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ARTICLE
Nicotine and menthol independently exert neuroprotective effects against cisplatin- or amyloid- toxicity by upregulating Bcl-xl via JNK activation in SH-SY5Y cells
YIBIN RUAN1, ZHONGMING XIE2, QIONG LIU2, LIXIAO ZHANG2, XIKUI HAN2, XIAOYAN LIAO2, JIAN LIU1,*, FENGGUANG GAO2,*
1 Technology Center, China Tobacco Guizhou Industrial Co., Ltd., Guiyang, 550003, China
2 Department of Basic Medical Sciences, School of Medicine, Xiamen University, Xiamen, 361102, China
* Address correspondence to: Jian Liu, ; Fengguang Gao,
BIOCELL 2021, 45(4), 1059-1067. https://doi.org/10.32604/biocell.2021.015261
Received 04 December 2020; Accepted 08 February 2021; Issue published 22 April 2021
Abstract
Nicotine and menthol, agonists of nicotinic acetylcholine receptor (nAChR) and transient receptor potential
melastatin type 8 (TRPM8), serve important roles in the prevention of cell death-involved neurodegenerative diseases.
However, the potential synergistic effects of nicotine and menthol on anti-apoptotic ability are still uncertain. In the
present study, the potential synergistic effects of nicotine and menthol on cisplatin or amyloid β
1-42 induced cell
model of the neurodegenerative diseases were explored by assessing cell viability, TNF-α expression, caspase-3
activation, and the collapse of mitochondrial membrane potential in human SH-SY5Y neuroblastoma cells. Statistical
significance was tested using Student’s
t-test or one-way ANOVA with
post hoc Newman-Keuls test. The results
showed that: Firstly, SH-SY5Y cell viability was obviously increased by the treatments with nicotine and menthol.
Secondly, nicotine and menthol independently alleviated cisplatin or amyloid β
1-42 induced TNF-α up-regulation.
Thirdly, nicotine and menthol abrogated the effect of cisplatin and amyloid β
25-35 on caspase-3 activation.
Interestingly, the effect of cisplatin and amyloid β
1-42 on the collapse of mitochondrial membrane potential was
efficiently attenuated by nicotine and menthol treatments. Most importantly, the inhibition of c-jun kinase (JNK)
activation abolished the effect of cisplatin, and amyloid β
1-42 stimulated Bcl-xl expression. All these findings indicate
that nicotine and menthol independently exert neuroprotective effects by upregulating Bcl-xl
via JNK activation.
Nicotine and menthol augmented Bcl-xl expression and JNK phosphorylation, and thus they are potential therapeutic
targets for altering the progress of neurodegenerative diseases.
Keywords
Cite This Article
APA Style
RUAN, Y., XIE, Z., LIU, Q., ZHANG, L., HAN, X. et al. (2021). Nicotine and menthol independently exert neuroprotective effects against cisplatin- or amyloid- toxicity by upregulating bcl-xl via JNK activation in SH-SY5Y cells. BIOCELL, 45(4), 1059-1067. https://doi.org/10.32604/biocell.2021.015261
Vancouver Style
RUAN Y, XIE Z, LIU Q, ZHANG L, HAN X, LIAO X, et al. Nicotine and menthol independently exert neuroprotective effects against cisplatin- or amyloid- toxicity by upregulating bcl-xl via JNK activation in SH-SY5Y cells. BIOCELL . 2021;45(4):1059-1067 https://doi.org/10.32604/biocell.2021.015261
IEEE Style
Y. RUAN et al., "Nicotine and menthol independently exert neuroprotective effects against cisplatin- or amyloid- toxicity by upregulating Bcl-xl via JNK activation in SH-SY5Y cells," BIOCELL , vol. 45, no. 4, pp. 1059-1067. 2021. https://doi.org/10.32604/biocell.2021.015261
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