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  • Open Access

    ARTICLE

    Phytochemical Analysis, Estimation of Quercetin, and in Vitro Anti-Diabetic Potential of Stevia Leaves Samples Procured from Two Geographical Origins

    Kuntal Das1, Syed Mohammed Basheeruddin Asdaq2,*, M. Saifulla Khan1,*, Sravani Singirikonda1, Abdulhakeem S. Alamri3,4, Walaa F. Alsanie3,4, Majid Alhomrani3,4, Sreeharsha Nagaraja5,6, Katharigatta N. Venugopala5,7

    Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2349-2365, 2022, DOI:10.32604/phyton.2022.022379

    Abstract The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert. (SRB) leaves collected from two different geographical sources. SRB leaves were collected from Bangalore, Karnataka, India, and Reduit, Mauritius. Extracts were prepared using ethanol and aqueous solvents. Proximate analysis was used to evaluate moisture content, ash values, crude fibers, and extractive values. Following that, preliminary phytochemical screening was done on both ethanol leaves extracts, and subsequently total flavonoid content was determined. In addition, TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence of flavonoid components in… More >

  • Open Access

    ARTICLE

    Assessment of Phytochemical Analysis, Nutritional Composition and Antimicrobial Activity of Moringa oleifera

    Naima Asghar1, Abida Aziz1, Muhammad Farooq Azhar2 , Mohamed El-Sharnouby3, Uzma Irfan1, Iqra Rafiq1, Husnain Farooq4,5,*, Muhammad Ishaq Asif Rehmani6, Mohamed I. Sakran7, A. Altalhi8, Fahad M. Alzuaibr9, Ayman El Sabagh10,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1817-1829, 2022, DOI:10.32604/phyton.2022.020790

    Abstract Moringa oleifera is a miracle plant rich in nutrients, antioxidants, and antibiotic properties. Present study was designed to evaluate various biochemical attributes of leaves and flowers of M. oleifera. Plant parts (leaves, flowers) of M. oleifera, collected from different roadsides of Multan district, Punjab, Pakistan, were used as experimental material. Result indicates that alkaloids, saponin, carbohydrates, fats, and protein had a high value in the aqueous extract of both leaves and flowers of M. oleifera. Whereas phenol content was high in methanolic leaves extract and the phenol contents were high in aqueous extract of flowers. The extract yield of M.… More >

  • Open Access

    ARTICLE

    Embryo and Endosperm Phytochemicals from Polyembryonic Maize Kernels and Their Relationship with Seed Germination

    J. David García-Ortíz1, Rebeca González-Centeno1, María Alejandra Torres-Tapia2, J. A. Ascacio-Valdés1, José Espinoza-Velázquez2, Raúl Rodríguez-Herrera1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 929-941, 2022, DOI:10.32604/phyton.2022.018368

    Abstract Because of the growing worldwide demand for maize grain, new alternatives have been sought for breeding of this cereal, e.g., development of polyembryonic varieties, which agronomic performance could positively impact the grain yield per unit area, and nutritional quality. The objectives of this study were to (1) determine the phytochemicals present in the embryo and endosperm of grain from maize families with high, low, and null polyembryony frequency, which were planted at different locations, and (2) state the relationship between these compounds and seed germination. The extracted phytochemicals from corn were identified by HPLC-MS. The results showed that the genotype… More >

  • Open Access

    ARTICLE

    Anti-lipid droplets accumulation effect of Annona montana (mountain soursop) leaves extract on differentiation of preadipocytes

    IVY LEUNG1, MARIA-LUISA VEISAGA2, MARGARITA ESPINAL1, WEI ZHANG1, ROBERT BARNUM1, MANUEL ALEJANDRO BARBIERI1,2,3,4,*

    BIOCELL, Vol.46, No.3, pp. 567-578, 2022, DOI:10.32604/biocell.2022.014009

    Abstract The Annona genus is a member of Annonaceae, one of the largest families of plants across tropical and sub-tropical regions. This family has been used in several ethnomedicinal practices to treat a multitude of human diseases. However, the molecular mechanism underlying its effect on the lipid droplet formation and on the expression of adipogenic markers of this plant remain to be investigated. In this study, we examined whether the extracts from the aerial part of Annona montana affect in vitro differentiation of preadipocytes. For our investigations, both mouse embryo fibroblast 3T3-L1 and normal human primary subcutaneous preadipocytes were incubated with… More >

  • Open Access

    ARTICLE

    Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves

    Olha Mykhailenko1,*, Ivan Bezruk1, Victoriia Volochai2, Volodymyr Mishchenko3, Liudas Ivanauskas4, Victoriya Georgiyants1

    Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 207-221, 2022, DOI:10.32604/phyton.2022.016458

