LEI WANG^1,2, ZHI-HANG WANG¹, NIAN-PING CAO¹, BOBO CHEN¹, CHONG-JUN HUANG¹, LEI YANG¹, YE TIAN^1,*

BIOCELL, Vol.48, No.2, pp. 191-203, 2024, DOI:10.32604/biocell.2023.045349

Abstract Estrogen-related receptor alpha can significantly affect cell metabolism and play key regulatory roles in healthy and diseased organisms. ERRα is also related to the onset and progression of various cancer types. ERRα is primarily expressed in metabolically active tissues and regulates the transcription of metabolic genes in such tissues. It coordinates metabolism and energy demand, affects osteoblasts, osteoclasts, and chondrocytes, promotes muscle regeneration, participates in angiogenesis, and regulates cell aging. In this study, the literature related to the identification of ERRα in skeletal, muscular, and vascular systems was reviewed to further elucidate this receptor. More >

QINGJIAN HE¹, HUIXIN ZHU^2,3, SHANHONG FANG^4,5,*

BIOCELL, Vol.48, No.2, pp. 205-216, 2024, DOI:10.32604/biocell.2023.045109

Abstract Introduction: Dexamethasone (Dex) caused impaired osteoblast differentiation and oxidative stress (OS) in bone marrow mesenchymal stem cells (BMSCs). This work sought to elucidate the precise molecular pathway through which Dex influences osteogenic differentiation (OD) and OS in BMSCs. Methods: The expression of Runt-related transcription factor 1 (RUNX1) and alpha-2 macroglobulin (A2M) was assessed in Dex-treated BMSCs using qRT-PCR and Western Blot. Following the functional rescue experiments, cell proliferation was determined by MTT assay, reactive oxygen species (ROS) expression by DCFH-DA fluorescent probe, lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) expression by kits, OD by alkaline… More >

JINNI MA^#, MEILIN ZHOU^#, XIN XU, XINYAO GAO, HAIXIA WANG, JINHUA SHEN, LU XUE^*

BIOCELL, Vol.48, No.2, pp. 239-252, 2024, DOI:10.32604/biocell.2023.045076

Abstract Background: Placenta specific 8 (PLAC8) is a candidate oncogene involved in the development and progression of solid tumors. However, the status of PLAC8 in lung cancer (LC), especially non-small cell lung cancer (NSCLC) is still not lucid. Methods: Tissue microarray analysis (TMA) was performed to detect the expression patterns of PLAC8 in LC tissues and cell lines. Then a series of cellular experiments were performed fto assess cell proliferation, cell cycle profiles, and cell motility to explore the role of PLAC8 in NSCLC-derived cell lines: H1299 and A549. Results: TMA results showed that PLAC8 played complex and even contradictory roles… More >

YANG XU^1,#, HONGYUN LI^1,#, GE YOU^2,*

BIOCELL, Vol.48, No.2, pp. 229-237, 2024, DOI:10.32604/biocell.2023.045030

Abstract Background: Colorectal cancer (CRC) constitutes the leading cause of death worldwide. Chemoresistance and tumor immune evasion are critical contributors to therapeutic failure in cancer patients. CKLF-like MARVEL transmembrane domain-containing 6 (CMTM6) is aberrantly expressed in various cancers and can regulate tumor immunity. However, its role in chemoresistance and tumor immunity of CRC is not well understood. Methods: Online bioinformatics tools were used to analyze expression and prognosis of CMTM6 in CRC patients. CRC cells were transfected with si-CMTM6. Subsequently, the effects on CRC cell viability and chemoresistance were investigated by CCK-8 assay and flow cytometer. Furthermore, CRC cell-induced macrophage recruitments… More > Graphic Abstract

BINGJIE WANG¹, YEWEN ZHOU^1,2,3, TONGJUAN NIU¹, MENGQING YIN¹, SHENG CUI^1,2,3,*

BIOCELL, Vol.48, No.2, pp. 327-338, 2024, DOI:10.32604/biocell.2023.044630

Abstract Introduction: The regulation of thyroid-stimulating hormone (TSH) synthesis involves neurotransmitters, with melatonin being a subject of ongoing debate. TSH transcription, synthesis, and secretion from the pituitary pars distalis (PD) is primarily regulated in a photoperiodic manner by thyrotropin-releasing hormone (TRH). In contrast, in the pituitary pars tuberalis (PT), mRNA transcription and alpha/beta chain synthesis, but not secretion, of a TSH-like product is regulated by melatonin. Conversely, non-photoperiodic melatonin might also affect the secretion of a TSH-like product from the PT. Nevertheless, the impact of exogenous melatonin on the underlying PD-TSH synthesis remains unclear. Casein kinase 1α (CK1α) plays a negative… More >

