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  • Open Access

    ARTICLE

    Characterization of Endophytic Microorganisms of Rice (Oryza sativa L.) Potentials for Blast Disease Biocontrol and Plant Growth Promoting Agents

    Shugufta Parveen1, Fayaz A. Mohiddin2,*, M. Ashraf Bhat3, Zahoor Ahmed Baba4, Fehim Jeelani5, M. Anwar Bhat6, Sajad Un Nabi7, Burhan Hamid2, Saba Bandey8, Farhanaz Rasool9, Zakir Amin1, Ibrahim Al-Ashkar10,*, Muhammad Adnan11, Ayman El Sabagh12

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3021-3041, 2023, DOI:10.32604/phyton.2023.030921

    Abstract One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL−1) and siderophore (56.64%), was exhibited by Aspergillus flavus, and the highest HCN production was shown by Stenotrophomonas maltophilia (36.15 µM mL−1). In… More >

  • Open Access

    ARTICLE

    Structural characterization of four Rhododendron spp. chloroplast genomes and comparative analyses with other azaleas

    XIAOJUN ZHOU1,*, MENGXUE LIU1, LINLIN SONG2

    BIOCELL, Vol.47, No.3, pp. 657-668, 2023, DOI:10.32604/biocell.2023.026781

    Abstract Azalea is a general designation of Rhododendron in the Ericaceae family. Rhododendron not only has high ornamental value but also has application value in ecological protection, medicine, and scientific research. In this study, we used Illumina and PacBio sequencing to assemble and annotate the entire chloroplast genomes (cp genomes) of four Rhododendron species. The chloroplast genomes of R. concinnum, R. henanense subsp. lingbaoense, R. micranthum, and R. simsii were assembled into 207,236, 208,015, 207,233, and 206,912 bp, respectively. All chloroplast genomes contain eight rRNA genes, with either 88 or 89 protein-coding genes. The four cp genomes were compared and analyzed… More >

  • Open Access

    ARTICLE

    Genome-Wide Characterization of the Cellulose Synthase Gene Superfamily in Tea Plants (Camellia sinensis)

    Qianqian Li1, Qi Zhao1,2,*, Xinzhuan Yao1, Baohui Zhang1, Litang Lu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2163-2189, 2022, DOI:10.32604/phyton.2022.021530

    Abstract The cellulose synthase gene superfamily, including Cellulose synthase A (CesA) and cellulose synthase-like (Csl) gene families, is responsible for the synthesis of cellulose and hemicellulose, respectively. The CesA/Csl genes are vital for abiotic stress resistance and shoot tenderness regulation of tea plants (Camellia sinensis). However, the CesA/Csl gene family has not been extensively studied in tea plants. Here, we identified 53 CsCesA/Csl genes in tea plants. These genes were grouped into five subfamilies (CsCesA, CsCslB, CsCslD, CsCslE, CsCslG) based on the phylogenetic relationships with Arabidopsis and rice. The analysis of chromosome distribution, gene structure, protein domain and motif revealed that… More >

  • Open Access

    ARTICLE

    A New Record of Aspergillus vadensis (Ascomycota) Isolated from Soil in Yunnan Province, China

    Sadia Nadir1,2,3, Sehroon Khan1, Dhanushka N. Wanasinghe1,2, Saowaluck Tibpromma1,2, Shahid Iqbal1,2, Jianchu Xu1,2,*, Peter Mortimer1,2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1031-1039, 2021, DOI:10.32604/phyton.2021.012075

    Abstract During a survey of soil fungi in Yunnan Province, several isolates of Aspergillus were obtained. Based on morphology and molecular analyses of internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS), β-tubulin and calmodulin (CaM) genes sequences, two isolates were identified as Aspergillus vadensis (section Nigri). A phylogenetic tree, detailed descriptions, illustrations and scanned electron microscopy morphology are provided for the new isolates. This is the first record of A. vadensis from China. More >

  • Open Access

    ARTICLE

    Morphology, Phylogeny and Culture Characteristics of Ganoderma gibbosum Collected from Kunming, Yunnan Province, China

    Thatsanee Luangharn1,2,3,4, Samantha C. Karunarathna1,3, Peter E. Mortimer1,4,*, Kevin D. Hyde3,5, Jianchu Xu1,3,4,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 743-764, 2020, DOI:10.32604/phyton.2020.09690

