, Jisen Shi1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 375-384, 2020, DOI:10.32604/phyton.2020.08831

    Abstract Cerasus Campanulata is one of several species belonging to the Prunoideae focke, a subfamily of the flowering plant Rosaceae. We investigated the details of its chloroplast genome which may reveal its genus independent of morphological determination. Here, we determined the complete chloroplast (cp) genome sequence of C. campanulata and performed sequence analysis to reveal the presence of 18 forward repeats, 20 palindrome repeats, 2 complement repeats, 4 reverse repeats and 93 simple sequence repeats (SSRs). We additionally performed a comparative study of C. campanulata and seven other Prunoideae focke species. Then, maximum parsimony (MP) and maximum likelihood (ML) phylogenetic analyses… More >

  • Open Access


    Genome-Wide Identification of the F-box Gene Family and Expression Analysis under Drought and Salt Stress in Barley

    Lantian Zhang, Siyi Wang, Yuyu Chen, Mengyuan Dong, Yunxia Fang, Xian Zhang, Tao Tong, Ziling Zhang, Junjun Zheng, Dawei Xue*, Xiaoqin Zhang*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 229-251, 2020, DOI:10.32604/phyton.2020.10022

    Abstract The F-box protein-encoding gene family plays an essential role in plant stress resistance. In present study, 126 non-redundant F-box genes were identified in barley (Hordeum vulgare L., Hv). The corresponding proteins contained 165– 887 amino acid residues and all were amphiphilic, except 5 proteins. Phylogenetic analysis of F-box protein sequences in barley and stress-related F-box protein sequences in wheat and Arabidopsis thaliana (At) was used to classify barley F-box genes are divided into 9 subfamilies (A–I). A structure-based sequence alignment demonstrated that F-box proteins were highly conserved with a total of 10 conserved motifs. In total, 124 F-box genes were… More >

  • Open Access


    Chitinase, chitosanase, and antifungal activities from thermophilic streptomycetes isolated from compost

    González-Franco AC1, L Robles-Hernández1, JL Strap2

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 14-27, 2017, DOI:10.32604/phyton.2017.86.014

    Abstract The Streptomyces genus comprises a large and diverse group of bacteria, many of which are commercially exploited for the production of antibiotics and lytic enzymes. The thermophilic species are less studied than the predominant mesophilic species. However, the first ones are a potential source of thermostable bioactive products and enzymes with novel properties. In this study, two selected thermophilic streptomycetes were identified and their chitinolytic activities were evaluated. The identification of these two isolates was performed by microscopic morphology, partial 16S rDNA sequences, and its phylogenetic analysis. To study the chitinolytic activities of these isolates, the effects of colloidal chitin… More >

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