LAN ZHOU^1,#, QI ZHANG^2,#, BO TIAN^1,*, FENG YANG^1,*

BIOCELL, Vol.48, No.7, pp. 1081-1093, 2024, DOI:10.32604/biocell.2024.049878

Abstract Background: Glioblastoma, a notably malignant tumor within the central nervous system, is distinguished by its aggressive behavior. Silvestrol, a robust inhibitor of the RNA helicase eukaryotic initiation factor 4A (eIF4A), has shown significant potential as an anticancer compound. Yet, the impact of silvestrol on glioblastoma, especially its molecular mechanisms, has not been fully elucidated. Methods: This investigation employed a variety of in vitro assays, such as cell counting kit-8 (CCK-8), clonogenic, 5-ethynyl-2′-deoxyuridine (EDU), wound healing, and flow cytometry, to evaluate cell cycle progression, apoptosis, cell viability, and migration. Western blot analysis was also performed to study… More >

JIAOE SHENG¹, ZUMIN YI², SANSHAN HE¹, QINGCHAO WU¹, XIA HUANG¹, GUOQING YAN¹, YUFANG DAI^1,*, LINCHONG SU^1,*

BIOCELL, Vol.48, No.7, pp. 1095-1104, 2024, DOI:10.32604/biocell.2024.028421

Abstract Introduction: Cholic acid (CA) is a natural steroid useful in treating chronic bronchitis and cholecystitis. On the other hand, its potential impact on osteoarthritis (OA) is unknown. Objective: Using an in vitro and in vivo osteoarthritis model, we sought to assess the chondroprotective properties of CA. Methods: We employed the Cell Counting Kit-8 to measure the impact of CA on chondrocyte activity to assess the toxicity of the cells. Multiple molecular biology experimental techniques were used to investigate potential signaling pathways that CA may use to prevent inflammation and give chondrocytes protection. Furthermore, how CA affects the OA… More >

CARLOS ALBERTO-SILVA^*, BRENDA RUFINO DA SILVA

BIOCELL, Vol.48, No.6, pp. 897-904, 2024, DOI:10.32604/biocell.2024.050443

Abstract Venom snake-derived peptides have multiple biochemical, pharmacological, and toxicological profiles, allowing for the discovery of new medicinal products and therapeutic applications. This review specifically examines the fundamental elements of neuroprotection offered by different oligopeptides derived from snake venom. It also includes a brief evaluation of short peptides that are being considered as potential therapeutic agents. Proline-rich peptides and tryptophyllin family peptides isolated from the crude venom of Viperidae family snakes, specifically Bothrops atrox, Bothrops jararaca, and Bothrops moojeni, have been shown to have pro-survival properties, the ability to reduce oxidative stress, and the ability to promote cell viability More >

LINGLI ZHANG^1,2,#, YAN LI^1,#, JINGXIN MAO^1,*

BIOCELL, Vol.48, No.6, pp. 905-922, 2024, DOI:10.32604/biocell.2024.050396

Abstract Hepatocellular carcinoma (HCC) remains a prevalent and challenging malignancy globally, characterized by its numerous causal factors and generally unfavorable prognosis. In the relentless pursuit of effective treatment modalities, natural products have emerged as a promising and relatively non-toxic alternative, garnering significant interest. The integration of natural products with contemporary medical research has yielded encouraging therapeutic outcomes in the management of HCC. This review offers a comprehensive overview of the causal factors underlying HCC, and the diverse treatment options available, and highlights the advancements made by natural products in anti-HCC research. Particularly, we provide an outline More >

YUANYUAN LIAO^1,2,3, XINSI LI^2,3, QIAN LI^2,3, YIZHONG WANG⁴, XIUJUN TAN^1,2,3, TING GONG^2,3,5,*

BIOCELL, Vol.48, No.6, pp. 873-887, 2024, DOI:10.32604/biocell.2024.050324

Abstract As we are facing an aging society, anti-aging strategies have been pursued to reduce the negative impacts of aging and increase the health span of human beings. Gut microbiota has become a key factor in the anti-aging process. Modulation of gut microbiota by fecal microbiota transplantation (FMT) to prevent frailty and unhealthy aging has been a hot topic of research. This narrative review summarizes the benefits of FMT for health span and lifespan, brains, eyes, productive systems, bones, and others. The mechanisms of FMT in improving healthy aging are discussed. The increased beneficial bacteria and More >

