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Inhibition of H2O2-induced apoptosis of GC2-spg cells by functionalized selenium nanoparticles with lentinan through ROS-mediated ERK/p53 signaling pathways

MIAOMIAO LI1,#, DANYANG CHEN2,#, JUNYI KE1, RUILIN ZHENG2, JINGYAO SU2, ZILIN ZHENG1, JIEYI LUO1, HANRAN MAI1, FAN JIANG1, YANXIA QU1, XIAOQIONG GU1, BING ZHU2, YINGHUA LI2,*, LIANDONG ZUO1,*
1 Department of Andrology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510120, China
2 Center Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510120, China
* Corresponding Authors: YINGHUA LI. Email: ; LIANDONG ZUO. Email:

BIOCELL 2023, 47(2), 401-408. https://doi.org/10.32604/biocell.2023.025154

Received 24 June 2022; Accepted 15 August 2022; Issue published 18 November 2022

Abstract

A H2O2-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nano-selenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of selenium-containing antioxidant enzymes induced by oxidative stress. A safe and effective nano-drug system of functionalized selenium-containing nanoparticles (SeNPs) was developed with lentinan (LNT) (SeNPs@LNT). Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT (1, 2, 4, 8, 16, 32 μM) for 24–72 h to evaluate the cytotoxicity of selenium. GC2-spg cells were randomly divided into the following groups: control, hydrogen peroxide (H2O2), SeNPs@LNT, and H2O2+SeNPs@LNT groups. H2O2+SeNPs@LNT group was pretreated with SeNPs@LNT 4 μM for 12 h, followed by H2O2 600 μM for 8 h. The cell viability decreased in the H2O2 group and increased significantly in the SeNPs@LNT group. Compared with the H2O2 group, the SeNPs@LNT+H2O2 group exhibited obvious red fluorescence, indicating a higher level of mitochondrial membrane potential. The content of intracellular reactive oxygen species (ROS) in the SeNPs@LNT group reduced significantly, and the intensity of green fluorescence in the SeNPs@LNT+H2O2 group decreased significantly compared with the H2O2 group, indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress. The activity of GPx and SOD increased significantly in the SeNPs@LNT group. The expression of p53 decreased significantly under the intervention of nano-selenium, and GPx1 expression increased. In the oxidative stress group, the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups. Thus, SeNPs@LNT can promote GC2-spg cell proliferation, improve GPx and SOD activities, remove intracellular ROS, and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels. SeNPs@LNT has good biological activity and antioxidant effect, which can be used to protect the male reproductive system from oxidative stress.

Keywords

Selenium nanoparticles; Lentinan; ROS; Antioxidant

Cite This Article

LI, M., CHEN, D., KE, J., ZHENG, R., SU, J. et al. (2023). Inhibition of H2O2-induced apoptosis of GC2-spg cells by functionalized selenium nanoparticles with lentinan through ROS-mediated ERK/p53 signaling pathways. BIOCELL, 47(2), 401–408.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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