WEI ZHENG¹, CHENG LIU^2,*

BIOCELL, Vol.48, No.2, pp. 293-301, 2024, DOI:10.32604/biocell.2023.044211

Abstract Objective: Intracranial aneurysm (IA) represents a devastating disease with high rates of disability and mortality, which is initiated by dysfunction of endothelial cells (ECs). Evidence suggests the dysregulation of the E3 ubiquitin ligase family during EC injury. In this work, the role of an E3 ubiquitin ligase, casitas B lymphoma-B (CBL-B), was explored in human brain microvascular EC (HBMEC) function through the NLRP3 pathway. Methods: In vitro IA model was induced by treating HBMECs with oxidized low-density lipoprotein (ox-LDL). The levels of CBL-B and pyroptosis-related proteins NLRP3, ASC, cleaved caspase-1, and GSDME-N were determined by real-time-quantitative polymerase chain reaction and… More >

ANWAR ALI^1,#, SREEKANTH REDDY OBIREDDY^2,#, WING-FU LAI^3,*

BIOCELL, Vol.48, No.2, pp. 163-171, 2024, DOI:10.32604/biocell.2023.043839

Abstract Farnesol is a C15 isoprenyl alcohol generated from the hydrolysis of farnesyl diphosphate under the action of farnesol synthase in plants. Although this sesquiterpenoid alcohol has been exploited in a wide variety of industrial applications, serious discussions on the possible use of this agent in disease treatment have been scant until now. In this article, we review the current understanding of the physiological properties of farnesol and the potential use of the agent as a candidate for treatment development. In fact, since the turn of the last century, farnesol has been found not only to trigger apoptosis in tumor cells… More >

VAN THI TUONG NGUYEN^1,2, KHUONG DUY PHAM^1,2,3, HUONG THI QUE CAO^1,2, PHUC VAN PHAM^1,2,*

BIOCELL, Vol.48, No.2, pp. 173-189, 2024, DOI:10.32604/biocell.2023.043664

Abstract Mesenchymal stem cells (MSCs) have been proposed in regenerative medicine, especially for angiogenic purposes, due to their potential to self-renew, differentiate, and regulate the microenvironment. Peripheral vascular diseases, which are associated with reduced blood supply, have been treated but not cured. An effective therapy is to recover blood supply via vessel regeneration in the affected area, and MSCs appear to be promising for such conditions. Several studies aimed to explore the role of MSCs in performing angiogenesis and have revealed numerous potential methods to enhance MSC capacity in vessel formation. Efforts have been made to modify standard MSCs to optimize… More > Graphic Abstract

JINGLONG CAO^1,#, SHUMEI LI^2,#, TONG ZHANG^1,#, JIAN LIU¹, WENSHUANG HOU¹, ANQI WANG¹, CHANG WANG^3,4,*, CHENGHAO JIN^1,3,5,*

BIOCELL, Vol.48, No.2, pp. 313-325, 2024, DOI:10.32604/biocell.2023.043474

Abstract Background: Valtrate (Val) was extracted from the Valeriana jatamansi Jones plant, had good antitumor activity. However, its precise molecular mechanism in cancer cells was still unclear. This study investigated the effect of Val on gastric cancer (GC) cells and its potential molecular mechanism. Methods: Cell viability was examined by CCK-8 assay. Cell cycle, apoptosis, and Reactive oxygen species (ROS) level were analyzed by flow cytometry. The migration effect of Val on AGS cells was analyzed by transwell and wound-healing assay. The expression levels of proteins were analyzed by western blot. Results: The cell viability assay results demonstrated that Val significantly… More > Graphic Abstract

DAIXIN ZHAO¹, QINGYU WANG², JIANBO WANG^1,*

BIOCELL, Vol.48, No.2, pp. 271-281, 2024, DOI:10.32604/biocell.2023.029094

Abstract Background: Esophageal cancer (ESCA) is a common digestive tract tumor. As a result, optimization of the early diagnosis of ESCA and identifying the contributing prognostic genes is urgently required. Herein, the prognosis of mitochondrial autophagy-related genes was analyzed in different subtypes of ESCA, and prognostic models were constructed to identify the immune cell infiltration with significant differences between subtypes. Methods: The Cancer Genome Atlas database was searched to download 185 ESCA samples, covering gene expression level data and clinical follow-up data, and 179 samples from the Gene Expression Omnibus database for subsequent validation analysis. The consensus Cluster Plus analysis method… More >