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  • Open Access

    ARTICLE

    The Combination of Achnatherum inebrians Extracts and Soil Microorganisms Inhibited Seed Germination and Seedling Growth in Elymus nutans

    Rui Zhang1, Taixiang Chen1, Zhenjiang Chen1, Hao Chen1, Xuekai Wei1, Malik Kamran1, Chunjie Li1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 567-580, 2024, DOI:10.32604/phyton.2024.047485

    Abstract In a greenhouse experiment, the effects of soil microorganisms and extracts of Achnatherum inebrians on the seed germination and seedling growth of Elymus nutans were studied. The results showed that both the extracts from aboveground and belowground parts of A. inebrians significantly inhibited the germination rate, germination potential, germination index, vigor index, seedling height, root length, and fresh weight of E. nutans, but increased malondialdehyde content, catalase, peroxidase and superoxide dismutase activity of E. nutans seedlings (p < 0.05). The allelopathy of aqueous extracts of the aboveground parts of A. inebrians was stronger than that of the precipitates. Aqueous extracts… More >

  • Open Access

    ARTICLE

    Phenolic Profile and Volatiles of in vitro Propagated Lavandula angustifolia Mill. Seedlings

    Mir Abdullatif Yahya1, Fatma Tunali1, Dilek Killi2,*, Atalay Sökmen2

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 427-444, 2024, DOI:10.32604/phyton.2024.046271

    Abstract An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller, a medicinal aromatic plant that is a prominent source of volatile organic compounds (VOCs). Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators (PGRs), and the growth parameters of the nodal segments were examined. Nodal explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine (BAP). The superior hormonal concentration of Murashige and Skoog (MS) media for the proliferation of shoots from callus cultures (39.33%) was 5 mg/L of 2-Isopentenyl adenine (2iP), and the favorable media for the growth of L. angustifoliaMore >

  • Open Access

    ARTICLE

    Effects of Root Cutting on Morphological Characteristics and Endogenous Hormone Levels of Quercus variabilis Seedlings

    Ruoyi Ni#, Hao Dou#, Shuwen Bi, Tiantian Wang, Jin’e Quan*, Xitian Yang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2907-2920, 2023, DOI:10.32604/phyton.2023.030689

    Abstract The objective of this study was to investigate the effects of root cutting stress on the dynamic changes of endogenous hormone content and growth characteristics of Quercus variabilis roots, and to explore the physiological role of endogenous hormones in regulating root-crown interactions in the short term. The morphological characteristics and endogenous hormone contents of normal roots (no root cutting, CK) and cut roots (cut by 1/3 of the length of the main root, RP) were determined by liquid chromatography, which was combined with mass spectrometry at different levels of different developmental stages. The results showed that the root growth indexes… More >

  • Open Access

    ARTICLE

    An Automatic Classification Grading of Spinach Seedlings Water Stress Based on N-MobileNetXt

    Yanlei Xu, Xue Cong, Yuting Zhai, Zhiyuan Gao, Helong Yu*

    Intelligent Automation & Soft Computing, Vol.37, No.3, pp. 3019-3037, 2023, DOI:10.32604/iasc.2023.040330

    Abstract To solve inefficient water stress classification of spinach seedlings under complex background, this study proposed an automatic classification method for the water stress level of spinach seedlings based on the N-MobileNetXt (NCAM+MobileNetXt) network. Firstly, this study reconstructed the Sandglass Block to effectively increase the model accuracy; secondly, this study introduced the group convolution module and a two-dimensional adaptive average pool, which can significantly compress the model parameters and enhance the model robustness separately; finally, this study innovatively proposed the Normalization-based Channel Attention Module (NCAM) to enhance the image features obviously. The experimental results showed that the classification accuracy of N-MobileNetXt… More >

  • Open Access

    ARTICLE

    Quick and Accurate Counting of Rapeseed Seedling with Improved YOLOv5s and Deep-Sort Method

    Chen Su, Jie Hong, Jiang Wang, Yang Yang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2611-2632, 2023, DOI:10.32604/phyton.2023.029457

    Abstract The statistics of the number of rapeseed seedlings are very important for breeders and planters to conduct seed quality testing, field crop management and yield estimation. Calculating the number of seedlings is inefficient and cumbersome in the traditional method. In this study, a method was proposed for efficient detection and calculation of rapeseed seedling number based on improved you only look once version 5 (YOLOv5) to identify objects and deep-sort to perform object tracking for rapeseed seedling video. Coordinated attention (CA) mechanism was added to the trunk of the improved YOLOv5s, which made the model more effective in identifying shaded,… More >

  • Open Access

    ARTICLE

    Seed Priming with MgCl2, CaCl2, and ZnCl2 as a Biofortification Based-Approach Induces Changes in Anise Seedlings Emergence

