Luksamee Vittaya^1,*, Chakhriya Chalad¹, Sittichoke Janyong², Nararak Leesakul³

Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 4091-4115, 2025, DOI:10.32604/phyton.2025.074891 - 29 December 2025

Abstract Calophyllum inophyllum is a tropical plant that could have useful medicinal properties for pharmaceutical and cosmetic applications. The present study extracted the flower, fruit, leaf, twig, and bark of the plant by maceration in different organic solvents. The correlation between bioactive compounds and their biological activities was investigated, with emphasis on their therapeutic relevance through in silico pharmacokinetic predictions using SwissADME. Qualitative and quantitative analyses were conducted to determine the total phenolic, flavonoid, and saponin contents of the extracts. Spectral analysis of the extracts revealed –OH, C=O, C=C, and C–H functional groups. The antioxidant activity of the… More > Graphic Abstract

Oleh Koshovyi¹, Amal D. Premarathna², Rando Tuvikene², Valdas Jakštas^3,4, Vaidotas Žvikas³, Yurii Hrytsyk⁵, Lyubov Grytsyk⁵, Andriy Grytsyk⁵, Jaan Liira⁶, Ain Raal^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 3991-4019, 2025, DOI:10.32604/phyton.2025.074153 - 29 December 2025

Abstract Gilia capitata Sims (Polemoniaceae) is an annual herbaceous plant widely distributed in western North America and cultivated as an ornamental flower. The detailed chemical composition and pharmacological properties of this plant have not been previously reported. This study aimed to investigate the chemical composition and pharmacological activity of aqueous-ethanolic extracts derived from different plant parts (herb, flowers, stems, roots, and fruits). HPLC analysis identified 12 phenolic compounds and 16 amino acids in the aqueous-ethanolic extracts obtained from these plant parts. The biological effects of the extracts were evaluated in vitro, including cytotoxicity, anti-inflammatory response, and wound-healing potential.… More >

Elizabeth Martinez¹, Ana K. Escalera-Ordaz¹, Francisco J. Espinosa-García², Yolanda M. García-Rodríguez², Rafael Ariza-Flores³, Javier Ponce-Saavedra⁴, Patricio Apáez-Barrios⁵, Héctor Guillén-Andrade^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 4155-4171, 2025, DOI:10.32604/phyton.2025.073438 - 29 December 2025

Abstract Volatile organic compounds (VOCs) play an important role in plant survival and adaptation. They contribute to defense against pests and pathogens, tolerance to abiotic stress, and the mediation of essential ecological interactions such as pollination and attraction of dispersal agents. The complex mixture of VOCs produced by an organism, known as volatilome, varies across species, populations, and individuals, making VOCs a major factor in crop diversification and adaptation. In this context, characterizing the volatilome of crop genotypes can provide insight into their ecological associations and potential relationships with agronomic traits. In this study, the volatilome… More >

Yousra Hammouti^1,2,*, François Mesnard², Oussama Khibech³, Mohamed Taibi¹, Yousra Belbachir³, El Hassania Loukili⁴, Reda Bellaouchi⁵, Abdeslam Asehraou⁵, Mohamed Addi^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3563-3583, 2025, DOI:10.32604/phyton.2025.071139 - 01 December 2025

Abstract Centaurium erythraea Rafn (“Gosset El Haya”) has long been prized in North African folk medicine, yet Moroccan chemobiological data remain scarce. Ethanol extracts of northern Moroccan aerial parts were profiled by high-performance liquid chromatography (HPLC) and found rich in phenolics, dominated by 4-hydroxybenzoic acid (57.8%) and naringin (10.3%). The extract exhibited strong antioxidant power in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, with a half-maximal inhibitory concentration (IC₅₀) of ≈74 µg mL⁻¹, and a total antioxidant capacity (TAC) of ≈201 µg mL⁻¹ and selective antimicrobial activity, sharply inhibiting Aspergillus niger, Penicillium digitatum, and Rhodotorula glutinis while sparing Staphylococcus aureus. In-silico absorption, distribution, metabolism,… More >

Sheikh Showkat Ahmad¹, Chandni Garg¹, Dalia Fouad², Islam Abdulrahim Alredah³, Sandeep Kaur⁴, Satwinderjeet Kaur^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3401-3426, 2025, DOI:10.32604/phyton.2025.067984 - 01 December 2025

Abstract The Tragopogon dubius is traditionally used to treat many ailments, consumed as a vegetable, and utilized as fodder for livestock. Tragopogon dubius, found in the Kashmir Himalayas, is the least explored for its bioactivity properties and has a unique geographical location. This study is the first attempt to investigate the antioxidant, anticancer, and genoprotective properties of the aqueous extracts from the leaves (AQ-TrDL) and stems (AQ-TrDS) of this plant. AQ-TrDL and AQ-TrDS demonstrated significant amounts of phenolic and flavonoid contents. GC-HRMS identified various phytochemicals belonging to different classes, like carboxylic acids, fatty acid derivatives, phenols, and triterpenoids.… More >

