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  • Open Access

    ARTICLE

    Diversity patterns of climbing plants and vascular epiphytes in the Valdivian rain forest of South America: a synthesis between 2000 and 2010

    Pincheira-Ulbrich J

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 9-18, 2011, DOI:10.32604/phyton.2011.80.009

    Abstract Knowledge about the diversity patterns of vascular epiphytes and vines in the Valdivian rainforest of South America between 2000 and 2010 was systematized. The method was based on the review of publications available in four electronic databases using keywords. The results showed 12 studies conducted in Chile and two in Argentina, mostly in primary forests within protected areas. Research was carried out essentially at the habitat level, although there were one study at a landscape level and two reviews at a regional scale. The samples used came from ground-based observations and only two studies accessed… More >

  • Open Access

    ARTICLE

    Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants

    SILVIA VILA, ANA GONZALEZ, HEBE REY AND LUIS MROGINSKI*

    BIOCELL, Vol.34, No.1, pp. 7-14, 2010, DOI:10.32604/biocell.2010.34.007

    Abstract Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined More >

  • Open Access

    ARTICLE

    Plant regeneration of Mullein Nightshade (Solanum donianum Walp.) from leaf explants

    O’Connor-Sánchez1 A, AV Domínguez-May1, MA Keb-Llanes1, YJ Peña-Ramírez2, VA Herrera-Valencia1

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 25-29, 2010, DOI:10.32604/phyton.2010.79.025

    Abstract A reliable protocol for plant regeneration of Mullein Nightshade (Solanum donianum Walp.) was developed from in vitro leaf explants. They were cultured on Murashige and Skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. The formulation that originated most shoots per explant was 8.5 μM zeatin riboside in absence of 1-naphthalene acetic acid. Explants with shoots were transferred to Murashige and Skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. Elongated shoots were individualized, and they rooted readily in half-strength Murashige and Skoog More >

  • Open Access

    ARTICLE

    Records of Sclerotinia minor infecting cultivated and invasive plants in the southern Pampas and northern Patagonia, Argentina, including comparative studies with Sclerotinia sclerotiorum

    Kiehr M1, R Delhey1, J Lusto2, PJ Paoloni3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 199-202, 2010, DOI:10.32604/phyton.2010.79.199

    Abstract A report is given on host plants, distribution, damage and importance of Sclerotinia minor in the southern Pampas and northern Patagonia regions (Argentina). Spontaneous infections were recorded on seven plant species. Sonchus oleraceus and Eruca vesicaria are new hosts for S. minor; Cichorium intybus and Zinnia elegans are new hosts for Argentina. This fungus causes important damage on Helianthus annuus and Lactuca sativa, and it occasionally affects C. intybus and Solanum lycopersicum. Comparative in vitro and in planta studies were carried out with S. minor and S. sclerotiorum isolates obtained from sunflower. More >

  • Open Access

    ARTICLE

    Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture

    MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

    BIOCELL, Vol.33, No.3, pp. 179-186, 2009, DOI:10.32604/biocell.2009.33.179

    Abstract With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of More >

  • Open Access

    ARTICLE

    Sclerotinia sclerotiorum infecting cultivated and invasive plants in the southern Pampas and northern Patagonia, Argentina

    Delhey R1, M Kiehr1, MI Allievi1, J Lusto2, S Frayssinet1, B Sidoti Hartmann3, I Kröger1, PJ Paoloni4, D Zappacosta1, A Servera3

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 111-115, 2009, DOI:10.32604/phyton.2009.78.111

    Abstract A report is given on plant hosts, distribution, damage and importance of Sclerotinia sclerotiorum in the southern Pampas region and northern Patagonia (Argentina). Spontaneous infections were recorded on 25 plant species, including oil crops, vegetables, and ornamental and invasive plants. Carduus thoermeri, Xanthium cavanillesii, Marrubium vulgare and Ranunculus repens are new hosts of S. sclerotiorum. The records on Helianthus tuberosus, Diplotaxis tenuifolia and Cucurbita pepo are new to Argentina. Sclerotinia sclerotiorum causes important damage on Helianthus annuus, Glycine max, Apium graveolens, Foeniculum vulgare and Lactuca sativa. S. sclerotiorum is also reported on Daucus carota, Petroselinum crispum, Brassica oleracea, B. rapa ssp. pekinensis, Cucumis melo, Cucurbita maxima, Cucurbita moschata, Cucurbita pepo, Phaseolus vulgaris, More >

