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  • Open Access

    ARTICLE

    The uptake of cadmium by Allium cepa var. agrogarum L. and its effects on chromosome and nucleolar behavior in root tip cells

    Wang QL, DH Liu, JY Yue

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 155-161, 2016, DOI:10.32604/phyton.2016.85.155

    Abstract Allium cepa var. agrogarum L. seedlings are sensitive to Cd stress. We used fluorescence imaging to indicate that Cd2+ was localized in cytoplasm in the epidermis of the basal parts of root and vascular tissues after Cd treatment. The nucleoli and the cell walls were the first storage sites of Cd2+. When Cd exposure was prolonged, severe irregularly-shaped nuclei were induced. We used silver nitrate staining to analyze the effects of different concentrations (1–300 μM) of cadmium chloride on chromosome, nucleolus and nucleolus organizer regions (NORs) in root tip cells. Cd2+ induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. More… More >

  • Open Access

    ARTICLE

    Changes in soil organic carbon in the upper Heihe river basin, China

    Qin YY1,2, F Qi1, NM Holden3, JJ Cao4

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 149-154, 2016, DOI:10.32604/phyton.2016.85.149

    Abstract The Heihe River Basin is a globally significant carbon pool, but its soil organic carbon dynamics is poorly understood. Soil samples taken between 2500 m and 4100 m revealed that the majority (>75%) of soil organic carbon was from 0-40 cm. It showed a negative relationship with pH and soil bulk density, and a positive relationship with altitude and soil water content, respectively. From 2005 to 2011, soil carbon content in the upper catchment decreased from 93 g/kg to 53 g/kg. These results suggest that policies should aim to reduce carbon loss by transferring it from the top- to the… More >

  • Open Access

    ARTICLE

    Nutrient characteristics of throughfall and stemflow in the natural forest of Pinus densata in the Tibetan plateau

    Lu J1,2, SX Zhang1*, JP Fang2, WL Zheng2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 142-148, 2016, DOI:10.32604/phyton.2016.85.142

    Abstract Precipitation, throughfall and stemflow were monitored and collected through the rainy season on a natural forest ecosystem of Pinus densata in the Gongbu Nature Reserve in Tibet. The concentrations of N, P, K, Na, Ca, Mg, Si, Mn, Fe, S, Zn and Cu were analyzed in the precipitation, throughfall and stemflow waters, Thereafter, and according to the amount of water of precipitation, throughfall and stemflow, the net input was calculated for each nutrient in the forest. The results showed that: (1) During the entire rainy season, the average concentration of different nutrients in the precipitation of the Pinus densata forest… More >

  • Open Access

    ARTICLE

    Geographical differentiation of wild pepper (Capsicum annuum L. var. glabriusculum) populations from northwestern Mexico

    López-España RG1, S Hernández-Verdugo1, S Parra-Terraza1, F Porras1, Antonio Pacheco-Olvera1, A Valdez-Ortiz2, T Osuna-Enciso3, MD Muy-Rangel3

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 131-141, 2016, DOI:10.32604/phyton.2016.85.131

    Abstract Wild pepper (Capsicum annuum L. var. glabriusculum) is a valuable genetic resource for food and agriculture. The patterns of variation of 12 morphological traits of 17 populations of wild pepper collected across a latitudinal and climate gradients in northwestern Mexico were analyzed in a uniform greenhouse experiment. The morphological traits data were axamined by univariate and multivarite analysis. The relationship between phenotypic variation and latitude of population origen was tested using linear regression analysis. We found high variation within and among populations for most of the measured traits. Populations were clearly differentiated by univariate analysis of variance and principal component… More >

  • Open Access

    ARTICLE

    Total aboveground plant biomass is more strongly affected by climate than species diversity on a grassland in Liaoning, China

    Zhou C1,2, C Busso3, J Liu1, YG Yang1, Y Sun1, YZ Fang1, QQ Zhang1, YB Zhou1,4, YN Wang1,4, Z Zhang4,5, ZW Wang6, YF Yang2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 125-130, 2016, DOI:10.32604/phyton.2016.85.125

