Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization
- QI SONG1, JUN ZHANG1, QIANG ZHANG1, JING LIU1, KE LV1, JIALU YAO1,2,3,*, YAFENG ZHOU2,3,*
1 Department of Cardiology, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, 215000, China
2 Department of Cardiology, Dushuhu Public Hospital Affiliated to Soochow University, Suzhou, 215000, China
3 Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China
* Address correspondence to: Jialu Yao,
; Yafeng Zhou, 
2 Department of Cardiology, Dushuhu Public Hospital Affiliated to Soochow University, Suzhou, 215000, China
3 Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China
* Address correspondence to: Jialu Yao,
Received 08 July 2020; Accepted 25 September 2020; Issue published 24 December 2020
Abstract
Macrophages play an essential role in the myocardial ischemia-reperfusion injury (MIRI), and the macrophage
shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI. It has been reported that
miR-182 plays an important role in MSC-Exo-associated macrophage polarization. As circBCRC-3 is a newly
discovered circle RNA that worked as a sponge of miR-182, this research aimed to find if circBCRC-3 plays a role in
MSC-Exo-associated macrophage polarization. Firstly, circBCRC-3 was identified by divergent primers in
mesenchymal stem cells (MSCs). Secondly, the exosome of MSCs was isolated and identified by transmission electron
microscopy (TEM), nanoparticle-tracking analysis, and western blotting analysis. The expression level of circBCRC-3
in MSCexos was detected by RT-PCR. Finally, the polarization of the RAW264.7 cell phenotype was analyzed by flow
cytometry. Moreover, we first identified circBCRC-3 in MSCs. The results further confirmed that MSCexo could
effectively shift the macrophage polarization state from M1 towards the M2 phenotype, which indicated its role
in MIRI cure.
Keywords
Exosome, CircBCRC-3, Mesenchymal stem cell, Myocardial ischemia-reperfusion injury
Cite This Article
SONG, Q., ZHANG, J., ZHANG, Q., LIU, J., LV, K. et al. (2020). Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization. BIOCELL, 44(4), 623–629.
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