S. J. Reyes-Zambrano^1,†, M. L. Ramírez-Merchant^1,†, C. Arias-Castro², M. A. Rodríguez-Mendiola², C. A. Lecona-Guzmán¹, V. M. Ruíz-Valdiviezo¹, D. González-Mendoza³, F. A. Gutiérrez-Miceli^1,*

Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 277-284, 2019, DOI:10.32604/phyton.2019.06504

Abstract A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with… More >

V. Mendez-Trujillo¹, B. Valdez-Salas², M. Carrillo-Beltran², M. A. Curiel-Alvarez², O. Tzintzun-Camacho³, C. Ceceña-Duran³ and D. Gonzalez-Mendoza^3,*

Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 269-275, 2019, DOI:10.32604/phyton.2019.07316

Abstract Phenacoccus solenopsis has been recognized as an aggressively invasive species on cotton plants in different countries. This study was conducted to investigate the effect of green synthesized Cu/Zn-nanoparticles using aqueous leaf extract of Prosopis juliflora (mezquite) against P. solenopsis. The scanning electron microscope (SEM) showed bimetallic nanoparticles of Cu/Zn-NPs with spherical shape with varying size of 74.33 nm to 59.46 nm. More than 30% mortality of P. solenopsis was observed with Cu/Zn-nanoparticles (100 ppm) at 96 hours after treatment. Negligible mortality of P. solenopsis was recorded with Cu/Zn solution (100 ppm) and aqueous P. juliflora extracts. The results of the… More >

Lei Chu, H. M. K. Darshika Hennayake, Haijun Sun*

Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 261-267, 2019, DOI:10.32604/phyton.2019.07791

Abstract Intensive practices in forest soils result in dramatic nitrogen (N) losses, particularly ammonia (NH₃) volatilization, to adjacent environmental areas. A soil column experiment was conducted to evaluate the effect of bamboo biochar on NH₃ volatilization from tea garden and bamboo forest soils. The results showed that biochar amendment effectively reduced NH₃ volatilization from tea garden and bamboo forest soil by 79.2% and 75.5%, respectively. The soil pH values increased by 0.53-0.61 units after biochar application. The NH₄⁺-N and total N of both soils were 13.8-29.7% and 34.0-41.9% higher under the biochar treatments than under the control treatment, respectively. In addition,… More >

Abdulkareem Younis SULEIMAN¹, Intisar Salim PITY², Mohammed R MOHAMMED², Bashar Abduljabar HASSAWI²

BIOCELL, Vol.43, No.3, pp. 175-181, 2019, DOI:10.32604/biocell.2019.06884

Abstract Ovarian lesions are commonly encountered pathologies that cannot be categorized clinicoradiologically. Definite diagnosis is of great importance for therapeutic and prognostic purposes. Histopathology gives accurate diagnosis in most cases. Few cases need supportive tests like immunohistochemistry. Objective: to study the histomorphological diversity of ovarian lesions, their age and location in North of Iraq (Mosul and Duhok). Patients and methods: In the period extended from January 2008 to December 2011, 161 cases of ovarian lesions were collected from pathology departments in Azadi General Hospital “Duhok” and Al-Jamhori Teaching Hospital “Mosul”. Automated tissue processor was used for histologic study and Streptavidin-biotin method… More >

Atif Kamil¹, Mubarak Ali Khan¹, Muhammad AAsim², Nadir Zaman Khan², Raham Sher Khan¹, Muhsin Jamal³, Waqar Ahmad⁴, Mir Azam Khan⁴, Fazal Jalil⁴

BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729

Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in conditions like glucose starvation and viral infection. Recently ERp57 was shown… More >

I-L. Chang¹, W.-C. Ding

CMES-Computer Modeling in Engineering & Sciences, Vol.68, No.3, pp. 297-312, 2010, DOI:10.3970/cmes.2010.068.297

Abstract Molecular dynamics method incorporating with parallel computing technique was employed to study the mechanical properties of textured polycrystalline nanofilms with fixed out-of-plane normal direction. The grain size and film thickness effects on both the elastic and plastic properties of the copper nanofilm were examined. It is shown from the simulation that the elastic and plastic properties (i.e., Young’s modulus, Poisson’s ratio, biaxial yield stress, etc) of textured polycrystalline nanofilms depend on the grain size and the film thickness. As the grain size of the nanofilm become larger, the Young’s modulus and biaxial yield stress increase but the Poisson’s ratio decreases.… More >

Xiaoqin Zhang, Tao Tong, Bin Tian, Yunxia Fang, Jiangjie Pan, Junjun Zheng and Dawei Xue*

Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 253-260, 2019, DOI:10.32604/phyton.2019.06143

Abstract Barley (Hordeum vulgare L.) is one of the most Aluminum (Al) sensitive cereal species. In this study, the physiological, biochemical, and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms. The results showed that superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were inhibited to different degrees following Al exposure. The MDA content also significantly increased with increasing Al concentrations. SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile, and the genomic template stability (GTS) decreased with increasing Al concentration and duration. These… More >

Dhouha Belhaj Sghaier¹, Insaf Bankaji¹, Sylvia Pedro², Isabel Caçador², Noomene Sleimi^1,*

Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 239-252, 2019, DOI:10.32604/phyton.2019.06887

Abstract The lack of knowledge of plant tolerance and differential response to aluminum (Al) encouraged many researchers, in the last decade, to elucidate Al toxicity and tolerance mechanisms. The current study reported the impact of Al, a toxic element with negative effects on plant growth and development, in halophytic plant Tamarix gallica. Plants were subjected to different Al concentrations (0, 200, 500 and 800 μM) with or without NaCl (200 mM) supplementation. Growth, photosynthesis and mineral content were assessed. Al stress had a significant decrease on shoots’ biomass production between 19 to 41%, and a little variation on chlorophyll content and… More >

Jai Hak Park¹, Maan Won Kim², Gennadiy P. Nikishkov³

CMES-Computer Modeling in Engineering & Sciences, Vol.68, No.3, pp. 269-296, 2010, DOI:10.3970/cmes.2010.068.269

Abstract A SGBEM-FEM alternating method had been proposed by Nikishkov, Park and Atluri for the analysis of three-dimensional planar and non-planar cracks and their growth. In the method, the symmetric Galerkin boundary element method is used for the crack solution in an infinite body and the finite element method is used to perform stress analysis for the uncracked body only. In this paper the method is extended further to analyze through-thickness cracks. Adequate shape of boundary element mesh is examined and it is found that the fictitious portion of the boundary element mesh, which is located outside the body, plays an… More >

Mahmoud MOUSTAFA^1,2 , Saad ALAMRI¹, Tarek TAHA³, Ali SHATI¹, Sulaiman ALRUMMAN¹, Mohamed ALKAHTANI¹

BIOCELL, Vol.43, No.3, pp. 155-166, 2019, DOI:10.32604/biocell.2019.06814

Abstract The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding peptide (SBP) found in the germinated embryos of Pisum sativum L. var. Alaska. The pea (P. sativum var. Alaska) embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated embryos, but not in the cotyledons. The total RNA was isolated from embryos of P. sativum but could not be isolated from the cotyledons. The partial nucleotides sequences of 66 kDa SBPs of embryonic stalk (P. sativum var. Alaska) were cloned and identified using pMOSBlue vector. 66 kDa… More >