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  • Open Access

    ARTICLE

    Nucleotide Sequence Assessment of Four ORFs of Citrus Tristeza Virus: Evidence of Recombination

    Adel A. Rezk1,2,*, Hala A. Amin2

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.024208

    Abstract Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus (Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism (SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18, p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis and DNA sequencing. SSCP… More >

  • Open Access

    ARTICLE

    The Physiological and Molecular Responses of Exogenous Selenium to Selenium Content and Fruit Quality in Walnut

    Mufang Sun1, Xinran Hui2, Cuiling Tong3, Longyi Yuan2, Dejian Zhang1,2,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.025147

    Abstract To study the effect of exogenous selenium on fruit quality in walnut (Juglans regia L.), 8-year-old walnut (Qingxiang) was taken as the research object. In the fruit expansion stage, 300 mg/L of sodium selenate, yeast selenium and sodium selenite solutions were applied on the leaf of walnut, and the selenium levels in leaves, pericarp and kernel were determined at the ripening stage. The fruit quality, mineral nutrient content, antioxidant enzyme activity, and related genes’ expression were analyzed. The results showed that the three exogenous selenium increased the selenium levels in leaves, pericarp and kernel of walnut. They also significantly increased… More >

  • Open Access

    ARTICLE

    Leaf Wettability Difference Among Tea Leaf Ages and Analysis Based on Microscopic Surface Features

    Qingmin Pan1, Yongzong Lu1, Liang Xue2, Yongguang Hu1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.023437

    Abstract The wettability of leaf surface, commonly represented by contact angle (CA), affects various physiological and physical processes. The present study aims to better understand the wettability of tea leaves and elucidate its influence on the energy barrier of the droplet condensation process. The CA values of different leaf ages (young, mature and old) of five famous tea cultivars (Maolu, longjing 43, Huangjinya, Zhongcha 108 and Anji Baicha) were measured via the sessile drop method, and the micro-morphology of two cultivars leaves (Maolu, Zhongcha 108) was investigated by a 3D super depth-of-field digital microscope. Specifically, two radically distinctive types of CA… More >

  • Open Access

    ARTICLE

    Conventional Breeding and Molecular Markers for Blast Disease Resistance in Rice (Oryza sativa L.)

    Adel A. Rezk1,2, Mohamed M. El-Malky3, Hossam S. El-Beltagi1,4,*, Mohammed Al-daej1, Kotb A. Attia5,6

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.024645

    Abstract Monogenic lines, which carried 23 genes for blast resistance were tested and used donors to transfer resistance genes by crossing method. The results under blast nursery revealed that 9 genes from 23 genes were susceptible to highly susceptible under the three locations (Sakha, Gemmeza, and Zarzoura in Egypt); Pia, Pik, Pik-p, Piz-t, Pita, Pi b, Pi, Pi 19 and Pi 20. While, the genes Pii, Pik-s, Pik-h, Pi z, Piz-5, Pi sh, Pi 3, Pi 1, Pi 5, Pi 7, Pi 9, Pi 12, Pikm and Pita-2 were highly resistant at the same locations. Clustering analysis confirmed the results, which… More >

  • Open Access

    ARTICLE

    Effects of Fertilization on Soil CO2 Efflux in Chinese Hickory (Carya cathayensis) Stand

    Juan Liu1,2,*, Meiqun Zheng1, Xueshuang Chen1

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.023397

    Abstract Chinese hickory (Carya cathayensis Sarg.) is a popular nut tree in China, but there is little information about the influences of fertilization on soil CO2 efflux and soil microbial biomass. This study evaluated the short-term effects of different fertilizer applications on soil CO2 efflux and soil microbial biomass in Chinese hickory stands. Four fertilizer treatments were established: control (CK, no fertilizer), inorganic fertilizer (IF), organic fertilizer (OF), and equal parts organic and inorganic N fertilizers (OIF). A field experiment was conducted to measure soil CO2 effluxes using closed chamber and gas chromatography techniques. Regardless of the fertilization practices, soil CO2More >

  • Open Access

    ARTICLE

    Comparative Transcriptome Analysis of Salt-Stress-Responsive Genes in Rice Roots

    Rui Song1, Yan Huang2, Xin Ji3, Yunfei Wei3, Qiuyuan Liu3, Shumei Li3, Juan Liu3,*, Pengfei Dong1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.023081

