Open Access

ARTICLE

Wnt3a-induced ST2 decellularized matrix ornamented PCL scaffold for bone tissue engineering

XIAOFANG WANG¹, XIAOLIN TU^1,2,*, YUFEI MA¹, JIE CHEN¹, YANG SONG³, GUANGLIANG LIU¹

1 Laboratory of Skeletal Development and Regeneration, The Institute of Life Science, Chongqing Medical University, Chongqing, 400016, China
2 Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN46202, USA
3 Bioengineering Collage, Chongqing University, Chongqing, 400044, China

* Corresponding Author: XIAOLIN TU. Email:

BIOCELL 2022, 46(9), 2089-2099. https://doi.org/10.32604/biocell.2022.020069

Received 18 November 2021; Accepted 03 January 2022; Issue published 18 May 2022

Abstract

The limited bioactivity of scaffold materials is an important factor that restricts the development of bone tissue engineering. Wnt3a activates the classic Wnt/β-catenin signaling pathway which effects bone growth and development by the accumulation of β-catenin in the nucleus. In this study, we fabricated 3D printed PCL scaffold with Wnt3a-induced murine bone marrow-derived stromal cell line ST2 decellularized matrix (Wnt3a-ST2-dCM-PCL) and ST2 decellularized matrix (ST2-dCM-PCL) by freeze-thaw cycle and DNase decellularization treatment which efficiently decellularized >90% DNA while preserved most protein. Compared to ST2-dCM-PCL, Wnt3a-ST2-dCM-PCL significantly enhanced newly-seeded ST2 proliferation, osteogenic differentiation and upregulated osteogenic marker genes alkaline phosphatase (Alp), Runx2, type I collagen (Col 1) and osteocalcin (Ocn) mRNA expression. After 14 days of osteogenic induction, Wnt3a-ST2-dCM-PCL promoted ST2 mineralization. These results demonstrated that Wnt3a-induced ST2 decellularized matrix improve scaffold materials’ osteoinductivity and osteoconductivity.

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Keywords

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