Vol.89, No.3, 2020, pp.715-726, doi:10.32604/phyton.2020.09769
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ARTICLE
In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis
  • Gloria Viviana Cerrillo-Rojas1, Mariana Tiscareño-Andrade1, Ana Erika Ochoa-Alfaro2, Eugenio Pérez-Molphe Balch1, Ruth Elena Soria-Guerra2, José Francisco Morales-Domínguez1,*
1 Departamento de Química, Universidad Autónoma de Aguascalientes, Centro de Ciencias Básicas, Ciudad Universitaria, Aguascalientes, 20131, México
2 Facultad de Química, Universidad Autónoma de San Luis Potosí, Zona Universitaria, San Luis Potosí, 78210, México
* Corresponding Author: José Francisco Morales-Domínguez. Email: jfmoral@correo.uaa.mx
Received 20 January 2020; Accepted 20 February 2020; Issue published 22 June 2020
Abstract
Halophytes are an excellent choice for the study of genes conferring salt tolerance to salt-sensitive plants and, they are suitable for reclamation and remediation of saline soil. We develop an in vitro plant propagation protocol and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis. Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators. The highest number of multiple shoots was on MS medium containing 1 mg/L Benzyladenine (BA) and / or 2 g/L activated carbon with 5.5 ± 06 shoots per explant. The identification and expression analysis of genes involved in glycine betaine (GB) biosynthesis were S-adenosylmethionine synthetase (SAMS), choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), and for proline (Pro) was pyrroline 5-carboxylate synthetase (P5CS). These sequences shared 90–95% of identity with others plant homologous in public databases. The amino acids sequence analysis showed that all these peptides contain some of the conserved motifs of those kinds of enzymes. The qRT-PCR analysis revealed a higher expression of SeBADH, SeCMO, and, SeP5CS genes in the roots and leaves from plants collected in the field in contrast with from in vitro plants. However, the expression level of SeSAMS was higher only in the leaves of plants collected in the field when compared to those cultivated in vitro.
Keywords
Axillary buds; halophyte; proline; glycine betaine; salt-resistant
Cite This Article
Cerrillo-Rojas, G. V., Tiscareño-Andrade, M., Ochoa-Alfaro, A. E., Balch, E. P., Soria-Guerra, R. E. et al. (2020). In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis. Phyton-International Journal of Experimental Botany, 89(3), 715–726.