GUIBIN ZHONG^1,2,#, SHURONG WANG^3,#, YUJIN HE⁴, DAMING FENG¹, KE WEI¹, YANQIU YANG¹, JIANWEI CHEN^1,2,*, JUNLING CHEN^1,*

BIOCELL, Vol.48, No.6, pp. 1001-1008, 2024, DOI:10.32604/biocell.2024.050294

Abstract Background: Hypertrophy of the ligamentum flavum (HLF) is a common contributor to spinal stenosis which results in significant neurological impairments. Circular RNA (circRNA) circ_0003609 has been linked to HLF; however, the exact mechanism by which it causes this disease is unclear. Methods: Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference. Cell proliferation and fibrosis-related gene expression were checked by the Cell Counting Kit-8 (CCK-8) assay and western blotting. CircBank’s prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment. The function of the miR-155/sirtuin 1 More >

LITING WEN^#, XIAOXIA ZENG^#, PEIXIONG CHEN, DAPENG ZHAO, YANGYANG LI, XIANHAI ZENG^*

BIOCELL, Vol.48, No.6, pp. 991-999, 2024, DOI:10.32604/biocell.2024.050219

Abstract Background: Cochlear hair cell injury is a common pathological feature of hearing loss. The basic helix-loop-helix family, member e40 (Bhlhe40), a gene belonging to the basic helix-loop-helix (bHLH) family, exhibits strong transcriptional repression activity. Methods: Oxidative damage, in House Ear Institute-Organ of Corti 1 (HEI‑OC1) cells, was caused using hydrogen peroxide (HO). The Ad-Bhlhe40 particles were constructed to overexpress Bhlhe40 in HEI-OC1 cells. Various assays including cell counting kit-8 (CCK-8), terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay (TUNEL), flow cytometry, immunofluorescence, and corresponding commercial kits were employed to investigate the impacts of Bhlhe40 on cell viability, apoptosis,… More > Graphic Abstract

MINGYUE HUANG^1,#, JUNFEI DAI^1,#, XIANLIANG ZHONG², JIN WANG², JIANZHONG XU², BO DU^2,*

BIOCELL, Vol.48, No.6, pp. 889-896, 2024, DOI:10.32604/biocell.2024.050097

Abstract Chronic subdural hematoma (CSDH) is a disease characterized by capsuled blood products that progressively occupy the intracranial space, causing intracranial hypertension and compression in the brain. CSDH frequently occurs in all demographics, especially in the elderly, but the pathogenesis of CSDH remains unclear. In this review, we discuss the origin, development, and current treatment strategies of CSDH. For the first time, we analyzed the cellular and molecular compositions of hematoma membranes with a focus on neomembrane formation, a complex early-stage interactive event in hematoma pathogenesis. We hypothesize that in patients with CSDH, dural border cells… More >

JULING FENG^1,3, HAODONG CHEN¹, YANGBO LIU¹, QIDI AI¹, YANTAO YANG¹, LEI ZHAO⁴, SHIFENG CHU^2,#,*, NAIHONG CHEN^1,2,#,*

BIOCELL, Vol.48, No.6, pp. 981-990, 2024, DOI:10.32604/biocell.2024.050011

Abstract Introduction: Chemokine-like factor 1 (CKLF1) is a chemokine that is overexpressed in several diseases. Our previous findings revealed a significant increase in CKLF1 expression in the ischemic brain, suggesting its potential as a therapeutic target for ischemic stroke. Methods: In this study, we examined the expression dynamics of CKLF1 in both in vivo and in vitro models of ischemic cardiac injury. Myocardial infarction (MI) was induced in vivo by ligation of the left anterior descending artery (LAD) of the rat heart. The levels of CKLF1, Creatine Kinase MB Isoenzyme (CK-MB), and Lactate dehydrogenase (LDH) in the serum were… More > Graphic Abstract

MOHD ATHAR^1,*, ANU MANHAS², NISARG RANA², AHMAD IRFAN³

BIOCELL, Vol.48, No.6, pp. 935-944, 2024, DOI:10.32604/biocell.2024.049891

Abstract Computational methods have significantly transformed biomedical research, offering a comprehensive exploration of disease mechanisms and molecular protein functions. This article reviews a spectrum of computational tools and network analysis databases that play a crucial role in identifying potential interactions and signaling networks contributing to the onset of disease states. The utilization of protein/gene interaction and genetic variation databases, coupled with pathway analysis can facilitate the identification of potential drug targets. By bridging the gap between molecular-level information and disease understanding, this review contributes insights into the impactful utilization of computational methods, paving the way for More >