    Abstract Ganoderma is a genus of medicinally and economically important mushrooms in the family Ganodermataceae. Ganoderma species are popular medicinal mushrooms and their health benefits are well-documented. Ganoderma is a cosmopolitan genus that is widely distributed in both tropical and temperate regions. This genus is characterized by its unique laccate or non-laccate species with double-walled basidiospores. Here, we report on eight collections of G. gibbosum collected during surveys in Kunming, Yunnan Province, China. The specimens are described and illustrated based on macro- and micro-morphological characteristics. Total DNA of the eight G. gibbosum strains were extracted using the Biospin Fungal Extraction Kit… More >

  • Open Access

    ARTICLE

    Sexual Morph of Furcasterigmium furcatum (Plectosphaerellaceae) from Magnolia liliifera Collected in Northern Thailand

    Jutamart Monkai1, Ruvishika S. Jayawardena1,2, Sajeewa S. N. Maharachchikumbura3,*, Kevin D. Hyde1

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 765-777, 2020, DOI:10.32604/phyton.2020.09720

    Abstract We isolated an interesting fungus from dead leaves of Magnolia liliifera collected from Chiang Mai, Thailand. The novel strain is related to Plectosphaerellaceae based on the morphology of its asexual morph and the analysis of sequence data. Phylogenetic analyses using a combined gene analysis of LSU and ITS sequence data showed that this strain is clustered in the same clade with Furcasterigmium furcatum with high statistical support. The new strains produced the asexual morph in culture which is morphologically similar to F. furcatum. Thus, we identified this strain as the sexual morph of F. furcatum. This is the first record… More >

  • Open Access

    ABSTRACT

  • Open Access

    ARTICLE

    A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata

    ISRAEL A. VEGA*1,2, FEDERICO A. DELLAGNOLA1, JORGE A. HURST3, MARTÍN S. GODOY1 AND ALFREDO CASTRO-VAZQUEZ1,2

    BIOCELL, Vol.36, No.2, pp. 47-55, 2012, DOI:10.32604/biocell.2012.36.047

    Abstract Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (CI , CII and CIII ), were studied by positive ion fast atom bombardment-mass spectrometry (FAB–MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored CI fraction; (2) a mixture of pheophorbides a and b in the major green fraction, CII; and (3) a modified pheophorbide a in the… More >

  • Open Access

    ARTICLE

    Comparative fine structure of the epididymal spermatozoa from three korean shrews with considerations on their phylogenetic relationships

    SOON-JEONG JEONG1, JOO-CHEOL PARK1, HEUNG-JOONG KIM2, CHUN SIK BAE3, MYUNG-HEE YOON4, DO-SEON LIM5, MOON-JIN JEONG1

    BIOCELL, Vol.30, No.2, pp. 279-286, 2006, DOI:10.32604/biocell.2006.30.279

    Abstract This study examined the fine structures of epididymal spermatozoa on the lesser white-toothed shrew (Crocidura suaveolens), the Japanese white-toothed shrew (C. dsinezumi) and the big white-toothed shrew (C. lasiura) belonging to the subfamily Crocidurinae living in Korea.
    In the spermatozoa of C. suaveolens, the head has a large acrosome, a smooth inner acrosomal membrane and a wavy, finger-like, electron-dense apical body. The neck has a solid proximal centriole that is filled with electron-dense material. These results showed the spermatozoa of C. suaveolens possess the characteristics of both Crocidurinae and Soricinae. In C. dsinezumi and C. lasiura, the head has… More >

  • Open Access

    ARTICLE

    Karyotype description of Pomacea patula catemacensis (Caenogastropoda, Ampullariidae), with an assessment of the taxonomic status of Pomacea patula

    MARÍA ESTHER DIUPOTEX-CHONG1, NÉSTOR J. CAZZANIGA2, ALEJANDRA HERNÁNDEZ-SANTOYO3, JOSÉ MIGUEL BETANCOURT-RULE4

    BIOCELL, Vol.28, No.3, pp. 279-285, 2004, DOI:10.32604/biocell.2004.28.279

    Abstract Mitotic chromosomes of the freshwater snail Pomacea patula catemacensis (Baker 1922) were analyzed on gill tissue of specimens from the type locality (Lake Catemaco, Mexico). The diploid number of chromosomes is 2n = 26, including nine metacentric and four submetacentric pairs; therefore, the fundamental number is FN = 52. No sex chromosomes could be identified. The same chromosome number and morphology were already reported for P. flagellata, i.e., the other species of the genus living in Mexico. The basic haploid number for family Ampullariidae was reported to be n° =°14 in the literature; so, its reduction to n° =°13 is… More >

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