Xiaomei Zhang, Junliang Li, Bin Liang^*

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014962

Abstract Greenhouse vegetable cultivation (GVC) is an example of intensive agriculture aiming to increase crop yields by extending cultivation seasons and intensifying agricultural input. Compared with cropland, studies on the effects of farming management regimes on soil microorganisms of the GVC system are rare, and our knowledge is limited. In the present study, we assessed the impacts of different long-term fertilization regimes on soil fungal community structure changes in a greenhouse that has been applied in tomato (Solanum lycopersicum L.) cultivation for 11 consecutive years. Results showed that, when taking the non-fertilizer treatment of CK as a benchmark, both treatments of… More >

Maricela Apáez-Barrios¹, José Alberto Salvador Escalante-Estrada², Patricio Apáez-Barrios^1,*, Yurixhi Atenea Raya-Montaño³, Juan Carlos Álvarez-Hernández¹

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014567

Abstract Chickpea contains high levels of protein, vitamins and minerals. Acceptable chickpea yield is the result of meeting nitrogen and phosphorus requirements. The effect of appropriately meeting such requirements reflects on growth and can easily be evaluated using growth analysis. This research determined: (a) The effect of nitrogen and phosphorus fertilization on phenology, net assimilation rate, number of green leaves, leaf area, leaf area index and leaf area duration; (b) Green chickpea yield and number of pods due to fertilization; and (c) The combination of nitrogen and phosphorus fertilization that yields the most net revenue. Nitrogen and phosphorus fertilization was evaluated;… More >

Qian Sun^1,2, Toshihiko Yamada³, Yulai Han^2,*, Tetsuo Takano^1,*

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.013805

Abstract The lignocellulosic crop Miscanthus spp. has been identified as a good candidate for biomass production. The responses of Miscanthus sinensis Anderss. to salinity were studied to satisfy the needs for high yields in marginal areas and to avoid competition with food production. The results indicated that the relative advantages of the tolerant accession over the sensitive one under saline conditions were associated with restricted Na⁺ accumulation in shoots. Seedlings of two accessions (salt-tolerant ‘JM0119’ and salt-sensitive ‘JM0099’) were subjected to 0 (control), 100, 200, and 300 mM NaCl stress to better understand the salt-induced biochemical responses of genes involved in… More >

Xiaohang He¹, Minjie Liu², Zhengwu Fang¹, Dongfang Ma^1,2,3,*, Yilin Zhou^1,3,*, Junliang Yin¹

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.012726

Abstract Plant AT-rich sequence and zinc-binding protein (PLATZ) is a plant transcription factor that has been studied in corn. PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression. It is involved in the regulation of dehydration tolerance in seeds. In this study, we performed bioinformatics analysis to identify and characterize wheat PLATZ(TaPLATZ)genes. We identified 49 wheat PLATZ genes by searching the wheat genome by using known PLATZ gene sequences from rice, Arabidopsis, and maize. Phylogenetic analysis on PLATZ gene sequences from different species was performed. We found that PLATZs could be divided into three groups. The… More >

Mohammed Lafi^1,*, Bilal Hawashin², Shadi AlZu’ bi³

CMES-Computer Modeling in Engineering & Sciences, Vol., , DOI:10.32604/cmes.2021.013026

Abstract Most software systems have different stakeholders with a variety of concerns. The process of collecting requirements from a large number of stakeholders is vital but challenging. We propose an efficient, automatic approach to collecting requirements from different stakeholders’ responses to a specific question. We use natural language processing techniques to get the stakeholder response that represents most other stakeholders’ responses. This study improves existing practices in three ways: Firstly, it reduces the human effort needed to collect the requirements; secondly, it reduces the time required to carry out this task with a large number of stakeholders; thirdly, it underlines the… More >

TIECHENG SUN^1,#, YANDONG ZHANG^1,#, JIANHUA LI^3,#, HONG YU¹, LINGLI SONG¹, XINPING SUN¹, XI CHEN^2,*, LI TIAN^1,2,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014694

Abstract This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in total motility and progressive motility compared to… More >

BENJIANG QIAN¹, YOUFENG HUANG², ZHENQIANG QIU², XIAOYAN YING³, GUANG YANG³, HUIZHANG LI^2,*, JIANMING TAN^1,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014633

Abstract Tet methylcytosine dioxygenase 2 (TET2) acts as an antioncogene that is investigated in different cancers. But the effects of TET2 in renal cell cancer (RCC) is still known little. Here, quantitative real-time PCR (qRT-PCR), Western blot, and immunofluorescence were performed to exam gene and protein expression. Cell proliferation was measured using Cell Counting Kit-8 (CCK-8). Transwell assay was performed to detect cell metastasis viability. Flow cytometry was performed to analyze the cell cycle and cell apoptosis. The effects of TET2 on RCC growth in vivo was analyzed using a mouse xenograft model.We found that TET2 and miR-200c were decreased in… More >

YASHVI SHARMA, SUCHI GUPTA, SUJATA MOHANTY^*,

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014621

Abstract In these times of despair when a nano-sized organism, the SARS-CoV-2, has rendered the human race helpless, made the global health status decline, and drowned the world economy, a ray of hope comes from another nano-sized particle, the exosome. The potential of mesenchymal stem cells has already been established in COVID-19; however, cell-based therapy has its risks. We thereby propose cell-free therapy using stem cells-derived exosomes to fight against COVID-19, as they can be a game-changer owing to their immunomodulatory nature, which combats the cytokine storm characterizing this disease, and their practical efficiency, which will realistically aid large access to… More >

JIGANG LEI^1,#, ZHENYAO XU^2,#, SUKE LI¹, MENG LI¹, ZHIKAI WANG³, PING LI¹, JING WANG¹, YINGLU CHEN¹, XIAOLE SONG¹, CHENGJIE REN¹, MEIPING SHEN¹, CHENGXIANG DAI^1,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014569

Abstract Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3% around the world, involving increased keratinocyte proliferation. Indeed, Th17 cells and IL-17 play critical roles in the pathogenesis of psoriasis. The monoclonal antibodies against cytokines have been shown to have effectively immunosuppressive effects on human psoriasis. However, there are still some patients that have no response to these treatments. Some patients have even serious side-effects which may affect their life. Mesenchymal stem cells have the ability of immunosuppressive and anti-inflammatory effects, which may be an alternative therapy with more safety and efficacy for human psoriasis. Moreover, the… More >

JINGRONG SONG^#, JUAN KANG^#, WEI LV, YAN DONG, XIAOYING ZHANG^*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014453

Abstract Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness (AHR), inflammatory cell infiltration, and airway remodeling. F-box/WD repeat-containing protein 7 (FBXW7), an E3 ubiquitin ligase, is required for various endothelial functions, such as cell migration, inflammation, and endothelial integrity. This study aimed to investigate the role of FBXW7 in lipopolysaccharide (LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro. By using lentivirus-based technology, FBXW7 was overexpressed or silenced (24 h) in human bronchial epithelial (16HBE) cells, which were treated with LPS or not (24 h). Immunoprecipitation (IP) detection and Western blot analysis were used to evaluate… More >