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  • Open Access

    ARTICLE

    Identification and Evaluation of Insect and Disease Resistance in Transgenic Cry1Ab13-1 and NPR1 Maize

    Yongjing Xi, Zhou Yang, Yukun Jin, Jing Qu, Shuyan Guan, Siyan Liu, Piwu Wang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1257-1274, 2023, DOI:10.32604/phyton.2023.025918

    Abstract PCR detection, quantitative real-time PCR (q-RTPCR), outdoor insect resistance, and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations (T2, T3, and T4) in transgenic maize germplasms (S3002 and 349) containing the bivalent genes (insect resistance gene Cry1Ab13-1 and disease resistance gene NPR1) and their corresponding wild type. Results indicated that the target genes Cry1Ab13-1 and NPR1 were successfully transferred into both germplasms through tested generations; q-PCR confirmed the expression of Cry1Ab13-1 and NPR1 genes in roots, stems, and leaves of tested maize plants. In addition, S3002 and 349 bivalent gene-transformed lines… More >

  • Open Access

    ARTICLE

    Application of Zinc, Iron and Boron Enhances Productivity and Grain Biofortification of Mungbean

    Muhammad Zafar1, Siraj Ahmed1, Muhammad Kashif Munir1, Nawal Zafar1, Muhammad Saqib1, Muhammad Aleem Sarwar2, Saba Iqbal1, Baber Ali3, Naveed Akhtar1, Basharat Ali4,*, Sadam Hussain5,*, Muhammad Saeed6, Mohammad Khalid Al-Sadoon7, Aneela Gulnaz8

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 983-999, 2023, DOI:10.32604/phyton.2023.025813

    Abstract Deficiencies of essential vitamins, iron (Fe), and zinc (Zn) affect over one-half of the world’s population. A significant progress has been made to control micronutrient deficiencies through supplementation, but new approaches are needed, especially to reach the rural poor. Agronomic biofortification of pulses with Zn, Fe, and boron (B) offers a pragmatic solution to combat hidden hunger instead of food fortification and supplementation. Moreover, it also has positive effects on crop production as well. Therefore, we conducted three separate field experiments for two consecutive years to evaluate the impact of soil and foliar application of the aforementioned nutrients on the… More >

  • Open Access

    ARTICLE

    Improving Quantitative and Qualitative Characteristics of Wheat (Triticum aestivum L.) through Nitrogen Application under Semiarid Conditions

    Muhammad Rafiq1, Muhammad Saqib1,*, Husnain Jawad1, Talha Javed2, Sadam Hussain3,*, Muhammad Arif1, Baber Ali4, Muhammad Sultan Ali Bazmi5, Ghulam Abbas1, Marjan Aziz6, Mohammad Khalid Al-Sadoon7, Aneela Gulnaz8, Sobhi F. Lamlom9, Muhammad Azeem Sabir10, Jameel Akhtar6

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1001-1017, 2023, DOI:10.32604/phyton.2023.025781

    Abstract

    Nitrogen (N), the building block of plant proteins and enzymes, is an essential macronutrient for plant functions. A field experiment was conducted to investigate the impact of different N application rates (28, 57, 85, 114, 142, 171, and 200 kg ha−1) on the performance of spring wheat (cv. Ujala-2016) during the 2017–2018 and 2018–2019 growing seasons. A control without N application was kept for comparison. Two years mean data showed optimum seed yield (5,461.3 kg ha−1) for N-application at 142 kg ha−1 whereas application of lower and higher rates of N did not result in significant and economically higher seed… More >

  • Open Access

    ARTICLE

    Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

    Henry López-López1, Nancy Ruiz-Lau2, Rocío Meza-Gordillo1, Víctor Manuel Ruiz-Valdiviezo1, Joseph Galthier Robledo-Luchetti1, Carlos Alberto Lecona-Guzmán1, Juan José Villalobos-Maldonado1, Luc Dendooven3, Joaquín Adolfo Montes-Molina1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1235-1255, 2023, DOI:10.32604/phyton.2023.025716

    Abstract The objective of this work was to evaluate the effect of Beauveria bassiana (Bb 1205) on controlling Fusarium oxysporum f. sp. lycopersici (Fol 17108) in tomato plants in greenhouse conditions. Inoculation of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymes β-1,3-glucanases and chitinases. Inoculation of Bb 1205 occurred at a concentration of 1 × 108 conidia·mL−1, which was administered onto the leaves, directly into the soil and via injection. Infection with Fol 17108 occurred with 1 × 106 spores·mL−1, which were added directly to the soil. Spectrophotometry was used for… More >

  • Open Access

    ARTICLE

    Effect of Rural Sewage Irrigation Regime on Water-Nitrogen Utilization and Crop Growth of Paddy Rice in Southern China

    Menghua Xiao1,*, Yuanyuan Li1,2, Shizong Zheng1

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1215-1233, 2023, DOI:10.32604/phyton.2023.025625

    Abstract

    Reclaimed water irrigation has become an effective mean to alleviate the contradiction between water availability and its consumption worldwide. In this study, three types of irrigation water sources (rural sewage’s primary treated water R1 and secondary treated water R2, and river water R3) meeting the requirements of water quality for farmland irrigation were selected, and three types of irrigation water levels (low water level W1 of 0–80 mm, medium water level W2 of 0–100 mm, and high water level W3 of 0–150 mm) were adopted to carry out research on the influence mechanismS of different irrigation water sources and water… More >

