S.L. MARUÑAK*, L. LEIVA**, M.E. GARCIA DENEGRI*, P. TEIBLER*, O. ACOSTA DE PÉREZ*
BIOCELL, Vol.31, No.3, pp. 355-364, 2007, DOI:10.32604/biocell.2007.31.355
Abstract A phospholipase A2
has been isolated from Bothrops jararacussu venom from snakes that
inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities
of phospholipase A2
from B. jararacussu as well as isolation details of its. Venom was obtained by milking of
adult snakes which were housing in wood reptile cages of varying dimensions in heated (20–30ºC) rooms.
Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The
enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography
on… More >
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