Sy-Yu Shiau^*, Shuting Ni, Yanli Yu, Songling Cai, Wenbo Huang

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3047-3060, 2025, DOI:10.32604/phyton.2025.070289 - 29 October 2025

Abstract Consumers are increasingly demanding natural colorants that are safe and offer health benefits. In addition to their ornamental characteristics, Kanzan cherry (KC) blossoms present a promising source of red-hued natural colorants and functional bioactive substances. This research utilized distilled water to extract KC petals (KCP) and their ground powders (KCPP) under varying temperatures (30°C–90°C) and times (30–180 min). The total monomeric anthocyanins (TMAC) and total phenolics (TPC) in the extracts were evaluated via the pH differential and Folin–Ciocalteu methods. Antioxidant capacities were assessed by DPPH free radical scavenging ability and reducing power. Results indicated that… More >

Oscar Sariñana-Aldaco¹, Carmen Alicia Ayala-Contreras¹, Susana González-Morales², Gregorio Cadenas-Pliego³, Marissa Pérez-Alvarez³, América Berenice Morales-Díaz⁴, Dámaris Leopoldina Ojeda-Barrios⁵, José Gerardo Uresti-Porras⁶, Adalberto Benavides-Mendoza^7,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2131-2153, 2025, DOI:10.32604/phyton.2025.067577 - 31 July 2025

Abstract Selenium (Se) is a nutrient that is considered beneficial for plants, because its improvement in growth, yield and quality helps plants to mitigate stress. The objective of this research was to evaluate the application of sodium selenite (Na₂SeO₃), nanoparticles (SeNPs) and microparticles (SeMPs) of Se in cucumber seedlings, via two experiments: one with seed priming and the other with foliar application of Se materials. The doses used were: 0, 0.1, 0.5, 1.0, 1.5 and 3.0 mg · L⁻¹, for each form of Se and for each form of application. Treatment 0 consisted of the application of… More >

Bismillah Mubeen^1,2,#, Ammarah Hasnain^2,3,#,*, Syed Atif Hasan Naqvi⁴, Fahad Hakim⁵, Syed Sheharyar Hassan Naqvi⁶, Muhammad Zeeshan Hassan⁴, Muhammad Umer Iqbal⁷, Mahmoud Moustafa⁸, Mohammed O. Alshaharni⁸, Mingzheng Duan^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 1941-1971, 2025, DOI:10.32604/phyton.2025.064056 - 31 July 2025

Abstract Plants have long served as a cornerstone for drug discovery, offering a vast repertoire of bioactive compounds with proven efficacy in combating oxidative stress, a pivotal driver of chronic diseases such as cancer, diabetes, cardiovascular disorders, and neurodegenerative conditions. This review synthesizes current knowledge on plant-derived antioxidants, emphasizing their mechanisms, therapeutic potential, and quantitative efficacy validated through standardized assays. Key phytochemicals, including polyphenols, carotenoids, flavonoids, and terpenoids, neutralize reactive oxygen species (ROS) through radical scavenging, enzyme modulation, and gene regulation. For instance, lutein, a carotenoid found in leafy greens, demonstrates potent antioxidant activity with IC₅₀ values… More >

Deen Bandhu Dimri¹, Manisha Nigam^1,*, Naresh Singh¹, Shilpa Semwal¹, Marcello Iriti^2,3,*, Abhay Prakash Mishra⁴

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 1007-1027, 2025, DOI:10.32604/phyton.2025.061270 - 31 March 2025

Abstract Cornus capitata, commonly known as the Himalayan strawberry, is a dogwood species from the family Cornaceae. Dogwood plants have been used extensively to practice conventional medicine due to their biological activities; however, the mechanisms behind these activities, particularly in C. capitata, remain largely unexplored. This study focuses on the preliminary phytochemical screening, antioxidant activity, anti-diabetic potential, and antimicrobial profiling of different extracts (i.e., leaves, bark, and fruit) from C. capitata to evaluate its potential pharmaceutical and medicinal applications. The in-vitro antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, Ferric-reducing antioxidant power (FRAP) assay, and Superoxide Anion Scavenging assay.… More > Graphic Abstract

Oleh Koshovyi^1,2,*, Janne Sepp¹, Valdas Jakštas³, Vaidotas Žvikas³, Karina Tolmacheva⁴, Igor Kireyev⁴, Jyrki Heinämäki¹, Ain Raal^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2909-2925, 2024, DOI:10.32604/phyton.2024.056536 - 30 November 2024

Abstract Pineappleweed (Matricaria discoidea DC., Asteraceae) herb is an essential oil containing raw material with spasmolytic and anti-inflammatory activity. It is also rich in phenolics, which may be used in pharmaceutical practice. This study aimed to investigate the phenolic and amino acid composition and the hyporific and analgesic effects of the M. discoidea aqueous-ethanolic extract and its amino acid modifications. In addition, we developed a polyethylene oxide gel formulation with M. discoidea extracts for the 3D-printed oral solid dosage preparations. In M. discoidea extracts, 16 phenolic substances and 14 amino acids were established. The extract and its amino acid preparations More >