VIVIANE DAL-SOUTO FRESCURA1, HAYWOOD DAIL LAUGHINGHOUSE IV2, THAIS SCOTTI DO CANTODOROW1, SOLANGE BOSIO TEDESCO1*
BIOCELL, Vol.36, No.3, pp. 143-145, 2012, DOI:10.32604/biocell.2012.36.143
Abstract Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of… More >
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