PAOLA TALIA¹ , EDUARDO J. GREIZERSTEIN¹ , H. ESTEBAN HOPP^1,2 , NORMA PANIEGO¹ , LIDIA POGGIO2 AND RUTH A. HEINZ^1,2*

BIOCELL, Vol.35, No.1, pp. 19-28, 2011, DOI:10.32604/biocell.2011.35.019

Abstract Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16… More >

Hernández-Camacho S¹, E Pérez-Molphe-Balch¹, AG Alpuche-Solís², JF Morales-Domínguez¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 151-162, 2017, DOI:10.32604/phyton.2017.86.151

Abstract Dehydrins or Group 2 Late Embryogenesis Abundant (LEA) proteins play an important role in the response and adaptation to different types of abiotic stresses such as droughts, high salinity and low temperatures. Using PCR techniques, we identified three gene fragments that encoded dehydrin-like proteins in three cactispecies Opuntia ficus-indica (OpfiDHN-like), Leuchtenbergia principis (LepDHN-like) and Mammillaria bombycina (MabDHN-like). Bioinformatic sequence analysis showed an identity between 96 and 97% with the Opuntia streptacantha dehydrin 1 (OpsDHN1) gene, demonstrating that the amplified fragments corresponded to dehydrin-like gene sequences, and that the designed oligonucleotides were effective for similar gene amplification in different cacti genera.… More >

Jin G^1,2, XM Tang³, Y Niu⁴, XY Huang², T Chen², Q Huang², J Zhang², RY Zhou¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 137-142, 2017, DOI:10.32604/phyton.2017.86.137

Abstract In plants, the mitochondrial NADH dehydrogenase (complex I) is a large protein complex transferring electrons to ubiquinone. For the nine chondriogenes encoding complex I subunits (nad1, nad2, nad3, nad4, nad4L, nad5, nad6, nad7, and nad9), an efficient strategy for the cloning of complete coding sequences (CDSs) is important. Specific orthologous portions of untranslated regions (UTRs) were found based on multiple sequence alignments of chondriogene orthologues encoding complex I subunits in plant species. Based on the conservation of partial UTRs, a one-step PCR strategy was conceived for the cloning of CDSs of the nine chondriogene orthologues. Using this strategy, the five… More >

Maria João M. Vasconcelos¹, João Manuel R. S. Tavares¹

The International Conference on Computational & Experimental Engineering and Sciences, Vol.10, No.1, pp. 3-4, 2009, DOI:10.3970/icces.2009.010.003

Abstract In the last years, researchers from the Computational Vision working field have been developing new methods to perform image segmentation for human motion analysis. The development of computational techniques suitable to automatically identify the structures involved is necessary to obtain more representative and robust features to be further used in the analysis of human motion from image sequences.
The first step of human motion analysis from image sequences is strongly related with image segmentation. In fact, the first goal of any system designed for this aim is the identification of the structures’ features to be analysed in the image frames.… More >

Khouloud Cheour^1,*, Mustapha Assarar¹, Daniel Scida¹, Rezak Ayad¹, Xiaolu Gong²

Journal of Renewable Materials, Vol.7, No.9, pp. 877-889, 2019, DOI:10.32604/jrm.2019.06826

Abstract The aim of this study is to show the interest of the mechanical and dynamical properties of glass-flax hybrid composites. Therefore, various staking sequences of glass-flax hybrid composites were manufactured and tested in free vibrations. The damping coefficients were identified by fitting the experimental responses of free-free bending vibrations. The obtained results show that the staking sequences and the position of flax fiber layers in the hybrid composites changed the properties, so a classification of different stacking sequences was established. In fact, the hybrid laminate made of two glass external layers placed on both sides of four flax layers is… More >

Raquel R. Pinho¹, João Manuel R. S. Tavares¹

CMES-Computer Modeling in Engineering & Sciences, Vol.46, No.1, pp. 51-76, 2009, DOI:10.3970/cmes.2009.046.051

Abstract This work addresses the problem of tracking feature points along image sequences. In order to analyze the undergoing movement, an approach based on the Kalman filtering technique has been used, which basically carries out the estimation and correction of the features' movement in every image frame. So as to integrate the measurements obtained from each image into the Kalman filter, a data optimization process has been adopted to achieve the best global correspondence set. The proposed criterion minimizes the cost of global matching, which is based on the Mahalanobis distance. A management model is employed to manage the features being… More >

CMC-Computers, Materials & Continua, Vol.47, No.1, pp. 45-63, 2015, DOI:10.3970/cmc.2015.047.045

Abstract A new theory of strain hardening is developed. Important in its own right, the strain hardening solution is also of decisive use in rigorously defining the historically broad concepts of yield stress and failure stress. Under ideal conditions yield stress is found to represent a 3rd order transition. Failure stress is an explicit "failure of function" criterion rather than just being the primitive notion of breaking into pieces. Computational extensions and opportunities are discussed. More >