    Sibel Day*, Nilüfer Koçak-Şahin

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2461-2471, 2023, DOI:10.32604/phyton.2023.029920

    Abstract Aromatic and medicinal plant species having small seeds have field emergence problems due to low nutrient supply. Therefore, Pimpinella anisum seeds were hydro and osmoprimed with 100 mM MgCl2, CaCl2, and ZnCl2, for 2, 4, and 8 h each to compare their growth attributes during germination and seedling establishment stages. Nontreated seeds were used as control. Both hydro and osmo primed seeds were dried for 48 h before, they were sown in plastic trays in growth room conditions to see the impact of treatments on seedling emergence and growth. The maximum root length (12.90 cm), fresh weight (256.30 mg plant−1),… More >

  • Open Access

    ARTICLE

    Effects of Manganese on the Antioxidant System and Related Gene Expression Levels in the “Hong Yang” Kiwifruit Seedlings

    Chongpei Zheng1, Liangliang Li2, Zhencheng Han2, Weijie Li2,*, Xiaopeng Wen1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2399-2412, 2023, DOI:10.32604/phyton.2023.029891

    Abstract

    To explore how manganese affects the antioxidant system and the expression levels of related genes of “Hong yang” seedlings, the leaves of its tissue cultured seedlings were taken as test materials, and single factor treatment was performed by changing the manganese chloride (MnCl2.4H2O) solution concentration when spraying the leaves. The expression levels of Mn-SOD, POD64 and POD27 genes in leaves were quantitatively analyzed by real-time quantitative PCR (qRT-PCR) at different determination times. Meanwhile, the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2), the activities of antioxidant enzymes, including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). The results showed… More >

  • Open Access

    ARTICLE

    Impact of ZnSO and ZnO Nanoparticles on Seed Germination and Seedling Growth of Lettuce

    Adalberto Benavides-Mendoza1, Rebeca Betancourt-Galindo2, Nazario Francisco-Francisco3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1831-1840, 2023, DOI:10.32604/phyton.2023.028085

    Abstract

    Micronutrient nanoparticles (NPs) are currently an option for chemical fertilization and biostimulation in crops. However, there is little information on the phytotoxic or biostimulatory effects of NPs at low concentrations of some elements, such as Zn. In this study, the effect of low concentrations of Zn oxide (ZnO) NPs on germination, growth variables, and nutritional attributes of lettuce (Lactuca sativa L.) was evaluated in comparison to Zn sulfate. Romaine lettuce seeds were treated with ZnSO4-- × 7H2O and ZnO NPs at Zn molar concentrations of 1 × 10−3, 5 × 10−3, 1 × 10−4, 5 × 10−4, 1… More >

  • Open Access

    ARTICLE

    Biomechanical Response of the Root System in Tomato Seedlings under Wind Disturbance

    Zhengguang Liu1, Jun Yang1, Tobi Fadiji2, Zhiguo Li1,*, Jiheng Ni3

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1071-1090, 2023, DOI:10.32604/phyton.2023.026408

    Abstract Wind disturbance as a green method can effectively prevent the overgrowth of tomato seedlings, and its mechanism may be related to root system mechanics. This study characterized the biophysical mechanical properties of taproot and lateral roots of tomato seedlings at five seedling ages and seedling substrates with three different moisture content. The corresponding root system-substrate finite element (FE) model was then developed and validated. The study showed that seedling age significantly affected the biomechanical properties of the taproot and lateral roots of the seedlings and that moisture content significantly affected the biomechanical properties of the seedling substrate (p < 0.05).… More >

  • Open Access

    ARTICLE

    Nitric oxide alleviates cadmium-impeded growth by limiting ROS accumulation in pea seedlings

    EKHLAQUE A. KHAN1,2,*, HAMDINO M. I. AHMED3, MEENA MISRA1, PALLAVI SHARMA1,4, AMARENDRA N. MISRA1,5, MIRZA HASANUZZAMAN6,*

    BIOCELL, Vol.46, No.12, pp. 2583-2593, 2022, DOI:10.32604/biocell.2022.021732

    Abstract

    Cadmium (Cd) causes oxidative stress, which leads to the oxidation of various biomolecules by the production of reactive oxygen species (ROS) to facilitate programmed cell death (PCD). The antioxidant defense system fails to detoxify ROS when it is produced in excess. Nitric oxide (NO), a gaseous free radical and a phytohormone, regulates various physiological processes of plants. Therefore, this work was undertaken to study the effects of the application of exogenous sodium nitroprusside (SNP, a NO donor) on growth parameters, oxidative stress, accumulation of secondary metabolites, and activities of antioxidant enzymes under Cd stress. Mild (50 µM) and severe (200… More >

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