    Abstract The objective of this study was to analyze the relationships between total aboveground plant biomass versus altitude, latitude, longitude, mean annual temperature, mean annual precipitation, and species diversity. Simple linear regression analysis was used to study these relationships. Results showed that altitude was significantly, positively (R2= 0.038, P<0.01) related with total plant aboveground biomass. Meanwhile, when longitude (R2= 0.124, P<0.001) and latitude (R2= 0.221, P<0.001) increased, total aboveground biomass decreased. The relationship between biomass and mean annual precipitation was significantly, positively linear (R2= 0.149, P<0.001). Mean annual temperature was significantly, negatively correlated with biomass (R2= 0.145, P<0.001). The relationship between… More >

  • Open Access

    ARTICLE

    Dynamic growth of squash (Cucurbita pepo L.) on a substrate based on vermicompost in the greenhouse

    Orozco V JA1, E Galindo P2, MA Segura C1, M Fortis H1, P Preciado R1, P Yescas C1, JA Montemayor T1

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 117-124, 2016, DOI:10.32604/phyton.2016.85.117

    Abstract The aim of this study was to establish the dynamics of growth and allocation of photosynthates in squash (Cucurbita pepo L.) cultivated in a semiarid region under greenhouse conditions and in an organic substrate. Squash seeds were sown in two substrates (one mineral and other organic). The first consisted of a mixture of sand and pumice on a volume basis, while the organic consisted of sand and pumice mixed with vermicompost, both in plastic pots. The substrates were for two treatments [Inorganic (IT) and Organic Treatment (OT)] with sixteen repetitions on a completely randomized design. After emergence, plants were fertilized… More >

  • Open Access

    ARTICLE

    Glyphosate retention in grassland riparian areas is reduced by the invasion of exotic trees

    Giaccio GCM1, P Laterra2, VC Aparicio3, JL Costa3

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 108-116, 2016, DOI:10.32604/phyton.2016.85.108

    Abstract In this study, we examined some aspects regarding the effect of willow trees (Salix fragilis L.) invasion of grassland riparian environments in the Argentinean Pampas on the runoff reduction, sedimentation and glyphosate retention in the riparian vegetation strip (RVS). To assess the influence of willows on the filtering mechanisms, we performed runoff simulation experiments in plots of 1.5 x 2.5 m, in coastal environments characterized by the presence of willows or the lack of trees. Despite the short length of the experimental plots, the retention of glyphosate in the controls, with no trees, was higher and reached almost 74%. Nevertheless,… More >

  • Open Access

    ARTICLE

    Nitrogen deposition influences the response of Potentilla tanacetifolia to phosphorus addition

    Yang G1,2, Z Zhang1, G Zhang3, H Zhang2, X Han3, CA Busso4

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 100-107, 2016, DOI:10.32604/phyton.2016.85.100

    Abstract Phosphorus is an essential macronutrient for all living plants and plant production. Simultaneously, atmospheric nitrogen deposition also affects plant productivity at a global scale. However, few studies have investigated how plants respond to P addition while simultaneously considering N deposition. We investigated plant biomass, nutrient status and stoichiometric ratios on Potentilla tanacetifolia in response to P fertilization under contrasting N addition rates in a typical meadow steppe in Inner Mongolia, China. Aboveground biomass of P. tanacetifolia increased under increasing levels of P fertilization under conditions of N addition. However, there was no significant change in biomass when only phosphorus was… More >

  • Open Access

    ARTICLE

    Aboveground biomass and concentration of nutrients in semiarid rangeland plant species: Influence of grazing and soil moisture

    Gul B1, M Islam2, S Ahmad3, S Gul1

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 94-99, 2016, DOI:10.32604/phyton.2016.85.094

    Abstract Spatial and temporal patterns of aboveground biomass and nutritive value of rangeland species with respect to the influence of grazing and soil moisture were investigated. The research was conducted during two years at the Tomagh Research Station, near Sanjawi, Ziarat District, Balochistan, Pakistan. This area is protected from grazing since 1998; however, some of the area is open for grazing. Three sites were selected for research purposes: a protected plain, a protected hilly, and an unprotected plain grazed area. Sampling was carried out during the spring, summer and autumn seasons. Results revealed that soil moisture was greater at the protected… More >

  • Open Access

    ARTICLE

    In vitro plant regeneration via indirect organogenesis from different explants of Lathyrus sativus L. and Lathyrus cicera L.

    Li RS1, YJ Tao2, FJ Liu2, X Hu2, QL Xu2, KY Li2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 87-93, 2016, DOI:10.32604/phyton.2016.85.087

    Abstract The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants… More >

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