Deng JB¹, CB Ding¹, L Zhang¹, YH Zhou², RW Yang¹

Phyton-International Journal of Experimental Botany, Vol.80, pp. 181-188, 2011, DOI:10.32604/phyton.2011.80.181

Abstract Four isozymes, superoxide dismutase (SOD), polyphenol oxidase (PPO), malate dehydrogenase (MDH) and cytochrome oxidase (COD) were studied for identification of six herbal species (Curcuma L.). All the 37 study specimens produced a total of 168 polymorphism isozyme bands. The genetic distance coefficients (GS) varied from 0.08 to 0.54. The dendrogram, obtained according to the polymorphism isozyme bands by the UPGMA method with the software NTSYS–pc2.1, contributed to improve the resolution of phylogeny. From the dendrogram, it was possible to differentiate between the wild and cultivated specimens of C. longa, and within C. sichuanensis species. More >

Farshid GOLSHANI1, Barat Ali FAKHERI¹, Mahmood SOLOUKI¹, Nafiseh MAHDINEZHAD¹, Majid Reza KIANI FERIZ²

BIOCELL, Vol.43, No.3, pp. 225-232, 2019, DOI:10.32604/biocell.2019.07547

Abstract ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4 crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI). PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS). A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not already present in the scientific library. Nucleotide polymorphic sites were counted for ITS… More >