    Abstract Soil salinity greatly impairs plant growth and crop productivity. Rice (Oryza sativa L.) is a salt-sensitive crop. To better understand the molecular mechanisms of salt tolerance in roots, the BGISEQ-500 sequencing platform was employed to elucidate transcriptome changes in rice roots after 0, 3, 24, and 72 h of salt stress. The results showed that root K+ content decreased and Na+ content increased rapidly after the initial stage of salt stress, but that fresh and dry weight in root did not significantly reduce. Compared to the control (no salt stress), 1,292, 453, and 486 differentially expressed genes (DEGs) were upregulated,… More >

  • Open Access

    ARTICLE

    Extraction, Antioxidant Activity and Identification of Flavonoids from Root Tubers of Kosteletzkya virginica

    Yu Zai*, Shaohua Liu

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022576

    Abstract Kosteletzkya virginica (K. virginica) is used for revegetation of salt-affected coastal tidal flats and as a raw material of biodiesel. K. virginica root tuber, a biowaste with low economic value, is rich in bioactive compounds. This study aimed to extract and identify flavonoids from K. virginica root tubers. The optimal extraction conditions were 1/25 (w/v) solid/liquid ratio, 40% ethanol concentration at 40°C for 60 min. Under these conditions, 65.2 ± 3.7 mg/g total flavonoid content was extracted from the roots, which were collected from salinized soil in late autumn of the third year. Antioxidant activity was evaluated through 1,1-diphenyl-2-picrylhydrazyl, hydroxyl… More >

  • Open Access

    ARTICLE

    Physiological and Biochemical Characteristics and Response Patterns of Salinity Stress Responsive Genes (SSRGs) in Wild Quinoa (Chenopodium quinoa L.)

    Yurong Jiang1, Muhammad Yasir1, Yuefen Cao1, Lejia Hu1, Tongli Yan1, Shuijin Zhu2,*, Guoquan Lu1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022742

    Abstract Cultivating salt-tolerant crops is a feasible way to effectively utilize saline-alkali land and solve the problem of underutilization of saline soils. Quinoa, a protein-comprehensive cereal in the plant kingdom, is an exceptional crop in terms of salt stress tolerance level. It seems an excellent model for the exploration of salt-tolerance mechanisms and cultivation of salt-tolerant germplasms. In this study, the seeds and seedlings of the quinoa cultivar Shelly were treated with different concentrations of NaCl solution. The physiological, biochemical characteristics and agronomic traits were investigated, and the response patterns of three salt stress-responsive genes (SSRGs) in quinoa were determined by… More >

  • Open Access

    ARTICLE

    Effects of Different Chinese Hickory Husk Returning Modes on Soil Nutrition and Microbial Community in Acid Forest Soil

    Qian Liu1, Sayikal Duyxanale1, Yongqian Tang2, Xinyu Shen1, Yuanlai Zhao1, Xinru Ma1, Shuai Shao1, Chenfei Liang1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.023225

    Abstract Chinese hickory (Carya cathayensis Sarg.) is an important economic forest in Southeastern China. A large amount of hickory husk waste is generated every year but with a low proportion of returning. Meanwhile, intensive management has resulted in soil degradation of Chinese hickory plantations. This study aims to investigate the effects of three Chinese hickory husk returning modes on soil amendment, including soil acidity, soil nutrition, and microbial community. The field experiment carried out four treatments: control (CK), hickory husk mulching (HM), hickory husk biochar (BC), and hickory husk organic fertilizer (OF). The phospholipid fatty acid (PLFA) biomarker method was employed… More >

  • Open Access

    ARTICLE

    Overexpression of Wheat TaELF3-1BL Delays Flowering in Arabidopsis

    Junsheng Sun#, Haozhen Zhang#, Mingxia Zhang, Sishen Li, Yanrong An*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022225

    Abstract EARLY FLOWERING 3 (ELF3), a light zeitnehmer (time-taker) gene, regulates circadian rhythm and photoperiodic flowering in Arabidopsis, rice, and barley. The three orthologs of ELF3 (TaELF3-1AL, TaELF3-1BL, and TaELF3-1DL) have been identified in wheat too, and one gene, TaELF3-1DL, has been associated with heading date. However, the basic characteristics of these three genes and the roles of the other two genes, TaELF3-1BL and, TaELF3-1AL, remain unknown. Therefore, the present study obtained the coding sequences of the three orthologs (TaELF3-1AL, TaELF3-1BL, and TaELF3-1DL) of ELF3 from bread wheat and characterized them and investigated the role of TaELF3-1BL in Arabidopsis. Protein sequence… More >

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