Xuan Sun^1,2, Xue Wang¹, Bijaya Sharma Subedi³, Yin Jiang^1,2, Di Wang^1,2, Rongxin Gou^1,2, Guojun Zhang², Wenting Xu^4,*, Zunzheng Wei^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2413-2428, 2023, DOI:10.32604/phyton.2023.029667

Abstract The calla lily (Zantedeschia spreng.) is a bulbous flower native to the tropical regions of Africa. Calla lily has gained significant popularity in the international market owing to its intricate morphology and prolonged flowering duration. Despite such advantages, for two sub-groups of calla lily, known as group Zantedeschia and group Aestivae, there are challenges in terms of hybrid production due to the ‘plastome-genome incompatibility’ therebetween. Tissue culture is a fundamental biotechnological tool used in gene editing research, with a focus on disease resistance and flower color in calla lily breeding programs. The present review provides a brief background on the… More >

Yajun Li^1,#, Yumeng Cui^1,#, Bingjie Wang¹, Yue Li¹, Mengmeng Zhang², Silan Dai¹, He Huang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 869-888, 2022, DOI:10.32604/phyton.2022.018659

Abstract Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum, which relies on an optimal regeneration and transformation system. However, the regeneration system of different chrysanthemum cultivars varies, and the regeneration time of most cultivars is long. To screen cultivars with highly efficient regeneration, leaves and shoot tip thin cell layers (tTCL) from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media (MS) supplemented with 1.0–5.0 mg L⁻¹ 6-benzylaminopurine (6-BA) and 0.1–1.0 mg L⁻¹ α-naphthaleneacetic (NAA). The results showed that the most efficient regeneration media were MS +… More >

Otilio Vázquez-Martínez¹, Héctor Gordon Núñez-Palenius^1,*, Eugenio M. Pérez-Molphe Balch^2,*, Mauricio Valencia-Posadas¹, Luis Pérez-Moreno¹, Graciela M. L. Ruiz-Aguilar¹, M. Gómez-Lim³

Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 83-96, 2022, DOI:10.32604/phyton.2022.017281

Abstract In Mexico, there is a need to produce large quantities of plantlets for the establishment and replanting of blue (cv. azul) agave production areas. Most of these plots are within the origin denomination area (DOT, Spanish acronym) of the distilled product of this plant, known as tequila. The objective of this study was to develop an in vitro-propagation protocol for Agave tequilana Weber cv. azul using segmented stems in both: solid and liquid media. A disinfection and in vitro technique were developed to obtain shoots, through plantlets collected in commercial plots, which attained 100% surface-disinfection and budding rate. At the… More >

NÉSTOR CURVETTO1^1,2, PABLO MARINANGELI^1,2, GABRIELA MOCKEL²

BIOCELL, Vol.30, No.3, pp. 497-500, 2006, DOI:10.32604/biocell.2006.30.497

Abstract The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H₂O₂ ) into the nutrient media as chemical sterilizer. A series of H₂O₂ concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits… More >

Sadia^1,3 B, PC Josekutty², SD Potlakayala², P Patel², S Goldman³, SV Rudrabhatla²

Phyton-International Journal of Experimental Botany, Vol.79, pp. 177-181, 2010, DOI:10.32604/phyton.2010.79.177

Abstract A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype… More >

C. Luna, P. Sansberro*, L. Mroginski, J. Tarragó

BIOCELL, Vol.27, No.2, pp. 205-212, 2003, DOI:10.32604/biocell.2003.27.205

Abstract Micropropagation of Ilex dumosa var. dumosa R. (“yerba señorita”) from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in ¹/₄ strength Murashige and Skoog medium (¹/₄ MS) plus 30 gr·L^-1 sucrose and supplemented with 4.4 µM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr·L^-1 sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in ¹/₄ MS (30 gr·L^-1 sucrose, 0.25 % Phytagel^®) with 7.3 µM IBA and 2) 21 days in… More >

Concepción Marino¹, María T. Ponce^1,*, María E. Videla², Sonia Fioretti³, Miguel Cirrincione¹

BIOCELL, Vol.27, No.1, pp. 57-60, 2003, DOI:10.32604/biocell.2003.27.057

Abstract Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L^-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully . More >

M. Y. Ovando-Domínguez¹, M. C. Luján-Hidalgo¹, D. González-Mendoza², A. A. Vargas-Díaz³, N. Ruiz-Lau^1,4, F. A. Gutiérrez-Miceli¹, C. A. Lecona-Guzmán^1,*

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 139-147, 2019, DOI:10.32604/phyton.2019.06546

Abstract Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L^-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L^-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content… More >