Jin G^1,2, XM Tang³, Y Niu⁴, XY Huang², T Chen², Q Huang², J Zhang², RY Zhou¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 137-142, 2017, DOI:10.32604/phyton.2017.86.137

Abstract In plants, the mitochondrial NADH dehydrogenase (complex I) is a large protein complex transferring electrons to ubiquinone. For the nine chondriogenes encoding complex I subunits (nad1, nad2, nad3, nad4, nad4L, nad5, nad6, nad7, and nad9), an efficient strategy for the cloning of complete coding sequences (CDSs) is important. Specific orthologous portions of untranslated regions (UTRs) were found based on multiple sequence alignments of chondriogene orthologues encoding complex I subunits in plant species. Based on the conservation of partial UTRs, a one-step PCR strategy was conceived for the cloning of CDSs of the nine chondriogene orthologues. Using this strategy, the five… More >

VIVIANE DAL-SOUTO FRESCURA¹, ANDRIELLE WOUTERS KUHN¹, HAYWOOD DAIL LAUGHINGHOUSE IV², JUÇARA TEREZINHA PARANHOS¹, SOLANGE BOSIO TEDESCO¹

BIOCELL, Vol.37, No.2, pp. 23-28, 2013, DOI:10.32604/biocell.2013.37.023

Abstract Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared… More >

MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO S.TRIPPI

BIOCELL, Vol.26, No.2, pp. 217-223, 2002, DOI:10.32604/biocell.2002.26.217

Abstract Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which “model”… More >

L.A. CARO¹, P.A. POLCI¹ , L.I. LINDSTRÖM¹ , C.V. ECHENIQUE^1,2, L.F. HERNÁNDEZ^1,3

BIOCELL, Vol.26, No.1, pp. 25-33, 2002, DOI:10.32604/biocell.2002.26.025

Abstract Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 – 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated… More >

María P BENAVIDES¹, Susana M GALLEGO¹, Facundo RAMOS ARTUSO², Mariana CHECOVICH³, Andrea GALATRO^*

BIOCELL, Vol.40, No.1, pp. 1-6, 2016, DOI:10.32604/biocell.2016.40.001

Abstract Plants are exposed to environmental stress, in natural and agricultural conditions.Nitric oxide (NO), a small gaseous molecule which plays important roles in plants, has been involved in many physiological processes, and emerged as an important endogenous signaling molecule in the adaptation of plants to biotic and abiotic stress. NO is produced from a variety of enzymatic and non enzymatic sources, which are not yet fully understood. Also, NO and reactive nitrogen species (RNS) can produce posttranslational modifications affecting protein function. Nitrate reductase, a key enzyme in the nitrogen metabolism, is a proposed source of NO in plants which could be… More >

Wang XG¹, Wuyunna^1*, CA Busso², YT Song¹, FJ Zhang¹, GW Huo¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 123-132, 2018, DOI:10.32604/phyton.2018.87.123

Abstract Chemical elements, such as carbon (C), nitrogen (N) and phosphorus (P), are major limiting nutrients in arid and semiarid grasslands and their stoichiometry (C:N:P) is a very important ratio to determine. In addition, it is critical to understand how plant stoichiometry responds to multiple environmental factors at the species level. In this study, we conducted a greenhouse experiment to investigate the effects of salt stress (4 g NaCl/kg soil), drought (35% of the soil water holding capacity) and N addition (10 g N/m²), as well as their interactions, on C, N and P concentrations and C:N:P ratios in two dominant… More >

Mirza Hasanuzzaman^1,*, Aditya Banerjee², M. H. M. Borhannuddin Bhuyan^3,4, Aryadeep Roychoudhury^2,*, Jubayer Al Mahmud⁵ and Masayuki Fujita³

Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 185-221, 2019, DOI:10.32604/phyton.2019.07559

Abstract In the era of climate change, abiotic stresses (e.g., salinity, drought, extreme temperature, flooding, metal/metalloid(s), UV radiation, ozone, etc.) are considered as one of the most complex environmental constraints that restricts crop production worldwide. Introduction of stress-tolerant crop cultivars is the most auspicious way of surviving this constraint, and to produce these types of tolerant crops. Several bioengineering mechanisms involved in stress signaling are being adopted in this regard. One example of this kind of manipulation is the osmotic adjustment. The quarternary ammonium compound glycinebetaine (GB), also originally referred to as betaine is a methylated glycine derivative. Among the betaines,… More >

Ibrahim M. Abdelsalam¹, Mostafa Elshobary^1,3,*, Mohamed M. Eladawy¹, Mohammed Nagah²

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 69-90, 2019, DOI:10.32604/phyton.2019.06831

Abstract Heavy metal contamination of land and freshwater resources is a serious concern worldwide. It adversely affects the health of animals, plants and humans. Therefore, remediation of toxic heavy metals must be highly considered. Unlike other techniques, phytoremediation is a holistic technology and can be used in large scale for soil remediation as it is costless, novel, environmentally-safe and solar-driven technology. Utilization of non-edible plants in phytoremediation is an ingenious technique as they are used to generate new bioenergy resources along with the remediation of contaminated soils. Some nonfood bioenergy crops such as Salix species, Miscanthus species, Populus species, Eucalyptus species,… More >

Orozco Montes F¹, A Vázquez-Hernández², B Fenton-Navarro¹

Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 1-10, 2019, DOI:10.32604/phyton.2019.04525

Abstract An increased interest in the antioxidant effects of medicinal plants has developed in recent years. Identifying antioxidant compounds present in medicinal plants and elucidating the mechanism by which they prevent oxidation have been the focus of the research community. We performed a systematic and exhaustive review aimed at analyzing the available data regarding the beneficial effects of secondary metabolites in plants. The result of this review is presented as a description of free radicals, as well as cellular and physiological oxidative stress, is provided. The origin and source of antioxidant compounds, and the cellular and molecular mechanism by which they… More >

Hyung-Jo Jung, Heekun Ju

The International Conference on Computational & Experimental Engineering and Sciences, Vol.21, No.1, pp. 6-6, 2019, DOI:10.32604/icces.2019.05761

Abstract The floor response spectrum (FRS) of a nuclear power plant (NPP) structure considering nonlinear behavior of shear walls is numerically investigated. The Hysteretic Material from OpenSees program is applied to a lumped-mass model to consider the nonlinear hysteretic behavior of the element representing the shear wall. Parameters that determine the hysteretic behavior are changed to examine the effects of the parameters on the resulting FRS. The pinching effect, damage due to ductility and energy, and degraded unloading stiffness can be considered with changes in parameter values. The hysteretic behavior of the structure with different parameter values was analyzed. Elements in… More >