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  • Open Access

    ARTICLE

    An Efficient Plant Regeneration System of Hydrangea bretschneideri Dipp via Stem Segments as Explants

    Si ran Wang#, Jia Yan#, Bu er Ha, Yu’e Bai*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.013693

    Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL−1 6-BA and 0.5 mgL−1 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation coefficient… More >

  • Open Access

    ARTICLE

    Ameliorative Role of Pre-Sowing Proline Treatment in Coriandrum sativum L. Seedlings under Mercury Toxicity

    Dhriti Kapoor1, Kapani Kavani1, Amandeep Rattan2, Marco Landi3,*, Anket Sharma4,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.012359

    Abstract Heavy metal toxicity is one of the major ecosystem concerns globally in present time and is also responsible for significant threat to agronomic crops. The current work was conducted to investigate the possible ameliorative role of proline in Coriandrum sativum L. seedlings treated with mercury (Hg). The seedlings were exposed to different concentrations of Hg (0, 0.1, 0.3 and 0.5 mM) for 20 days. The effects of pre-sowing treatment with proline were studied on C. sativum seedlings in terms of pigment (chlorophylls, carotenoids and anthocyanins), malondialdehyde (MDA), antioxidant compound (glutathione, total phenolic compounds, ascorbic acid) and osmolytes (proline, glycine betaine).… More >

  • Open Access

    ARTICLE

    Interaction of Acaena elongataL. with Arbuscular Mycorrhizal Fungi under Phosphorus Limitation Conditions in a Temperate Forest

    Yasmin Vázquez-Santos1, Yuriana Martínez-Orea1, Javier Álvarez-Sánchez1, Noé Manuel Montaño2, Sara Lucía Camargo-Ricalde2, Silvia Castillo-Argüero1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014208

    Abstract The aim of this study was to analyze the performance of Acaena elongata colonized by arbuscular mycorrhizal fungi (AMF) to different phosphorus (P) concentrations, as a measure of AMF dependency. A. elongata, is a species from soils where P availability is limited, such as temperate forests. Our research questions were: 1) How do different P concentrations affect the AMF association in Acaena elongata, and 2) How does the AMF association influence A. elongata’s growth under different P concentrations? A. elongata’s growth, P content in plant tissue, AMF colonization and dependency were measured under four P concentrations: control (0 g P… More >

  • Open Access

    ARTICLE

    Foliar Application of Phosphorus Enhances Photosynthesis and Biochemical Characteristics of Maize under Drought Stress

    Zahoor Ahmad1,2,*, Ejaz Ahmad Waraich3, Muhammad Zia ur Rehman4, Muhammad Ashar Ayub4, Muhammad Usman4, Hesham Alharby5, Atif Bamagoos5, Celaleddin Barutçular1, Muhammad Ali Raza6, Fatih Çiğ7, Ayman El Sabagh7,8,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.013588

    Abstract Water is essential for the growth period of crops; however, water unavailability badly affects the growth and physiological attributes of crops, which considerably reduced the yield and yield components in crops. Therefore, a pot experiment was conducted to investigate the effect of foliar phosphorus (P) on morphological, gas exchange, biochemical traits, and phosphorus use efficiency (PUE) of maize (Zea mays L.) hybrids grown under normal as well as water deficit situations at the Department of Agronomy, University of Agriculture Faisalabad, Pakistan in 2014. Two different treatments (control and P @ 8 kg ha−1 ) and four hybrids (Hycorn, 31P41, 65625,… More >

  • Open Access

    ARTICLE

    Poultry Manure as an Organic Fertilizer with or without Biochar Amendment: Influence on Growth and Heavy Metal Accumulation in Lettuce and Spinach and Soil Nutrients

    Hira Javaid Siddiqui1, Shamim Gul1,2,*, Attiq-ur-Rehman Kakar3, Umbreen Shaheen4, Gul Bano Rehman1, Naqeebullah Khan3, Samiullah3

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.011413

    Abstract This pot-based study investigated the influence of poultry manure and 1:1 mixture of poultry manure + biochar (produced from farmyard manure [FYM] or wood), on the biomass production and concentration of heavy metals in leaves of lettuce and spinach. The concentration of mineral nitrogen (N) and soluble inorganic phosphorus (P) of soils cultivated with these vegetables was also investigated. The application of poultry manure or FYM biochar in soil as 10% (equivalent to 60 t ha–1 , an estimated 1726.8 kg ha–1 N in poultry manure and 1353.9 kg ha–1 N in FYM) and 15% amendment (equivalent to 90 t… More >

