Jiao Deng^1,2,#, Fen Dong^3,#, Chaoxin Wu¹, Jiali Zhao¹, Hongyou Li¹, Juan Huang¹, Taoxiong Shi¹, Ziye Meng¹, Fang Cai¹, Qingfu Chen^1,*, Pingfang Yang^2,*

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014625

Abstract Common buckwheat (Fagopyrum esculentum), a specialty crop in southwest China, is not only used as a supplement to primary grain crops but also to produce beverages, such as tea and wine. To fully exploit the products made from common buckwheat flower, ultra-performance liquid chromatography–electrospray ionization– tandem mass spectrometry (UPLC–ESI–MS/MS) was conducted to analyze the metabolites in red- (‘Guihong 2’) and white-flowered (‘Fengtian 1’) buckwheat cultivars. A total of 784 metabolites were identified of which flavonoids were the largest group with 191 components, followed by organic acids and derivatives (126), and amino acids and derivatives (95). Besides, dozens of phenylpropanoids, nucleotides… More >

Qi Zhang^1,#, Pengtao Li^2,#, Aiying Liu¹, Shaoqi Li¹, Quanwei Lu², Qun Ge¹, Junwen Li¹, Wankui Gong¹, Xiaoying Deng¹, Haihong Shang^1,3, Yuzhen Shi^1,*, Youlu Yuan^1,3,*

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014437

Abstract Cotton is the most widely cultivated commercial crop producing natural fiber around the world. As a critical trait for fiber quality, fiber strength principally determined during the secondary wall thickening period. Based on the developed BC₅F_3:5 CSSLs (chromosome segment substitution lines) from Gossypium hirsutum CCRI36 × G. barbadense Hai 1, the superior MBI9915 was chosen to construct the secondary segregated population BC₇F₂ with its recurrent parent CCRI36, which was subsequently subjected to Bulk segregant RNA-sequencing (BSR-seq) for rapid identification of candidate genes related to fiber strength. A total of 4 fiber-transcriptome libraries were separately constructed and sequenced, including two parents… More >

Chenggang Liang^1,#, Chunyu Wei^1,#, Yan Wang^1,*, Wujuan Yu¹, Kai Liao¹, Taoxiong Shi¹, Dabing Xiang^2,3, Qijiao Chen¹

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014242

Abstract Rice-Tartary buckwheat (RTB) is a special germplasm of Tartary buckwheat. In this study, the appraisal of taste quality between RTB and conventional Tartary buckwheat (CTB) was presented, and the metabolites in kernels at three typical grain filling stage (GFS) were investigated. Unlike CTB, RTB showed thin shell seeds without longitudinal furrows at maturity, which was easily artificially dehulled. Sense organ test indicated that RTB exhibited better taste quality because of the higher values of appearance, viscosity, taste and summary were appraised. In total, 92 metabolites were identified in kernels using GM-MS metabolomics platform. The levels of most metabolites changed greatly… More >

Yinkai Xi^1,2, Biao Zeng³, Hengyu Huang^1,4,*

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014218

Abstract An efficient regeneration system of Rhynchostylis retusa was established to provide technical reference for the application of tissue culture tube seedlings in production. The mixtures of callus and protocorm from aseptic germination were used as explants. The optimal media of each stage was selected for callus proliferation, protocorm occurrence and growth, rejuvenation and rooting via a single, complete combination and orthogonal experiment. The results showed that the optimal medium for callus proliferation, protocorms occurrence and growth was 1/2 Murashige and Skoog (MS) medium adding 50 g·L⁻¹ banana puree, 0.1 mg·L⁻¹ α-naphthaleneacetic acid (NAA), 1.5 mg·L⁻¹ 6-benzylaminopurine (6-BA) and 1.0 mg·L⁻¹… More >

Tantan Zhang^1,2,#, Xiaoping He^3,#, Binglin Chen^1,#, Longxin He^1,2, Xiangru Tang^1,2,*