  • Open Access

    ARTICLE

    The Improvement of Soybean Salt Tolerance by Overexpressed GmPAO1

    Yeyao Du, Yang Song, Ye Zhang, Sujie Fan, Hanzhu Zhang, Piwu Wang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1109-1124, 2023, DOI:10.32604/phyton.2023.025503

    Abstract Polyamines play an important regulatory role during plant growth and development and adversity stress, and polyamine oxidase (PAO) is involved in polyamine catabolism. In this study, an up-regulated polyamine oxidase gene GmPAO1 was obtained by transcriptome sequencing analysis and screening at soybean seedling stages. Also, its expression pattern and function were analyzed. The identification results of transgenic GmPAO1 soybean positive lines showed that the relative expression level of GmPAO1 in the overexpressed lines was increased under salt stress. With increasing stress concentration, the seed germination rate decreased. However, the seed germination rate of the overexpressed lines was significantly higher than… More >

  • Open Access

    ARTICLE

    Research on Plant Species Identification Based on Improved Convolutional Neural Network

    Chuangchuang Yuan, Tonghai Liu*, Shuang Song, Fangyu Gao, Rui Zhang

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1037-1058, 2023, DOI:10.32604/phyton.2023.025343

    Abstract Plant species recognition is an important research area in image recognition in recent years. However, the existing plant species recognition methods have low recognition accuracy and do not meet professional requirements in terms of recognition accuracy. Therefore, ShuffleNetV2 was improved by combining the current hot concern mechanism, convolution kernel size adjustment, convolution tailoring, and CSP technology to improve the accuracy and reduce the amount of computation in this study. Six convolutional neural network models with sufficient trainable parameters were designed for differentiation learning. The SGD algorithm is used to optimize the training process to avoid overfitting or falling into the… More >

  • Open Access

    ARTICLE

    Improvement of Selected Morphological, Physiological, and Biochemical Parameters of Banana (Musa acuminata L.) Using Potassium Silicate under Drought Stress Condition Grown in vitro

    Hosny Abdel Aziz1, Mohamed Sharaf2,3, Magdy Omar4, Ahmed Abou El-Yazied5, Nada Ibrahim AlJwaizea6, Shaimaa Ismail5, Mohamed M. A. Omar7, Khadiga Alharbi6,*, Amr Elkelish8,9, Moataz Tawfik10

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1019-1036, 2023, DOI:10.32604/phyton.2023.026769

    Abstract Drought stress has become more common in recent years as a result of climate change impacts on the production of banana crops and other fruit trees. The growth and productivity of Musa spp are severely impacted by the gradual degradation of water resources and the erratic distribution pattern of annual precipitation amount. The aim of the work includes increased drought tolerance in light of water scarcity in the world as a result of the bananas’ being gluttonous for water needs. This investigation was carried out from 2019 to 2020 to study the effect of potassium silicate on morphological growth and… More >

  • Open Access

    ARTICLE

    Toxic and Antifeedant Effects of Different Pesticidal Plant Extracts against Beet Armyworm (Spodoptera exigua)

    Muhammad Asad1, Rashad Rasool Khan2,*, Ahmed B. Aljuboory3, Muhammad Haroon U. Rashid4, Uttam Kumar5, Inzamam Ul Haq6, Aqsa Hafeez7, Ahmed Noureldeen8, Khadiga Alharbi9,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1161-1172, 2023, DOI:10.32604/phyton.2023.026513

    Abstract The beet armyworm (BAW), Spodoptera exigua (Lepidoptera: Noctuidae) is a highly destructive pest of vegetables and field crops. Management of beet armyworm primarily relies on synthetic pesticides, which is threatening the beneficial community and environment. Most importantly, the BAW developed resistance to synthetic pesticides with making it difficult to manage. Therefore, alternative and environment-friendly pest management tactics are urgently required. The use of pesticidal plant extracts provides an effective way for a sustainable pest management program. To evaluate the use of pesticidal plant extracts against BAW, we selected six plant species (Lantana camara, Aloe vera, Azadirachta indica, Cymbopogon citratus, Nicotiana tabacum ,… More >

  • Open Access

    ARTICLE

    The Potential Efficacy of Glycyrrhizic Acid and Its Nanostructure Against Brown Rot of Peach fruits

    Tahsin Shoala1, Basma H. Amin2, Ismail A. S. Rashid3, Fayz A. Abdel-Rahman3, Mohamed E. Khalil3, Khamis Youssef3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1139-1152, 2023, DOI:10.32604/phyton.2023.026515

    Abstract Production of peaches (Prunus persica (L.) Batsch) for both local market and export is increasing each year in Egypt. Brown rot disease, caused by Monilinia laxa and Monilinia fructigena, is considered one of the most important postharvest rots affecting peaches in Egypt and economic losses are increasing. Antifungal activity of glycyrrhizic acid nanoparticles (GA-NPs) and glycyrrhizic acid (GA) at 0.2 and 0.4 mmol/L was investigated as a control for both these brown rot pathogens on peach fruits in both in vitro and in vivo studies. In the in vitro studies, GA-NPs were the most effective as shown by the ability… More >

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