  • Open Access

    ARTICLE

    Fine Mapping and Candidate Gene Prediction of the Quantitative Trait Locus qPL8 for Panicle Length in Rice

    Lin Zhang1,2,*, Yiting Zou1, Zhong Bian1, Dong Xie1, Hadi Yeilaghi1, Xiaolei Fan1,2, Dongsheng Zhao1,2, Qiaoquan Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014880

    Abstract Rice panicle is the sink organ where assimilation product accumulates, and its morphology determines the rice yield. Panicle length has been suggested as a yield-related trait, but the genetic factor for its control is still limited. In this study, we carried out fine-mapping of qPL8, a QTL identified for panicle length in our previous work. Near isogenic line (NIL) with qPL8 exhibited elongated panicle without obvious effect on other panicle elements. With five key recombinants from NIL population, the locus was finally narrowed down to a 278-kb region, where 44 genes are annotated. By comparing the genomic sequence of two… More >

  • Open Access

    ARTICLE

    Phenotype Analysis and Fine Mapping of the Male Sterile Mutant ms10 in Rice (Oryza sativa L.)

    Gang Wei#, Hongxia Yang#, Jingwen Wu, Zixian Xiong, Nan Wang*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014505

    Abstract There is a positive correlation between fertility and yield, and the decrease of fertility is bound to a greatly reduced crop yield. Male sterile mutants can be used in hybrid rice. Therefore, rice male sterility has an important value in research and application, and the study of related mutants is also very vital. The mutant ms10 (male sterile 10) reported in this study was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B. There was no significant difference between the ms10 and wild type in the vegetative growth stage. However, in the reproductive growth stage, ms10More >

  • Open Access

    ARTICLE

    Laboratory-and Field-Phenotyping for Drought Stress Tolerance and Diversity Study in Lentil (Lens culinaris Medik.)

    Shahida Akter, Israt Jahan, Md. Amir Hossain*, Mohammad Anwar Hossain*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014411

    Abstract Drought susceptibility and low genetic variability are the major constraints of lentil (Lens culinaris Medik.) production worldwide. Development of an efficient pre-field drought phenotyping technique and identification of diversified drought tolerant lentil genotype(s) are therefore vital and necessary. Two separate experiments were conducted using thirty diverse lentil genotypes to isolate drought tolerant genotype(s) as well as to assess their diversity. In both of the experiments, significant (p ≤ 0.01) variation in genotype (G), treatment (T) and G X T was observed for most of the studied traits. In experiment I, genotypes were examined for drought tolerance at the seedlings stage… More >

  • Open Access

    ARTICLE

    Alleviation of Cadmium Toxicity to Medicago Truncatula by AMF Involves the Changes of Cd Speciation in Rhizosphere Soil and Subcellular Distribution

    Yuying Jiang1,#, Renhua Huang1,#, Lei Jiang1, Ke Chen1,*, Wenkun Zhu2,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014376

    Abstract Arbuscular mycorrhizal fungi (AMF) can improve plant tolerance to several abiotic stresses, including heavy metals, drought or salinity exposure. However, the role of AMF in alleviation of soil cadmium (Cd)-induced toxicity to plants is still largely unknown. In this study, Cd speciation in soil and subcellular distribution of Cd were used to characterize the roles of application AM fungi in the alleviation of Cd toxicity in alfalfa plants. Our results showed that the addition of Glomus mosseae in Cd contaminated soil (10 mg/Kg) significantly increased soil pH, cation exchange capacity (CEC) and organic matter in rhizosphere soil with Medicago truncatulaMore >

  • Open Access

    ARTICLE

    Anthocyanin Profiles in Grape Berry Skins of Different Species of Wine Grapes in Shanxi, China

    Wei Tan1, Mingxiu Xu1, Siqi Xie1, Yan Zhang1, Shuai Wu1, Qinyan Zou1, Qifeng Zhao2, Qingliang Li3,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014082

    Abstract To understand the anthocyanin characteristics of wine grape varieties, the anthocyanin composition and content of 31 wine grape varieties were analyzed to explore the use of anthocyanins as chemical fingerprints to distinguish varieties. Results showed that a total of 21 anthocyanins were detected in the skins, including cyanidin, delphinidin, petunidin, peonidin and malvidin 3-monoglucosides (or 3,5-diglucosides) along with the corresponding acetyl and p-coumaroyl derivatives. The highest and lowest total amount of anthocyanins were detected in ‘Ruby Cabernet’ and ‘Muscat Rouge’, respectively. In the 21 Vitis vinifera grapes, there were 3~11 monoglucoside anthocyanins detected, however, there were 4 to 9 monoglucoside… More >

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