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014168

Abstract As one of the most important nutrients for plants, potassium (K) has substantial effects on growth and development of crops. Present study was conducted in three different sites in South China in late season in 2019 with the objective to study the effects of different applied amounts of K fertilizer on yield formation and lodging of rice. Four K fertilizer treatments, K₀: 0 kg potassium oxide (K₂O) ha⁻¹ (control); K₁: 64.20 kg K₂O ha⁻¹ ; K₂: 128.55 kg K₂O ha⁻¹ and K₃ 153.90 kg K₂O ha⁻¹ were applied in the field experiment. The results showed that K₂ and K₃ treatments… More >

Lucía Isabel Chávez-Ortiz¹, José Francisco Morales-Domínguez¹, Araceli Rodríguez-Sahagún², Eugenio Pérez-Molphe-Balch^1,*

Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.012862

Abstract Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^–1 BA, obtaining a mean… More >

AMAYNA ZAKARIA, SYED ABDULLAH IBN ASADUZZAMAN, ZOBAYDA NAHAR, HAFSA JARIN SNIGDHA, TASKINA MURSHED, RASHED NOOR^*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014704

Abstract The extent and aggression of colorectal cancer is a worldwide public health threat. Extensive research has been conducted on the pre-requisites leading to this fatal cancer. An array of genes along with their mutations and the signal transduction pathways leading to the cellular transformation into the cancerous cells have been investigated. Based on the knowledge gained so far, present review shortly discussed the role of the major genes especially those are involved in instigating abnormalities in the cellular cycles, cellular proliferation and differentiation. A simple but novel molecular scheme of the colorectal cancer development has also been plotted. More >

MARLENE BENCHIMOL^1,3,*, ABIGAIL MIRANDA-MAGALHÃES², ANTONIO PEREIRA-NEVES², WANDERLEY DE SOUZA³

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014599

Abstract Helium ion scanning microscopy (HIM) is a novel high-resolution scanning microscopy technique that uses helium ions instead of electrons to form images of the highest quality and resolution, providing a sub-nanometer resolution sputter uncoated biological cell. Here, we took advantage of HIM to explore the cell surface of Tritrichomonas foetus, a protist parasite of cattle that provokes hard infection and abortion in cows. We describe thin protrusions, like nanotubes described in other cells, with different sizes (27 nm to 81 nm in thickness) and various lengths (from 73 nm to 2 µm), as well bulbous structures either budding from the… More >

DONG HAN KIM¹, DONG RAK KWON^1,*, GI-YOUNG PARK¹, YONG SUK MOON²

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014350

Abstract This work aimed to investigate the synergetic therapeutic effects of polydeoxyribonucleotides (PDRN) combined with extracorporeal shock waves therapy (ESWT) and the effects of the therapy according to ESWT sequences on a chronic traumatic full-thickness rotator cuff tear (RCT) in rabbit models. For this purpose, thirty-two rabbits were randomly allocated into 4 groups. An excision was made to create a 5-mm sized full-thickness RCT right proximal to the insertion site on the supraspinatus. After 6 weeks, 4 different procedures (normal saline, Group 1; PDRN injection, Group 2; PDRN injection before ESWT, Group 3; PDRN injection after ESWT, Group 4) were performed.… More >

AMYLLY SANUELLY DA PAZ MARTINS¹, SAMARA BOMFIM GOMES CAMPOS², MARÍLIA OLIVEIRA FONSECA GOULART^1,2,3, FABIANA ANDRÉA MOURA^4,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014332

Abstract Inflammatory bowel disease (IBD), which includes Crohn’s disease and ulcerative colitis, has a not yet completely defined aetiology and is characterized by a progressive chronic inflammation that involves nitroxidative stress and dysbiosis. Extraintestinal manifestations can occur and affect several organs, including the liver and bile ducts, joints, skin, eyes, and less frequently, the heart, brain, and kidneys, increasing the risk of morbidity and mortality. These repercussions may be associated with the activity or severity of IBD. The present review proposes to report and analyse the participation of dysbiosis and nitroxidative stress in the genesis of extraintestinal manifestations, aiming to contribute… More >