Mohammed Lafi^1,*, Bilal Hawashin², Shadi AlZu’ bi³

CMES-Computer Modeling in Engineering & Sciences, Vol., , DOI:10.32604/cmes.2021.013026

Abstract Most software systems have different stakeholders with a variety of concerns. The process of collecting requirements from a large number of stakeholders is vital but challenging. We propose an efficient, automatic approach to collecting requirements from different stakeholders’ responses to a specific question. We use natural language processing techniques to get the stakeholder response that represents most other stakeholders’ responses. This study improves existing practices in three ways: Firstly, it reduces the human effort needed to collect the requirements; secondly, it reduces the time required to carry out this task with a large number of stakeholders; thirdly, it underlines the… More >

TIECHENG SUN^1,#, YANDONG ZHANG^1,#, JIANHUA LI^3,#, HONG YU¹, LINGLI SONG¹, XINPING SUN¹, XI CHEN^2,*, LI TIAN^1,2,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014694

Abstract This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in total motility and progressive motility compared to… More >

BENJIANG QIAN¹, YOUFENG HUANG², ZHENQIANG QIU², XIAOYAN YING³, GUANG YANG³, HUIZHANG LI^2,*, JIANMING TAN^1,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014633

Abstract Tet methylcytosine dioxygenase 2 (TET2) acts as an antioncogene that is investigated in different cancers. But the effects of TET2 in renal cell cancer (RCC) is still known little. Here, quantitative real-time PCR (qRT-PCR), Western blot, and immunofluorescence were performed to exam gene and protein expression. Cell proliferation was measured using Cell Counting Kit-8 (CCK-8). Transwell assay was performed to detect cell metastasis viability. Flow cytometry was performed to analyze the cell cycle and cell apoptosis. The effects of TET2 on RCC growth in vivo was analyzed using a mouse xenograft model.We found that TET2 and miR-200c were decreased in… More >

YASHVI SHARMA, SUCHI GUPTA, SUJATA MOHANTY^*,

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014621

Abstract In these times of despair when a nano-sized organism, the SARS-CoV-2, has rendered the human race helpless, made the global health status decline, and drowned the world economy, a ray of hope comes from another nano-sized particle, the exosome. The potential of mesenchymal stem cells has already been established in COVID-19; however, cell-based therapy has its risks. We thereby propose cell-free therapy using stem cells-derived exosomes to fight against COVID-19, as they can be a game-changer owing to their immunomodulatory nature, which combats the cytokine storm characterizing this disease, and their practical efficiency, which will realistically aid large access to… More >

JIGANG LEI^1,#, ZHENYAO XU^2,#, SUKE LI¹, MENG LI¹, ZHIKAI WANG³, PING LI¹, JING WANG¹, YINGLU CHEN¹, XIAOLE SONG¹, CHENGJIE REN¹, MEIPING SHEN¹, CHENGXIANG DAI^1,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014569

Abstract Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3% around the world, involving increased keratinocyte proliferation. Indeed, Th17 cells and IL-17 play critical roles in the pathogenesis of psoriasis. The monoclonal antibodies against cytokines have been shown to have effectively immunosuppressive effects on human psoriasis. However, there are still some patients that have no response to these treatments. Some patients have even serious side-effects which may affect their life. Mesenchymal stem cells have the ability of immunosuppressive and anti-inflammatory effects, which may be an alternative therapy with more safety and efficacy for human psoriasis. Moreover, the… More >

JINGRONG SONG^#, JUAN KANG^#, WEI LV, YAN DONG, XIAOYING ZHANG^*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014453

Abstract Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness (AHR), inflammatory cell infiltration, and airway remodeling. F-box/WD repeat-containing protein 7 (FBXW7), an E3 ubiquitin ligase, is required for various endothelial functions, such as cell migration, inflammation, and endothelial integrity. This study aimed to investigate the role of FBXW7 in lipopolysaccharide (LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro. By using lentivirus-based technology, FBXW7 was overexpressed or silenced (24 h) in human bronchial epithelial (16HBE) cells, which were treated with LPS or not (24 h). Immunoprecipitation (IP) detection and Western blot analysis were used to evaluate… More >

KAVITHA GOVARTHANAN^1,*, PIYUSH KUMAR GUPTA², BINITA ZIPPORAHE¹, REKHA GAHTORI³, SOUMYA PANDIT², RAM PRASAD^4,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014441

Abstract Stem cells constitute the source of cells that replenishes the worn out or damaged cells in our tissue and enable the tissue to carry out the destined function. Tissue-specific stem cells are compartmentalized in a niche, which keeps the stem cells under quiescent condition. Thus, understanding the molecular events driving the successful differentiation of stem cells into several lineages is essential for its better manipulation of human applications. Given the developmental aspects of the cell, the cellular function is greatly dependent on the epigenomics signature that in turn governs the expression profile of the cell. The stable inheritance of the… More >

ZIXUAN WANG^1,#, HONGMEI PENG^2,#, WEI SHI^4,#, LU GAN¹, LIPING ZHONG¹, JIAN HE¹, LINLIN XIE¹, PAN WU¹, YONGXIANG ZHAO¹, ZHIMING DENG^3,*, HONGLIANG TANG⁴, YONG HUANG^1,3,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014439

Abstract Although great achievements have been made in the past decades in medicine, cancer remains a worldwide public health issue. Surgery is usually accompanied by shortcomings such as residual lesions and poor treatment effects, and the successive appearance of other treatment methods, such as radiotherapy and chemotherapy, has not changed the postoperative recurrence rate, toxicity, and side effects. However, the advent of photodynamic therapy has greatly improved this situation. Photodynamic therapy is an emerging tumor diagnosis and treatment technology with good application prospects, photodynamic therapy uses a specific wavelength of light to excite a photosensitizer to generate reactive oxygen species, damage… More >

MAYSON H. ALKHATIB^1,2,*, SALWA M. AL-HASHEMI¹, HANA M. GASHLAN¹

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014349

Abstract Incorporation of etoposide (ETP) into nanoemulsion (NE) containing polyunsaturated fatty acids (PUFAs) may potentially augment its antiproliferation effect on the cancer cells. The current study aimed to examine the in vitro antitumor activity of a novel formulation (ETP-BC/EP-NE) produced by combining the anticancer drug (ETP) with NE (BC/EP-NE) consisting of the black currant seed and organic evening primrose oils. The produced formulas were physically characterized using zetasizer measurements. Their cytotoxic effect was testified at concentrations ranges from 0.0001 to 5 μM using CCK-8. Apoptotic and anti-invasion effects were evaluated using the assays of mitochondrial membrane potential, annexin V-FITC double staining,… More >

HONG ZHOU^1,*, XIAOFENG JIA^1,2, FAN YANG¹

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014193

Abstract Uncontrolled proliferation is a hallmark of cancer cells, yet the molecular mechanisms that contribute to this proliferation are unclear. Therapeutic treatment of cancer is suboptimal in many cases, with no accurate index by which to evaluate the success of treatment or patient prognosis. In this study, we explored the protein levels of nuclear phosphoeIF4E in acute myeloid leukemia (AML) cell lines and primary leukemia samples by Western blot and immunofluorescence and as well analyzed transcriptomes by RNA-seq. We found nuclear phospho-eIF4E, an exporter of oncogenic mRNAs, to be abundant in AML. Further, nuclear phospho-eIF4E abundance was significantly associated with tumor… More >