  • Open Access

    ARTICLE

    Molecular and cultural analysis of seasonal actinomycetes in soils from Artemisia tridentata habitat

    Gonzalez-Franco AC1, L Robles-Hernandez1, A Nuñez-Barrios1, JL Strap2, DL Crawford3

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 83-90, 2009, DOI:10.32604/phyton.2009.78.083

    Abstract In order to understand the temporal dynamics of actinomycete communities of the rhizosphere of the desert plant Artemisia tridentata (sagebrush), two complementary methods were used. They were: (1) 16S rDNA-based PCR coupled with denaturing gradient gel electrophoresis (DGGE), and (2) an agar plate enumeration methodology in which three different media were used to quantify total bacteria, actinomycetes, and fungi. The objective of this research were: (1) to obtain a comprehensive picture of the structure of actinomycete populations, and (2) their dynamics in the rhizosphere of young and old sagebrush plants during two distinct seasons. PCR-DGGE analysis… More >

  • Open Access

    ARTICLE

    Heavy metal accumulation in Pelargonium hortorum: Effects on growth and development

    Orroño DI, RS Lavado*

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 75-82, 2009, DOI:10.32604/phyton.2009.78.075

    Abstract Ornamental plants have been proposed for growing in heavy metal (HM) contaminated soils, and also for phytoremediation. We evaluated (1) biomass production and (2) HM accumulation in Pelargonium hortorum. Plants were grown for 16 weeks on HM (cadmium, chromium, copper, lead, nickel and zinc) enriched soils. Treatments were i) control, non-enriched soil, ii) medium concentration treatment, and iii) high concentration treatment. Four destructive harvests were carried out, and roots, stems, leaves, and flowers were analyzed each time. Concentrations of HM were determined using ICP. Significant reductions in biomass production were observed in HM-enriched soils compared with More >

  • Open Access

    ARTICLE

    Effect of cell size and cytokinins on growth of petunia plants

    Lagoutte1 S, M Divo de Sesar2, F V ilella3

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 31-36, 2009, DOI:10.32604/phyton.2009.78.031

    Abstract Annual floral plant crops are produced in cells or pots of various sizes. Varying container size alters the volume which plant roots can explore, which in turn affect plant growth. This work assesed the effect of the initial container size on growth of Petunia x híbrida cv Dream Mix, supplemented with cytokinins after potting. Seedlings obtained in trays of 90 or 288 cells, were transplanted to 1l pots. They were then supplemented with 6-bencyladenine (BA). The assay consisted of 4 treatments: two container sizes by two BA conditions (with and without). Cell size conditioned growth above… More >

  • Open Access

    ARTICLE

    Nitrogen metabolism in roots and leaves of green bean plants exposed to different phosphorus doses

    Sánchez E1, G Ávila-Quezada1, AA Gardea1, E Muñoz1, JM Ruiz2, L Romero2

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 11-16, 2009, DOI:10.32604/phyton.2009.78.011

    Abstract The objective of this work was to determine the effect of different P doses on nitrogen assimilation in roots and leaves of green beans plants (Phaseolus vulgaris L. cv. Strike). Phosphorus was applied in the nutrient solution as H3PO4, at the nutrient doses of: P1 = 0.40 mM; P2 = 0.80 mM; P3 = 1.60 mM; P4 = 3.20 mM; P5 = 4.80 mM, and P6 = 6.40 mM P. Our results indicate that both P toxicity and deficiency gave similar responses to N assimilation. Phosphorus and NO3 - interacted on the absorption and translocation processes affecting More >

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