Montenegro G, R Pizarro, E Mejías, S Rodríguez

Phyton-International Journal of Experimental Botany, Vol.82, pp. 7-14, 2013, DOI:10.32604/phyton.2013.82.007

Abstract Bee pollen has gained increased interest in recent decades due to its therapeutic, antioxidant, antifungal and anticariogenic properties, as well as its potential use as a functional food product in the food industry because of its nutritional properties. Therefore, it is necessary to identify the chemical components of pollen extracts with different botanical origin, supporting the hypothesis that bee products inherit the characteristics and bioactive properties from the plants they come from. The purpose of this study was to conduct a chemical characterization of samples of pollen collected by Apis mellifera L. from plants native to central Chile, and also… More >

Gil SP, L Seisdedos, ME Reyna, MM Cerana

Phyton-International Journal of Experimental Botany, Vol.81, pp. 205-210, 2012, DOI:10.32604/phyton.2012.81.205

Abstract The goals of this study were (1) to make a careful study of the foliar epidermis of Grindelia cabrerae var. cabrerae, Solidago chilensis y Wedelia buphthalmiflora, native Asteraceae from Argentina which have ornamental potential, and (2) to determine variables that indicate adaptation to xeromorphic environments. Five adult leaves from five plants were selected from each population. All the material was fixed in FAA. The epidermis of both foliar sides were macerated in hypochlorite of sodium, and obtained by scraping. They were stained with safranine and mounted in water-glycerine (1:1). From each slide, five fields were chosen and frequency of the… More >

Velázquez-Sepúlveda I, MC Orozco-Mosqueda, CM Prieto-Barajas, G Santoyo

Phyton-International Journal of Experimental Botany, Vol.81, pp. 81-87, 2012, DOI:10.32604/phyton.2012.81.081

Abstract Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they corresponded to… More >

Pincheira-Ulbrich J

Phyton-International Journal of Experimental Botany, Vol.80, pp. 9-18, 2011, DOI:10.32604/phyton.2011.80.009

Abstract Knowledge about the diversity patterns of vascular epiphytes and vines in the Valdivian rainforest of South America between 2000 and 2010 was systematized. The method was based on the review of publications available in four electronic databases using keywords. The results showed 12 studies conducted in Chile and two in Argentina, mostly in primary forests within protected areas. Research was carried out essentially at the habitat level, although there were one study at a landscape level and two reviews at a regional scale. The samples used came from ground-based observations and only two studies accessed the canopy of emergent trees.… More >

O’Connor-Sánchez¹ A, AV Domínguez-May¹, MA Keb-Llanes¹, YJ Peña-Ramírez², VA Herrera-Valencia¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 25-29, 2010, DOI:10.32604/phyton.2010.79.025

Abstract A reliable protocol for plant regeneration of Mullein Nightshade (Solanum donianum Walp.) was developed from in vitro leaf explants. They were cultured on Murashige and Skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. The formulation that originated most shoots per explant was 8.5 μM zeatin riboside in absence of 1-naphthalene acetic acid. Explants with shoots were transferred to Murashige and Skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. Elongated shoots were individualized, and they rooted readily in half-strength Murashige and Skoog medium without plant growth… More >

Kiehr M¹, R Delhey¹, J Lusto², PJ Paoloni³

Phyton-International Journal of Experimental Botany, Vol.79, pp. 199-202, 2010, DOI:10.32604/phyton.2010.79.199

Abstract A report is given on host plants, distribution, damage and importance of Sclerotinia minor in the southern Pampas and northern Patagonia regions (Argentina). Spontaneous infections were recorded on seven plant species. Sonchus oleraceus and Eruca vesicaria are new hosts for S. minor; Cichorium intybus and Zinnia elegans are new hosts for Argentina. This fungus causes important damage on Helianthus annuus and Lactuca sativa, and it occasionally affects C. intybus and Solanum lycopersicum. Comparative in vitro and in planta studies were carried out with S. minor and S. sclerotiorum isolates obtained from sunflower. More >

Delhey R¹, M Kiehr¹, MI Allievi¹, J Lusto², S Frayssinet¹, B Sidoti Hartmann³, I Kröger¹, PJ Paoloni⁴, D Zappacosta¹, A Servera³

Phyton-International Journal of Experimental Botany, Vol.78, pp. 111-115, 2009, DOI:10.32604/phyton.2009.78.111

Abstract A report is given on plant hosts, distribution, damage and importance of Sclerotinia sclerotiorum in the southern Pampas region and northern Patagonia (Argentina). Spontaneous infections were recorded on 25 plant species, including oil crops, vegetables, and ornamental and invasive plants. Carduus thoermeri, Xanthium cavanillesii, Marrubium vulgare and Ranunculus repens are new hosts of S. sclerotiorum. The records on Helianthus tuberosus, Diplotaxis tenuifolia and Cucurbita pepo are new to Argentina. Sclerotinia sclerotiorum causes important damage on Helianthus annuus, Glycine max, Apium graveolens, Foeniculum vulgare and Lactuca sativa. S. sclerotiorum is also reported on Daucus carota, Petroselinum crispum, Brassica oleracea, B. rapa… More >

Gonzalez-Franco AC¹, L Robles-Hernandez¹, A Nuñez-Barrios¹, JL Strap², DL Crawford³

Phyton-International Journal of Experimental Botany, Vol.78, pp. 83-90, 2009, DOI:10.32604/phyton.2009.78.083

Abstract In order to understand the temporal dynamics of actinomycete communities of the rhizosphere of the desert plant Artemisia tridentata (sagebrush), two complementary methods were used. They were: (1) 16S rDNA-based PCR coupled with denaturing gradient gel electrophoresis (DGGE), and (2) an agar plate enumeration methodology in which three different media were used to quantify total bacteria, actinomycetes, and fungi. The objective of this research were: (1) to obtain a comprehensive picture of the structure of actinomycete populations, and (2) their dynamics in the rhizosphere of young and old sagebrush plants during two distinct seasons. PCR-DGGE analysis showed that actinomycete groups… More >

Orroño DI, RS Lavado^*

Phyton-International Journal of Experimental Botany, Vol.78, pp. 75-82, 2009, DOI:10.32604/phyton.2009.78.075

Abstract Ornamental plants have been proposed for growing in heavy metal (HM) contaminated soils, and also for phytoremediation. We evaluated (1) biomass production and (2) HM accumulation in Pelargonium hortorum. Plants were grown for 16 weeks on HM (cadmium, chromium, copper, lead, nickel and zinc) enriched soils. Treatments were i) control, non-enriched soil, ii) medium concentration treatment, and iii) high concentration treatment. Four destructive harvests were carried out, and roots, stems, leaves, and flowers were analyzed each time. Concentrations of HM were determined using ICP. Significant reductions in biomass production were observed in HM-enriched soils compared with the control. Different indexes… More >

Lagoutte¹ S, M Divo de Sesar², F V ilella³

Phyton-International Journal of Experimental Botany, Vol.78, pp. 31-36, 2009, DOI:10.32604/phyton.2009.78.031

Abstract Annual floral plant crops are produced in cells or pots of various sizes. Varying container size alters the volume which plant roots can explore, which in turn affect plant growth. This work assesed the effect of the initial container size on growth of Petunia x híbrida cv Dream Mix, supplemented with cytokinins after potting. Seedlings obtained in trays of 90 or 288 cells, were transplanted to 1l pots. They were then supplemented with 6-bencyladenine (BA). The assay consisted of 4 treatments: two container sizes by two BA conditions (with and without). Cell size conditioned growth above ground and below ground;… More >