    Abstract The numerous studies indicate leaves of plants are a rich source of bioactive compounds that can be a valuable source of compounds used in the pharmaceutical and cosmetic industries. Aim of this study was to investigate the chemical composition and the antioxidant property of Crocus speciosus leaves. Primary phytochemical screening of C. speciosus leaves revealed the presence of some following compound categories such as phenolic compounds, aminoacids, saponins, proteins, tannins, triterpenoids, glycosides, polysaccharides. The total flavonoids and phenolic compounds content were determined spectrophotometrically and by HPLC-DAD and HPLC-MS. Antiradical activity was determined by ABTS radical-cation scavenging method, spectrophotometrically. The total… More >

  • Open Access

    ARTICLE

    Tentative Identification of Phytochemicals and Antioxidant Activities during Fruit-Ripening on Chamaedorea radicalis Mart.

    Gerardo Montelongo-Ruíz1, Yolanda del Rocio Moreno-Ramírez1, María Cruz Juárez-Aragón1, Nohemí Niño-García2, Reyna Ivonne Torres-Acosta2, Torres-Castillo Jorge Ariel1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 361-373, 2020, DOI:10.32604/phyton.2020.08499

    Abstract This work aims to determine the phytochemical characterization of the pericarp of Chamaedorea radicalis Mart. fruit as a non-timber product with potential to obtain phytochemicals with potential applications in the industry. Fruit from C. radicalis were grouped in four ripening stages named as S1, S2, S3 and S4, according to maturity; S1 the most unripe stage and S4 the completely ripe stage. Determinations of total phenolic compounds, free radical scavenging activities and total flavonoid contents using spectrophotometric methods were done. Also, the tentative identification of phytochemicals during fruit ripening was done using UPLC-MS-MS. Total phenolic compound (TPC) content ranged from… More >

  • Open Access

    ARTICLE

    Phytochemical quality of tomato Saladette produced with organic substrates under greenhouse conditions

    González B ML1, M Fortis H1, P Preciado R1, MA Segura C1, E Salazar Sosa1, JL García H2, JR Esparza R3

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 71-78, 2016, DOI:10.32604/phyton.2016.85.071

    Abstract The aim of the current study was to evaluate yield and phytochemical quality of tomato fruits produced using organic substrates under greenhouse conditions. The applied treatments (organic substrates) were: T1 (80% River sand + 20% Vermicompost; T2 (90% River sand + 10% Compost); T3 Steiner Solution (80% River sand + 20% Perlite); T4 (80% River sand + 5% Soil + 15% Vermicompost); T5 (85% River sand + 15% Treated manure), and T6 (80% River sand + 5% Soil + 15% Treated manure). We used a totally randomized experimental design, with four replicate treatments. Evaluated variables were: yield, relative content of… More >

  • Open Access

    ARTICLE

    Phytochemical and pharmacological studies on Mikania micrantha H.B.K. (Asteraceae)

    Pérez-Amador1 MC, V Muñoz Ocotero1, R Ibarra Balcazar1, F García Jiménez2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 77-80, 2010, DOI:10.32604/phyton.2010.79.077

    Abstract Asteraceae is one of the largest families of Angiospermae, and contains the Mikania genus. Several species of this family contain polyacetylenic and thiophenic compounds which are used as taxonomic markers. Also, their phototoxic activity acts as plant defense mechanism. In this paper we report the absence of these compounds in Mikania micrantha. We also describe the composition of the volatile oil from the seeds and inflorescence in the species. Linalool and α-pinene were the main components of this M. micrantha’s essential oil. This study tested the ability of extracts of Mikania micrantha to inhibit the mouse ear inflammation in response… More >

  • Open Access

    ARTICLE

    In vitro antiviral activity and phytochemical screen in the extracts of peels from four species of tropical fruits collected in Merida Yucatan, Mexico

    Chel-Guerrero LD1, R Gómez-Cansino2, SL Gúzman-Gutierrez3, MG Campos-Lara4, E Saury-Duch1, F Díaz de León Sánchez5, R Reyes-Chilpa6, JA Mendoza-Espinoza7

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 68-71, 2018, DOI:10.32604/phyton.2018.87.068

    Abstract The objective of this study was to investigate extracts from the peels of Annona squamosa L. (purple sugar apple), Annona reticulata L. (custard apple), Chrysophyllum cainito L. (green star apple), and Melicoccus bijugatus Jacq. (mamoncillo), as potential sources of anti-HIV-1 agents. Methanolic extracts from the peels of the aforementioned tropical fruits were obtained by maceration. Antiviral activity was evaluated through HIV-1 RT inhibition by extracts, using a non-radioactive immuno/colorimetric assay (Lenti RT Activity Assay, Cavidi Tech). Assay was performed according to the protocol provided by the manufacturer. Extracts from the peels of A. squamosa, A. reticulata, and C. cainito showed… More >

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